Las infecciones de transmisión sexual es una de las causas mas frecuentes de consulta médica. A nivel mundial se ha visto un aumento en el número de casos, por lo que se hace necesario realizar guías y consensos para la prevención y el tratamiento de estas enfermedades. En el siguiente trabajo, se realiza el conceso sobre prevención de infecciones de transmisión que abarca desde la prevención primaria, la realización de la consulta médica, los tipos de pruebas y el manejo ante una potencial exposición. Se ha desarrollado una adaptación de guías internacionales y nacionales, de manera que sean de fácil aplicabilidad en nuestro territorio nacional, y que faciliten la consulta para los médicos en cualquier nivel de atención.
Syphilis is a sexually transmitted disease caused by Treponema pallidum subsp. pallidum, with a high incidence in patients living with HIV. HIV-syphilis coinfection may present with atypical cutaneous manifestations of secondary syphilis, including pustular, papulosquamous, photodistributed, nodular ulcerative (malignant syphilis), fungal-like, plaque-like, overlapping stages, and nodular forms. We report a case of a man with HIV diagnosed with secondary syphilis presenting with infiltrated erythematous-violaceous plaques. The differential diagnoses included leprosy, erythema nodosum, and cutaneous mastocytosis. Serology and skin biopsy confirmed the diagnosis. The patient received three weekly doses of benzathine penicillin, achieving complete resolution of the lesions after the first administration. At three months, serology showed a fourfold decline in rapid plasma reagin titers, confirming the therapeutic response. Subsequently, after antiretroviral therapy, the patient achieved viral suppression and immunological recovery. This case highlights the importance of recognizing the varied cutaneous manifestations of secondary syphilis in patients with HIV to guide timely diagnosis and adequate management.
Antibiotic combination therapy is widely used in clinical practice; however, its application often lacks a clear understanding of the underlying pharmacological principles. This review critically examines the rationale, benefits, and limitations of combination therapy by integrating evidence from in vitro studies, animal models, and the relevant clinical literature. Elicit was used as a support tool to assist in the organization and analysis of the selected studies. While combinations may broaden antimicrobial coverage, enhance bactericidal activity, or prevent resistance, they can also result in additive or antagonistic interactions that limit their clinical effectiveness. This review emphasizes that antibiotic combinations should not be guided by empirical intuition but approached as a rational strategy supported by well-defined therapeutic goals and strong scientific evidence to optimize clinical outcomes and address antimicrobial resistance with greater precision.
Fecal microbiota transplantation (FMT) is an increasingly indicated therapy for intestinal disorders. This meta-meta-analysis aimed to evaluate the clinical outcomes of FMT in ulcerative colitis, Crohn’s disease, and recurrent CDI. We searched the PubMed online database for meta-analyses of intestinal disorders. After accounting for overlap, 11 publications on CDI and 13 on inflammatory intestinal diseases were obtained. Based on a meta-meta-analysis of these patients, this study quantified the impact of FMT on clinical outcomes. We combined the effects under the random-effects model and pooled the estimates as odds ratios (OR). In recurrent CDI, clinical remission was identified in 86% of patients in the FMT group versus 49% (n=449) in the control group [OR 1.82, CI95% 1.69-1.96, P < 0.0001]. Eleven studies included data from patients with ulcerative colitis in clinical remission, identified in 36% (n=903) of patients in the FMT group versus 16% (n=217) in the control group [OR 3.58, CI95% 2.99-4.28, P < 0.0001]. FMT in patients with Crohn’s disease was not different from that in the controls. FMT is a promising therapy for recurrent CDI and ulcerative colitis; however, more clinical trials are needed, as well as standardization of the FMT technique and preparation.
