We present a genome assembly from an individual male Trichiura crataegi (Pale Eggar; Arthropoda; Insecta; Lepidoptera; Lasiocampidae). The assembly contains two haplotypes with total lengths of 1 112.64 megabases and 1 115.22 megabases. Most of haplotype 1 (99.87%) is scaffolded into 28 chromosomal pseudomolecules, including the Z sex chromosome. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 17.03 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.
We present a genome assembly of Filago lutescens (Red-tipped Cudweed; Streptophyta; Magnoliopsida; Asterales; Asteraceae). The assembly consists of two haplotypes with total lengths of 648.45 megabases and 646.22 megabases. Most of haplotype 1 (99.77%) is scaffolded into 14 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial sequence has a length of 225.84 kilobases and the plastid genome assembly has a length of 151.54 kilobases. Gene annotation of this assembly on Ensembl identified 38 942 protein-coding genes. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.
We present a genome assembly from an individual male Platyedra subcinerea (Mallow Seed Moth; Arthropoda; Insecta; Lepidoptera; Gelechiidae). The assembly contains two haplotypes with total lengths of 555.70 megabases and 555.31 megabases. Most of haplotype 1 (99.98%) is scaffolded into 30 chromosomal pseudomolecules, including the Z sex chromosome. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.25 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.
We present a genome assembly of Calluna vulgaris (Heather; Streptophyta; Magnoliopsida; Ericales; Ericaceae). The assembly consists of two haplotypes with total lengths of 521.83 megabases and 520.42 megabases. Most of haplotype 1 (97.25%) is scaffolded into 8 chromosomal pseudomolecules. Most of haplotype 2 (98.57%) is scaffolded into 8 chromosomal pseudomolecules. The mitochondrial sequence has a length of 440.94 kilobases and the plastid genome assembly has a length of 208.47 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.
Introduction Climate change mitigation and adaptation strategies can offer multiple benefits for human health outcomes in the near and long term, often referred to as co-benefits when they are ancillary to the primary objective. These are most obvious when implementation is sufficiently ambitious and responsive to context. However, existing evaluation frameworks do not adequately support measurement of the impacts of complex climate-health interventions, resulting in inconsistent evidence on effectiveness, scale up and transferability, in some cases risking ‘greenwashing’ or maladaptation. Existing guidance on the development and evaluation of complex interventions provides an opportunity to integrate environmental and systems perspectives, and to include diverse voices and ways of knowing. This protocol describes a modified Delphi process to develop comprehensive, equitable evaluation guidance for development and evaluation of climate-health interventions. Methods and Analysis The Delphi process will be constructed of three survey rounds, each with quantitative Likert-scale ratings of key items and a qualitative discussion workshop, designed to resolve disagreements and refine item definitions at the end of each round. Consensus will be defined using median scores, an inter-quartile range of ≤1.5 and an agreement score of 70%. Purposive and snowball sampling will be used to recruit 20–40 panellists to a Delphi panel, aiming for a broad mix of expertise with even representation of perspectives across high-income countries (HICs) and low- and middle-income countries (L&MICs). The Delphi survey tool and process will be informed by a systematic review of existing frameworks and a preliminary survey to elucidate practitioners’ opinions on the gaps in the existing guidance and the priority focal areas for consensus building. Ethics and Dissemination Ethics approval has been obtained from the Observational Research Ethics Committee, London School of Hygiene & Tropical Medicine (LSHTM) (Ref: 32889). Findings will be disseminated via a peer-reviewed publication and online supporting materials.
We present a genome assembly from an individual female Macrocera phalerata (fungus gnat; Arthropoda; Insecta; Diptera; Keroplatidae). The genome sequence has a total length of 572.81 megabases. Most of the assembly (97.46%) is scaffolded into 5 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 20.98 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.
Africa’s mental health information landscape is widely recognised as fragmented, limiting planning, budgeting, and service delivery. In South Africa, this fragmentation is reflected in the collection of depression and anxiety data across multiple institutions and studies, using different instruments, constraining comparability, synthesis, and secondary use. This protocol describes the co-development of an open-access, user-oriented mental health data discovery platform and harmonisation of mental health measures for young people in South Africa (PAMHoYA). The platform is designed to improve the discoverability and comparability of existing mental health data for young people aged 15–24 years, using publicly available South African sources. Through a process of co-development, we will work with lived experience experts of common mental health disorders, researchers including academics, the National Department of Health, and civil society to a) map and integrate available metadata sources in South Africa containing information on depressive and anxiety symptoms; b) design the mental health data search platform; c) retrospectively harmonise mental health measures using Natural Language Processing; and d) make harmonised datasets openly accessible via a data repository. The platform aims to facilitate data findability and instrument metadata harmonisation thereby enabling secondary analysis of large mental health datasets, supporting national and subnational estimates, and identifying gaps in the current mental health data. This has the potential to strengthen mental health research, surveillance, and evidence-informed policy in South Africa. The protocol outlines the platform’s design, stakeholder engagement approach, and planned evaluation.
