The National University of the Patagonia San Juan Bosco (Spanish: Universidad Nacional de la Patagonia San Juan Bosco) is a higher education establishment in Patagonia, southern Argentina. It was created on February 25, 1980, by law 22.713, as the merge of two national universities: the "Universidad de San Juan Bosco" and "Universidad Nacional de la Patagonia". It is named after San Juan Bosco, patron saint of the area.The university has four schools - Engineering, Economy, Humanities, Legal, and Natural Sciences, spread over several cities in Patagonia: Puerto Madryn, Trelew, Esquel, Comodoro Rivadavia, and Ushuaia. The central faculty is located in Comodoro Rivadavia.The University has as of 2005[update] 14,000 students, with 5000 in the main school.In 2015, the university opened to the inhabitants of the Falkland Islands. The academic program was translated into English, language courses were designed and a system of special scholarship was established. Thus, National University of the Patagonia San Juan Bosco became the first Argentine university to extend their academic offer to Falkland Islanders.
Con el propósito de relevar la disponibilidad de fuentes de néctar y polen en el noroeste de Santa Cruz se siguió la fenología de floración de 113 especies del valle de Los Antiguos. Las plantas relevadas pertenecen a 36 familias, de ellas, las más representadas en la oferta de floración fueron Asteraceae, Fabaceae, Brassicaceae y Rosaceae. Del total de especies relevadas, 47 son nativas, 40 naturalizadas y 26 cultivadas. El pico máximo en la oferta de floración se registró a fin de noviembre y principios de diciembre con el mayor número de especies en floración plena. Las plantas introducidas dominaron las floraciones durante la mayor parte del período apícola, con excepción de la segunda quincena de octubre y el mes de noviembre en que predominaron las nativas. Se registraron 32 especies muy visitadas por Apis mellifera L., tres de ellas pertenecen a la flora característica de la estepa: Mulinum spinosum (Cav.) Pers., Phacelia secunda J. F. Gmel. y Schinus marchandii F. A. Barkley
Oleaginous Rhodococcus strains can degrade diverse compounds and synthesize and accumulate large amounts of intracellular lipids, making them attractive platforms for biotechnological applications. However, efficient genome editing in Rhodococcus remains challenging, and new molecular tools are needed to advance the understanding of its metabolism, stress responses, and cellular physiology. Here, we describe the nonhomologous end joining (NHEJ) system in rhodococci and implement an efficient genome-editing system based on a CRISPR/Cas9 nuclease approach that utilizes this repair mechanism, eliminating the need for donor DNA templates. In addition, we report the first implementation of a cytidine base-editing (CBE) system, enabling precise single-nucleotide substitutions (C•G → T•A) in oleaginous Rhodococcus strains. Both strategies rely on a dual-plasmid CRISPR platform, resulting in two plasmid sets: pTipCas9/pCA71sgRNA and pTipBE/pCA71sgRNA. These systems enabled high rates of INDEL formation and C•G → T•A base conversions, with efficiencies of 70-80% and 75-85% at native genomic targets, respectively. Finally, for the CBE system, we implemented a Csy4-mediated sgRNA-processing module to support multiplex genome editing, enabling the simultaneous modification of multiple loci. Together, these tools outperform recombination-based approaches and will facilitate the study of complex metabolic pathways and the development of genetic strategies for biotechnological applications in Rhodococcus, while also being transferable to other actinobacteria.
This paper develops an algorithm to determine the critical (minimum) compensation level above which a DFIG-based wind power system can become unstable due to a subsynchronous resonance, depending on the specific combination of the number of in-service wind turbines. The algorithm employs a numerical continuation process that varies the compensation level and the in-service wind turbines. It begins at a point of the stability boundary hypersurface, where the subsynchronous mode has zero real part, and performs concatenated two-parameter numerical continuations of this condition until the global minimum compensation level is reached. The methodology is explained on a simple radial power system and then it is tested using a practical system.
Antimicrobial resistance (AMR) is a major global health concern, and its monitoring in wildlife is essential from a One Health perspective. Here, we obtained bacterial isolates and assessed the AMR profiles and extended-spectrum β-lactamase (ESBL) production of Escherichia coli isolated from fecal samples of wild felids in a protected Atlantic Forest area in southeastern Brazil. We collected 22 fecal samples and identified depositor species through trichological analysis (ocelot, Leopardus pardalis = 18; jaguar, Panthera onca = 1; puma, Puma concolor = 1; Leopardus sp. = 1; unidentified felid = 1). Bacterial isolation was performed using EMB agar and MacConkey agar supplemented with ceftriaxone or meropenem, and isolates were identified by MALDI-TOF MS. From these, 11 E. coli isolates (ocelot = 9; jaguar = 1; puma = 1) were selected to assess AMR using a standardized disk diffusion assay with 15 antimicrobials and to test for ESBL production. In addition to E. coli, the detected bacterial assemblage included Achromobacter micicolens, Acinetobacter baumannii, Acinetobacter nosocomialis, Acinetobacter vivianii, Enterococcus faecalis, Ochrobactrum intermedium and Stenotrophomonas maltophilia. All E. coli isolates were susceptible to all tested antimicrobials, and none showed evidence of ESBL production. These findings may suggest low selective pressure for AMR in the study area, reflecting limited circulation of antimicrobial-resistant bacteria. As free-ranging carnivores may act as sentinels of environmental change and integrate microbiota from prey, this study provides a baseline for monitoring AMR shifts across populations.
Puerto Rawson es un puerto fluvio-marítimo ubicado en el estuario del Río Chubut. Su actividad pesquera ha evolucionado a lo largo de las décadas, transitando distintas fases productivas en función de la explotación de diferentes especies objetivo. Desde la fiebre del cazón en la década de 1940 hasta el actual auge del langostino, el puerto fue escenario de cambios en las embarcaciones, sus tecnologías y su marco regulatorio. Este trabajo analiza la periodización de su historia pesquera basada en registros históricos, informes oficiales y fuentes primarias.
