The selection of appropriate inacti-vating agents is essential to prevent viral in-fections. However, drug susceptibility difers among virus species; therefore, the antiviral spectrum must be understood to select ap-propriate agents. Conventional evaluation systems do not consider the influence of ex-traneous components caused by diferent me-dia and serum concentrations on the inacti-vation reaction. In this study, we elucidated the antiviral spectrum of commonly used hygiene-related and disinfectant ingredients sodium dodecyl sulfate (SDS), didecyldimethylammo-nium chloride (DDAC), ethanol and sodium hypochlorite by comparing the efects of these agents using viral suspensions with or without media components. The culture medium components in the viral suspensions were replaced with distilled water via column chromatography. These agents were tested for their inactivation efects against adenovirus (ADV), feline calicivirus (FCV), enterovirus (ENTV), and minute mouse virus (MVM), including in the presence of culture medium components. Culture medium enhanced the inactivation efects of SDS, DDAC, and ethanol against ADV. This enhancement did not occur against ENTV or MVM. The inactivation efect of sodium hypochlorite against all viruses decreased in the presence of culture medium. Under medium-free conditions, DDAC highly inactivated FCV only but showed a limited activity against ADV and none against ENTV or MVM. SDS inactivated efectively FCV and ADV. Ethanol strongly inactivated FCV and ADV but was inefective against ENTV and MVM. Sodium hypochlorite strongly inactivated all viruses, even at a low concentration of 10 ppm. In summary, the surfactants and ethanol inactivated FCV and ADV, whereas only sodium hypochlorite inactivated ENTV and MVM. The antiviral spec-tra obtained in this study, provide useful guidance for practical infection control against non-envel-oped viruses and for the formulation of preparations containing surfactants, ethanol, or sodium hypochlorite, enabling the selection of appropriate active ingredients and their concentrations ac-cording to virus species and organic load.