We present a genome assembly from an individual female Nalassus laevioctostriatus (darkling beetle, Arthropoda; Insecta; Coleoptera; Tenebrionidae). The assembly contains two haplotypes with total lengths of 534.57 megabases and 558.90 megabases. Most of haplotype 1 (89.73%) and haplotype 2 (83.43%) are each scaffolded into 10 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled, with a length of 15.53 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.
We present a genome assembly from a colony sample of Acropora cf. manni (staghorn coral; Cnidaria; Anthozoa; Scleractinia; Acroporidae). The assembly contains two haplotypes with total lengths of 459.26 megabases and 489.30 megabases. Most of haplotype 1 (94.39%) is scaffolded into 14 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 18.48 kilobases. Metagenome binning recovered one bacterial bin from the phylum Chlamydiota.
We present a genome assembly of Petasites hybridus (Butterbur; Streptophyta; Magnoliopsida; Asterales; Asteraceae). The assembly consists of two haplotypes with total lengths of 2 802.87 megabases and 2 756.82 megabases. Most of haplotype 1 (95.61%) is scaffolded into 30 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial sequence has a length of 302.34 kilobases and the plastid genome assembly has a length of 150.22 kilobases. Gene annotation of this assembly on Ensembl identified 41 187 protein-coding genes. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.
Setting Tuberculosis (TB) remains a major global public health challenge, particularly in low-income countries, where delayed diagnosis undermines control efforts. Public engagement and community interventions are crucial to the End-TB Strategy; however, outreach experience documentation remains limited. Objective This study describes five years (2021–2025) of World TB Day outreach activities in Ghana. Design Activities included community education, stakeholder engagement, survivor advocacy, and health screening in schools, healthcare facilities and communities. Educational strategies were culturally tailored using interactive sessions, testimonials, and media. Integrated screening combined TB testing with HIV, malaria, diabetes, and hypertension. Results Over 5,000 individuals received education, with over 2,000 materials distributed yearly. Educational campaigns improved community knowledge of TB symptoms, transmission, prevention, and treatment, increasing voluntary screening. Screening enabled the early detection of TB and co-morbid conditions, reduced barriers, and strengthened referrals. The number of TB cases from outreach areas at a major tertiary hospital increased from 21/404(5%) in 2021 to 50/48(10%) in 2024. Community-centered TB education with integrated screening can improve awareness, reduce stigma, and enhance early case detection. Conclusion World TB Day provides advocacy, but its impact requires continuous engagement in community health programs. These findings offer lessons for strengthening TB awareness in high-burden countries.
We present a genome assembly of Frangula alnus (alder buckthorn; Streptophyta; Magnoliopsida; Rosales; Rhamnaceae). The assembly consists of two haplotypes with total lengths of 279.06 megabases and 281.25 megabases. Most of haplotype 1 (99.91%) is scaffolded into 10 chromosomal pseudomolecules. Most of haplotype 2 (99.91%) is scaffolded into 10 chromosomal pseudomolecules. The mitochondrial sequence has a length of 492.03 kilobases and the plastid genome assembly has a length of 161.23 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.
We present a genome assembly from an individual female Ophion crassicornis (ichneumonid wasp; Arthropoda; Insecta; Hymenoptera; Ichneumonidae). The genome sequence has a total length of 726.34 megabases. Most of the assembly (98.51%) is scaffolded into 13 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 38.03 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.
We present a genome assembly from an individual female Hydrotaea militaris (Arthropoda; Insecta; Diptera; Muscidae). The assembly contains two haplotypes with total lengths of 1 703.03 megabases and 1 760.39 megabases. Most of haplotype 1 (96.2%) is scaffolded into 6 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 18.83 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.
Abstract We present a genome assembly from an individual female Tranosemella praerogator (Arthropoda; Insecta; Hymenoptera; Ichneumonidae). The genome sequence has a total length of 196.25 megabases. Most of the assembly (99.97%) is scaffolded into 12 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 34.05 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.
