Recherche des hotes intermediaires de B.a. parmi les copepodes d'un lac de Caroline du Nord.
Etude comparative du developpement de metacercaires cultives in vitro dans un milieu artificiel defini complemente soit par du serum de poussin seul, soit par du serum et un extrait muqueux de l'ileon de poussin.
The perimeters of strongylid eggs were digitized electronically, the resulting coordinate point data converted into 8 geometric parameters, and these parameters subjected to stepwise discriminant analysis. This procedure distinguished 92% of Ancylostoma caninum eggs from those of Uncinaria stenocephala and 100% of U. stenocephala eggs from those of A. caninum. When applied to strongylid species infecting sheep, eggs of the following species were correctly classified as follows: Bunostomum trigonocephalum (60%), Chabertia ovina (58%), Cooperia curticei (60%), Haemonchus contortus (67%), Nematodirus battus (98%), N.filicollis (100%), N. spathiger (96%), Oesophagostomum columbianum (72%), Oe. venulosum (100%), Teladorsagia circumcincta (49.5%), Trichostrongylus axei (76%), and T. colubriformis (73%). Comparable accuracy of classification was achieved for equine parasites only after pairs of species having morphometrically identical eggs were pooled before analysis and all of the cyathostomin categories except Gyalocephalus capitatus further pooled after analysis. The final diagnostic categories, "large strongyle," Triodontophorus serratus, T. tenuicollis, "small strongyle," G. capitatus, and Trichostrongylus axei, were correctly classified in 76.6, 74.3, 83.0, 79.1, 100, and 80.0% of the cases, respectively. Haemonchus contortus and T. colubriformis eggs recovered from fresh feces were significantly wider than eggs dissected from the uteri of female worms collected from the alimentary tracts of the same sheep. Thorough mixing of sheep feces and 10% formalin (1 g/30 ml) yielded fixed H. contortus and T. colubriformis eggs that were morphometrically indistinguishable from fresh ones.
