Huaxiibacter chinensis is a recently proposed Enterobacteriaceae species with few public genomes. We report the draft genome of urinary isolate 195099, carrying multiple resistance genes, including blaNDM-5, documenting a carbapenem-resistant clinical isolate of this uncommon species.
We report the draft genomes of two Enterococcus faecium blood isolates: one vancomycin-susceptible and one vancomycin-resistant. The assemblies comprised 2,991,512 bp (7 contigs) for the susceptible strain and 3,305,414 bp (7 contigs) for the resistant strain.
Anaplasma phagocytophilum is an obligate intracellular gram-negative bacterium and etiologic agent of human granulocytic anaplasmosis. A. phagocytophilum genomic sequencing has historically been performed via short-read platforms. Our optimized bacterial isolation protocol combined with Nanopore sequencing produced a single, closed 1,481,805 bp circular A. phagocytophilum strain NCH-1 chromosome.
We present the complete genome sequence of Salmonella enterica subsp. enterica serovar Enteritidis SD191 isolated from Gallus gallus liver in China, harboring plasmid pSE191. The genome reveals multiple antibiotic resistance mechanisms and phenotypic imipenem resistance without canonical genes.
We report the genome sequence of Aleuroclava gordoniae-associated polerovirus (AgAP), identified from the whitefly Aleuroclava gordoniae. The 5,650-nt AgAP genome contains 6 open reading frames. Phylogenetic analysis places AgAP within the genus Polerovirus, which comprises plant-infecting viruses. This study provides a genomic resource for further investigation of virus-insect associations.
We report the draft genome sequence of Enterococcus casseliflavus MBBL_MP4, recovered from healthy bovine milk. The 3.45-Mbp genome assembly comprises 27 contigs and indicates low pathogenic potential, with no acquired antimicrobial resistance or known virulence genes. This genome provides a valuable resource for the genomic characterization of bovine-associated E. casseliflavus.
We report draft genome sequences of Lactiplantibacillus plantarum LP48, Enterococcus durans ED01, and Bacillus subtilis BS01 isolated from artisanal goat cheese. Strains were identified by PCR, Sanger sequencing, and Illumina whole-genome sequencing. Assemblies revealed strain-specific features.
Here, we report the genome assembly and annotation of two clinical isolates of Naegleria fowleri from primary amebic meningoencephalitis cases in Costa Rica in 2020 using Illumina sequencing. Each assembly comprised 37 reference-guided scaffolds, with genome sizes of approximately 28 Mbp and a GC content of 36.87%.
The genome of Aspergillus ustus VKPM-F2030 strain was resequenced and assembled to achieve better quality and consistency of the assembly. It is the largest known genome of Aspergillus section Usti corresponding to 42 Mb and 25 contigs. This work can serve for further genomic investigations of Aspergillus fungi.
Here, we report the complete genome sequence of Sphingobacterium sp. PAMC25397, isolated from Ny-Ålesund, Spitsbergen, Norway. The genome is 5.2 Mb in length with a GC content of 37%. This genome harbors biodegradation-related genes involved in benzoate degradation.
We report the whole-genome sequence of multidrug resistant Escherichia coli RSJ-SAU.Ec.2025 isolated from the nasal swab of a cat in Dhaka, Bangladesh. The assembled genome comprises 5,318,660 bp with 50.5% guanine-cystosine (GC) content, together with putative antibiotic-resistant genes.
The draft genome sequence of Limimaricola soesokkakensis TX01, isolated from the light organ of Anomalops katoptron, which lives in the region of the Banda Islands, Indonesia. The assembled genome is 4,041,441 base pairs in length, distributed across 69 contigs, with a GC content of 67.21%, and encodes 3,823 predicted protein-coding genes.
We report the genome sequence of Breoghania corrubedonensis DSM 23382T isolated from oil-spill contaminated beach sand. The 5,331,589-bp genome with 63.62% G + C encodes 4,746 genes. This reference genome will facilitate experimental studies investigating B. corrubedonensis's role in oil-contaminated marine environments, particularly oil degradation or resistivity.
We report the complete genome sequences of three Pseudomonas isolates recovered from topsoil at the California Botanic Garden. Two isolates share ~99.4% average nucleotide identity, enabling investigation of intraspecies microvariation; the third represents a co-occurring distinct species, together capturing species- and strain-level genomic diversity within a natural soil community.
Metagenomic Coverage and Depth Calculator (Meta-CD) is a convenient, biologist-friendly tool for determining coverage and depth to enhance taxonomic detection, functional profiling, and metagenome-assembled genome (MAG) recovery in metagenomics. It supports experimental design and post-sequencing analysis, modeling how genome size, relative abundance, sequencing depth, and DNA quantity influence detection of target species.
Sediment microbial fuel cells (SMFCs) were constructed using different sediments, and microbial communities were analyzed by 16S rRNA gene amplicon sequencing. Methane concentrations were lower in roadside ditch sediment under SMFC conditions than under control conditions. Methanoperedenaceae was selectively enriched under SMFC conditions, suggesting a potential role in methane oxidation.
The Bacillus cereus group is ecologically diverse but genomically close and is known for its variety of capacities, including its antimicrobial potential. Within a project aiming to find new bioactivity, nine Bacillus strains were sequenced: eight Bacillus thuringiensis and one Bacillus mycoides. Here, we report their genome sequences and various genomic features.
In the current study, we present the genome sequence of Staphylococcus aureus isolated from a patient suffering from an ear infection. The bacterial genome was assembled into 26 contigs, comprising a total of 2,774,206 bp with a GC content of 32.5% and encoded approximately 2,782 genes, including multiple virulence-associated genes.
Barnettozyma species are members of the order Phaffomycetales, which include industrially important fungi. A species having ribosomal DNA similarity to Barnettozyma pratensis, but distinct from it, was isolated during collaborative identification work for a UK industrial source. Here, we report the draft genome sequence of Barnettozyma sp. NCYC 4493.
Rice is facing attacks from emerging diseases caused by fungi of the Curvularia genus. Twenty-one species have been isolated from rice. Of these, 8 species cause brown spot disease, and only 13 complete genome sequences are available. We produced 10 Curvularia genome sequences of strains isolated from diseased rice.