Introduction: Latent tuberculosis infection (LTBI) is characterized by a sustained immune response to Mycobacterium tuberculosis antigens without clinical, radiographic, or microbiological evidence of active disease. The aim of this study was to assess medical and social risk factors for progression of latent tuberculosis infection (LTBI) to active pulmonary tuberculosis in children with household TB contact. Materials and methods: A prospective study (2022-2024) involved 120 children aged 4-12 with LTBI. Two groups were formed: children who developed active TB (LTBI-to-TB group) and those who remained latent. Inclusion required consent. Clinical, immunological (IL-2, IgM), and social factors were analyzed. Risk was evaluated via relative risk (RR) and 95% confidence intervals. Results: Progression to active TB was linked to bacillary TB contact (RR=6.5), maternal TB (RR=5.2), anemia (RR=3.8), >= 5 ARI episodes/year (RR=26.0), ineffective BCG (RR=3.0), single-parent homes (RR=11.0), territorial TB (RR=4.5), helminthiasis (RR=10.0), chickenpox (RR=5.0), genitourinarydiseases (RR=8.0), ENT pathology (RR=5.7), isoniazid monotherapy (RR=2.7), COVID-19 or HIV exposure (RR=8.0), IL-2 >= 311.1 ng/ml, and IgM >= 1.18 IU/ml. Discussions: Immunological and social factors increase LTBI progression risk. High-risk children need tailored monitoring.
Introduction: Measuring trough serum levels of vancomycin is recommended by current guidelines for monitoring treatment, with values between 15-20 mg/L for severe infections. Various variables are associated with failing to reach effective levels, which this study seeks to determine. This study aimed to identify factors associated with subtherapeutic serum levels, their relationship with hemato-oncological diseases, and other pharmacokinetic variables. Materials and methods: This was an observational case-control study of hospitalized adults in whom serum vancomycin levels were measured. A total of 857 medical records were analyzed, of which 377 met the inclusion criteria of this study. A random selection of 92 cases and 202 controls was made at a 1:2 ratio without matching. Results: The median age was 59 years, and 56.8% of the patients were male. Hemato-oncological diagnoses were present in 23.1% of patients, with non-Hodgkin lymphoma and acute leukemia being the most frequent. Four variables were statistically associated with subtherapeutic levels: hemato-oncological disease (OR 2.12; 95% CI 1.53-4.88; p=0.004), BMI >= 25 kg/m(2) (OR 1.15; 95% CI 1.07-1.21; p<0.001), age >75 years (OR 0.96; 95% CI 0.93-0.96; p<0.001), and creatinine >0.7 mg/dL (OR 0.08; 95% CI 0.01-0.11; p<0.001). Discussion: Hemato-oncological disease and increased body mass index were significantly associated with subtherapeutic vancomycin levels. In contrast, older age and higher creatinine levels acted as protective factors, possibly due to reduced renal clearance. These findings support the need for therapeutic drug monitoring and dose adjustment in oncologic patients.
Introduction: Infections caused by multidrug-resistant microorganisms have increased in the neonatal population, leading to the development of control and prevention strategies, such as rectal screening for multidrug-resistant microorganisms. This study aimed to determine the prevalence of colonization by multidrug-resistant bacteria and its association with clinical factors in patients referred to or previously hospitalized in the NICU, as well as the incidence of healthcare-associated infections caused by these microorganisms. Materials and methods: A retrospective analytical cohort study of patients admitted to the neonatal unit of the San Ignacio University Hospital in Bogot & aacute; between 2016 and 2020, whose inclusion criteria were referral from another institution or previous hospitalization, and who underwent rectal swabbing. For the first objective, the prevalence of colonization and the incidence of healthcare-associated infections by multidrug-resistant bacteria were determined according to colonization status. For the second objective, the independent variables were preexisting conditions, antimicrobial use, invasive procedures, central access, and previous hospitalization duration, and the dependent variable was colonization by multidrug-resistant bacteria. A descriptive analysis of the variables was performed for the first objective, and logistic regression was used for the exploratory analysis of the factors associated with colonization. Results: Of the 722 patients included, 68 had positive swabs (9.42%) in a 40-month period. The main isolates in swabs and healthcare-associated infections (HAIs) were extended-spectrum beta-lactamaseproducing Enterobacteriaceae other than E. coli. Discussion: This study identified a significant prevalence of multidrug-resistant bacterial colonization in newborns with previous hospitalizations or referred to the hospital's NICU using the rectal swab protocol. Healthcare-associated infections were more frequent in patients colonized by these bacteria, highlighting the importance of screening in this population group, as it allows for actions to be taken to prevent cross-infections. The results of this study demonstrate the importance of screening patients with a history of antibiotic use.