We present a genome assembly of Jacobaea vulgaris (Common Ragwort; Streptophyta; Magnoliopsida; Asterales; Asteraceae). The assembly consists of two haplotypes with total lengths of 2,027.85 megabases and 2,005.08 megabases. Most of haplotype 1 (95.75%) is scaffolded into 20 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial sequences have lengths of 214.29 and 64.31 kilobases and the plastid genome assembly has a length of 150.84 kilobases. Gene annotation of this assembly on Ensembl identified 36,155 protein-coding genes. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.
Background Multiple sclerosis (MS) is a progressive autoimmune neurodegenerative disorder characterized by inflammation, demyelination, and axonal damage. Current disease-modifying therapies demonstrate limited efficacy with substantial adverse effects. Ayurveda and yoga have demonstrated disease-modifying potential through anti-inflammatory, immunomodulatory, and neuroprotective mechanisms. Objective This study aims to evaluate the efficacy of a three-month Integrated Yoga and Ayurveda Management (IYAM) program as an adjunct to standard care in MS patients. Primary outcomes include clinical disability (EDSS), functional capacity (MSFC), quality of life (MSQOL-54), cognition (AVLT), and psychological parameters (HARS/HDRS). Secondary outcomes encompass neuroimaging (brain volume, connectivity), immune biomarkers (Th1/Th2/Th17/Treg cells, cytokines, telomere length), autonomic function (HRV), and gut microbiome composition. Methods In this assessor-blind randomized controlled superiority trial, 60 MS patients (McDonald criteria 2017, disease duration <2 years) will be recruited from tertiary care settings after informed consent. Participants will be randomly allocated (1:1) to IYAM (Ayurvedic Rasayanas: Lashuna 16 days, Bala 36 days, Shatavari 36 days and 2 days alotted for sadhyovirechana, plus yoga therapy) or standard care. A computer-generated randomization sequence was created using the REDCap randomization module. Group assignments were concealed until participant enrollment, ensuring allocation concealment, with blinded assessors. Assessments at baseline, 45 days and three months include clinical scales, neuropsychological testing, 3 T MRI, immunophenotyping, and microbiome analysis. Intent-to-treat analysis with mixed repeated measures ANOVA. Expected Outcomes Significant improvements in disability, cognition, and quality of life are anticipated in IYAM group, mediated through neuroinflammatory modulation, enhanced neuroprotection, and favorable gut microbiome alterations. Trial Registration Clinical Trials Registry-India (CTRI/2024/12/078605); date of registration: 26 December 2024; dates of clinical trial: January 2025 to December 2027. Protocol Version Version 1.0, October 2025.
We present a genome assembly from an individual female Hydrotaea militaris (Arthropoda; Insecta; Diptera; Muscidae). The assembly contains two haplotypes with total lengths of 1 703.03 megabases and 1 760.39 megabases. Most of haplotype 1 (96.2%) is scaffolded into 6 chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 18.83 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.
Tuberculosis remains a leading global cause of morbidity and mortality. While the World Health Organization has established target product profiles to guide diagnostic development, descriptions of potential use cases for biomarkers and tests remain scattered across diverse guidelines and literature. There is currently no clear synthesis or prioritization of these use cases to guide research and investment. This protocol describes a study aiming to identify, map, and prioritize tuberculosis biomarker use cases to focus development efforts where they can maximize clinical and public health impact. Methods The study will employ a multi-step, iterative process combining a scoping review with a consensus-building exercise. First, a scoping review following the Arksey and O’Malley framework will be conducted across academic databases and global health repositories to identify existing use cases. Identified use cases will be mapped to a framework of tuberculosis stages, including infection, disease, treatment, and post-tuberculosis care. Subsequently, a hybrid RAND/UCLA appropriateness method and modified Delphi process will be used to establish consensus among a diverse group of stakeholders, including researchers, clinicians, industry representatives, and people with lived experience of tuberculosis. Stakeholders will participate in anonymous online surveys to rate the validity and importance of each use case, followed by hybrid in-person and virtual meetings to resolve areas of disagreement. Results The primary outputs will be a consolidated, prioritized list of tuberculosis biomarker use cases. These will be disseminated through a public report, peer-reviewed scientific articles, and conference presentations to inform the global research community and funders. Conclusions By integrating evidence synthesis with structured stakeholder consensus, this project seeks to align biomarker discovery and diagnostic development with the most critical clinical and public health needs, ultimately reducing research waste and supporting the global target of ending the tuberculosis epidemic.
We present a genome assembly from an individual female Macrocera phalerata (fungus gnat; Arthropoda; Insecta; Diptera; Keroplatidae). The genome sequence has a total length of 572.81 megabases. Most of the assembly (97.46%) is scaffolded into 5 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 20.98 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.
