This is the first genome assembly of a yellow striped stingless bee species Lepidotrigona flavibasis at the chromosomal level. Specimens were collected from Yunnan Province, China, and sequenced using PacBio HiFi long-read (Sequel II), RNA-seq (transcriptome), and Hi-C technologies. The assembly was generated via de novo assembly with HiFiasm, scaffolded to chromosomes using YaHS with Hi-C data, and manually curated in Juicebox. The final 251.74 Mb assembly comprises 16 chromosomes (Contig N50 = 10.74 Mb), exhibiting high continuity and accuracy (94.6% BUSCO completeness). Hi-C interaction maps confirmed distinct chromosomal compartmentalization. The genome contained a total of 42.13 Mb of repetitive sequences, accounting for 14.90% of the entire genome. A total of 10,041 genes were annotated across the whole genome using transcriptomic (RNA-seq) evidence, among which 8, 972 genes were successfully functionally annotated from various databases. The first high-quality reference genome of Lepidotrigona constructed in this study provides a fundamental resource for the evolutionary, ecological and conservation genomics research of stingerless bees.
Muscina stabulans (Diptera: Muscidae) exhibits a saprophagous–predatory dual-niche life history and is widely used as a forensic indicator. However, the lack of a high-quality reference genome has limited phylogenomics, functional genomics, and the genomic interpretation of forensic traits in this lineage. Here, we present the first chromosome-level reference genome for M. stabulans, generated by integrating PacBio HiFi long reads, Illumina short reads, and Hi-C data. The assembly spans 747.50 Mb (scaffold N50 = 136.70 Mb), with 98.99% of the assembled sequence (739.97 Mb) anchored into five pseudochromosomes. BUSCO analysis (n = 1,367) indicates 97.8% completeness (96.3% single-copy and 1.5% duplicated BUSCOs). High assembly quality is supported by high read-mapping rates, per-pseudochromosome BUSCO completeness, a high LTR Assembly Index (19.86), concordant chromosome-scale coverage, terminal telomeric repeats, and GC content consistency with other muscid genomes. Repetitive elements account for 61.33% of the genome, and we annotated 12,693 protein-coding genes. This chromosome-level genome provides a robust foundation for Muscina spp. genomics and comparative studies across Muscidae.
Kidney disease represents a significant clinical challenge, with incidence and mortality rising despite therapeutic advances such as dialysis and transplantation. Among its multifactorial causes, drug-induced nephrotoxicity, particularly from cisplatin, a widely used chemotherapeutic agent, represents a frequent and dose-limiting complication that can trigger both acute injury and progressive damage to the kidney. To address critical gaps in understanding the temporal molecular progression of this toxicity, the present study provides the first integrated time-resolved transcriptomic and proteomic atlas of the renal cortex in two complementary mouse models of cisplatin-induced nephrotoxicity: a single high-dose cisplatin (SHDC) model and a repeated low-dose cisplatin (RLDC) model. Renal cortex samples were systematically collected at various time points and subjected to integrated transcriptomic and proteomic analysis, thereby generating a multi-omics temporal dataset. This dataset comprehensively documents the dynamic molecular trajectories of cisplatin-induced renal injury from acute stress to chronic fibrosis, providing a robust multi-omics foundation for elucidating disease progression mechanisms, offering new insights into biomarkers and potential targets for early intervention in cisplatin nephrotoxicity.
Pelophylax nigromaculatus is an East Asian ranid frog that is commercially cultured in China. We generated a chromosome-level reference assembly using PacBio HiFi, short-read, and Hi-C data. The assembly contains 5.06 Gb of non-gap sequence, of which 4.88 Gb (96.48%) was anchored to 13 chromosome-scale scaffolds. The contig N50 and scaffold N50 are 31.64 Mb and 576.86 Mb, respectively. Genome-mode BUSCO recovered 95.26% of 3,354 vertebrate orthologues. Repetitive sequences span 3.93 Gb (77.61%), and the annotation contains 32,557 protein-coding genes, 31,715 of which have functional assignments. Terminal TTAGGG/CCCTAA arrays were detected at 17 of 26 chromosome ends. The assembly, annotation, and raw sequencing data are publicly available as a chromosome-level genomic resource for P. nigromaculatus.
Lejeuneaceae, comprising 90 genera and approximately 1,900 species, represents the most species-rich family in liverworts and an important functional component of epiphytic plant communities across tropical and subtropical forest ecosystems. Here, we report a pseudochromosome-scale genome assembly of Acrolejeunea sandvicensis (Gottsche) Steph. using a combination of Illumina, PacBio CCS and Hi-C technology. The final assembly spans 352.633 Mbp in total length, with a contig N50 of 230,323 bp. Hi-C chromosome mapping yields eight pseudochromosomes, incorporating 1,897 contigs. The completeness of the genome assembly was evaluated using BUSCO analysis, yielding a completeness score of 91.8% against the bryophyte_odb10 dataset. A total of 164.561 Mbp (46.66%) repeat sequences and 332 non-coding RNAs were predicted, and 90.96% of 15,377 predicted protein-coding genes were functionally annotated. The present genome assembly provides a fundamental and valuable genomic resource for further research into the evolutionary innovations underpinning epiphytic adaptation and the diversification dynamics of leafy liverworts.