Introduction: Travel medicine emerged in the late 1970s and has gained global importance overtime. As international travel from developing countries has increased, Latin American countries have recognized the need for travel medicine services. This study aimed to describe the first twenty-month experience of a private travel medical consultation initiated in Bogota D.C. and Colombia. Materials and methods: This study was conducted at the "Servicios y Asesorias en Infectologia" private healthcare center in Bogota, Colombia, between August 2022 and March 2024. Patients were anonymized, and data were collected using a standardized survey. The patients were categorized into three groups: pre-travel, post-travel, and foreign vaccine consultations. Results: Over 20 months, 52 patients attended travel medicine consultations in Bogota. Of these, 24 were pre-travel, 15 were post-travel, and 13 were foreigners who consulted for vaccination. Most pre-travel patients were middle-aged men, with common destinations in South America and Central America. Typhoid fever and influenza vaccines were frequently administered. Post-travel patients were mostly men, and consultations were mainly for control and recommendations. Foreign individuals sought vaccination, with yellow fever and typhoid fever being the most common. Most consultations were virtual. Discussion: During the first 20 months of the travel medicine service in Bogota, Colombia, 52 patients were cared for, mostly for pre-travel consultations. Despite the initial low number of patients, the service provided essential vaccines and preventive medication. Its impact is expected to grow, improving public health both local persons and travelers.
Introduction: Hemotropic microorganisms, such as Trypanosoma spp., affect many animals, including water buffaloes. Some developing countries have reported trypanocide resistance to Diminazene Aceturate. This study aimed to describe the presence of resistance associated with trypanocide treatment in Colombian water buffaloes. Materials and methods: In this cross-sectional study, 202 individuals were sampled from an endemic area in Colombia. The molecular technique quantitative polymerase chain reaction (qPCR) was performed targeting the 18S gene for Trypanosoma spp., and phylogenetic analysis based on the ITS1 region was performed. Results: Sixty-one animals (30.2%) were positive for Trypanosoma spp. and fifty-two sequences were completely analyzed. Of the sequences, 76.9% (40/ 52) were compatible with T. vivax and 23.1% (12/ 52) with T. theileri; the obtained results did not show homologous sequences for T. evansi. Subsequently, positive animals were treated with Diminazene Aceturate at the recommended dose (7 mg/kg), and a final qPCR was performed one month after the end of the treatment. All animals were negative for Trypanosoma spp. after treatment with the drug. Discussion: Although no resistance to the drug was detected in the animals included in the study, it is necessary to implement continuous surveillance of trypanocidal drug resistance to verify the circulation of resistant strains in Colombia and endemic areas in other countries.
Introduction: Antimicrobial resistance, resulting from the excessive and inappropriate use of drugs, affects health, food production, and raises healthcare costs. This study aimed to evaluate the impact of an antimicrobial stewardship program (ASP) in the intensive care unit (ICU) of a secondary-level hospital. Materials and methods: Aquasi-experimental interrupted time-series study was conducted over 12 months. Hospitalized ICU patients who received antimicrobials were included in the study. The intervention consisted of prospective audits with direct feedback to the prescribers. The outcomes assessed were antimicrobial consumption, healthcare-associated infection (HAI) rates, mortality, and average ICU length of stay. Results: Antimicrobial consumption decreased from 39.35 to 18.17 DDD/100 patient-days. The overall HAI rate declined from 11.2 to 0.99 per 1,000 patient-days. No significant differences were observed in the mean ICU length of stay (6.87 vs. 6.66 days; p=0.89) or crude mortality (264.7 vs. 261.9/1,000 patients). Discussion: Persuasive strategies were effective in reducing the use of carbapenems, vancomycin, and caspofungin. The intervention also decreased overall HAI rates without adverse effects on mortality or ICU stay.These findings support the efficacy and safety of ASPs in critical care settings. Future multicenter studies are warranted to strengthen the external validity of these results.
Introduction: The management of people living with HIV has seen significant advances in recent decades with antiretroviral therapy (ART); however, healthcare professionals continue to face challenges in clinical management, particularly in viral load interpretation and therapeutic decision-making. This study aimed to standardize the definitions and guidelines for the interpretation and management of persistent low-level viremia in people with HIV receiving antiretroviral therapy in Colombia. Materials and methods: A systematic review of the literature addressing four clinical questions was performed. Evidence was classified according to the Oxford Centre for Evidence-Based Medicine (2009). A modified Delphi consensus method was employed, which involved national experts. The strong consensus threshold was defined as reaching agreement among 80% of the participating experts. Results: Eighteen relevant documents were reviewed in this study. Twelve recommendations with strong consensus were developed, focusing on the definition of persistent low-level viremia, targeted genotyping from 200 copies/mL, and the transition to regimens with a high genetic barrier. Discussion: Recent studies suggest that persistently detectable low-level viremia may increase the risk of emerging resistance and virologic failure. This document adopts a more conservative, clinically oriented approach, in line with the recommendations of the DHHS and GeSIDA. The importance of assessing adherence, optimizing regimens, and judiciously using genotyping is emphasized. In conclusion, careful assessment of adherence, use of high genetic barrier regimens, and an individualized approach optimize care for people with HIV with persistent low-level viremia, especially in uncertain clinical scenarios.
Sexually transmitted infections are among the most common reasons for medical consultations, and their global incidence is increasing. This study presents a consensus on prevention, including primary prevention strategies, medical consultation, diagnostic testing, and management after exposure. International and national guidelines were adapted to facilitate their implementation in the country and serve as practical references for physicians at all levels of care.
Non-tuberculous mycobacterial infections, specifically by Mycobacterium kansasii, are not commonly encountered, presenting a challenge for both diagnosis and treatment due to the lack of an established management protocol in the pediatric population. This situation affects the clinical course and associated complications. In this article, we address the case of an infant with a history of prematurity, Down syndrome, congenital heart disease, hypothyroidism, bronchopulmonary dysplasia, and immunodeficiency secondary to Down syndrome, who presented with pulmonary sepsis with multiple complications and cervical adenitis. A lymph node biopsy revealed the presence of Mycobacterium kansasii. The patient posed a medical challenge for the treating team due to the unexpected progression of the disease and the limited information in the literature regarding specific treatment considering her age, comorbidities, and intrinsic resistance of this mycobacterium to certain medications.
Introduction: Because the clinical manifestations of dengue can range from mild to severe, finding methods to predict severe cases will be helpful in using medical resources efficiently. One potential predictor of severity is the neutrophil to lymphocyte ratio (NLR). Material and method: We conducted a retrospective case-control study of 232 medical records from a hospital in northern Peru to evaluate how NLR may differentiate between cases of dengue with warning signs (DWWS) and cases without warning signs (DNWS). Results: Bivariate analysis revealed that DWWS is associated with a decreased NLR, a history of reinfection, and younger age. The NLR distribution was biphasic for DNWS cases. We identified an NLR threshold of 1,5, which has a sensitivity of 86% for identifying DWWS cases and a specificity of 71% for correctly identifying DNWS cases. Discussion: The biphasic NLR distribution suggests a single threshold is more effective for ruling DNWS out than for identifying cases. Nonetheless, these data suggest that NLR can serve to efficiently screen cases at high risk of developing DWWS and accurately rule out DNWS cases. NLR can be used as a simple diagnostic tool for triaging dengue patients, allowing for the optimized allocation of healthcare resources.
Introduction: Histoplasmosis is an endemic mycosis in Colombia; however, data on its clinical characteristics in the recent era are limited, particularly regarding differences between people living with HIV (PLWH) and those without. This study aimed to describe and compare the characteristics of hospitalized patients with histoplasmosis according to their HIV serological status. Materials and Methods: This retrospective cohort study was conducted at a university hospital in Medell & iacute;n, Colombia. All adult patients (>= 18 years) hospitalized between January 1, 2014, and December 31, 2021, with a confirmed diagnosis of histoplasmosis via culture, histopathology, molecular biology, or urinary antigen detection were included. Demographic, clinical, laboratory, and outcome data were extracted and analyzed. Results: A total of 51 patients with confirmed histoplasmosis were included. The median age was 38 years (IQR 29-46), and 86.3% (n=44) were male. Of the patients, 80.4% (n=41) were PLWH, among whom 97.6% (40/41) had a CD4+ T-cell count <200 cells/& micro;L. None of the HIV-positive patients were receiving active antiretroviral therapy at the time of their diagnosis. Progressive disseminated disease was significantly more frequent among PLWH (92.7% vs. 60.0%; p=0.021). Co-infection with tuberculosis was documented in 25.5% (13/51) of the cohort. Overall, in-hospital mortality was 9.8% (5/51), and was numerically higher in the HIV-negative group (20.0% vs. 7.3%; p=0.250). Discussion: In this referral center, histoplasmosis in hospitalized patients predominantly affected men with advanced, untreated HIV infection. The high frequency of tuberculosis coinfection highlights the critical diagnostic challenges. The observed mortality was lower than that reported in other regional series, which may be related to management in a specialized center, although this finding requires validation in prospective multicenter studies.
El 20 de noviembre de 2025 murió uno de los científicos colombianos más importantes, quien realizó aportes desde la epidemiología y la patología, al entendimiento del desarrollo del cáncer gástrico. El Dr. Pelayo Jesús Correa, nació en el municipio de Sonsón (Antioquia) en 1927 y se graduó como médico en la Universidad de Antioquia en 1949. Luego, se especializó en patología en la Universidad de Emory, en Atlanta, Estados Unidos (1952). Una vez retornó a Colombia, se vinculó como profesor en el Departamento de Patología de la Universidad del Valle (del cual fue su director) y, además, fue decano del Programa de Medicina de la Universidad del Valle. En el año 1962, los doctores Pelayo Correa, Carlos Cuello, Guillermo Llanos y William Haenszel, fundaron el Registro Poblacional de Cáncer de Cali (RPCC), el primer registro basado en la población en América Latina y que continúa siendo una de las principales fuentes de datos epidemiológicos en la re-gión1. Fue científico visitante del Instituto Nacional del Cáncer de Estados Unidos desde 1970 hasta 1973, y posteriormente se vinculó como profesor de Patología en la Universidad Estatal de Luisiana, en New Orleans, donde estuvo vinculado hasta el año 2005, desde donde se trasladó a la Universidad de Vanderbilt en Nashville, debido al daño que sufrió su laboratorio por el paso del huracán Katrina. En Nashville continuó su investigación hasta su retiro, en el año 2015.
ntroduction: Latent tuberculosis infection (LTBI) is characterized by a sustained immune response to Mycobacterium tuberculosis antigens without clinical, radio-graphic, or microbiological evidence of active disease. The aim of this study was to assess medical and social risk factors for progression of latent tuberculosis infection (LTBI) to active pulmonary tuberculosis in children with household TB contact.Materials and methods:A prospective study (2022–2024) involved 120 children aged 4–12 with LTBI. Two groups were formed: children who developed active TB (LTBI-to-TB group) and those who remained latent. Inclusion required consent. Clinical, immunological (IL-2, IgM), and social factors were analyzed. Risk was evaluated via relative risk (RR) and 95% confidence intervals.Results: Progression to active TB was linked to bacillary TB contact (RR=6.5), maternal TB (RR=5.2), anemia (RR=3.8), ≥5 ARI episodes/year (RR=26.0), ineffective BCG (RR=3.0), single-parent homes (RR=11.0), territorial TB (RR=4.5), helminthiasis (RR=10.0), chickenpox (RR=5.0), genitourinary diseases (RR=8.0), ENT pathology (RR=5.7), isoniazid monotherapy (RR=2.7), COVID-19 or HIV exposure (RR=8.0), IL-2 ≥311.1 ng/ml, and IgM ≥1.18 IU/ml.Discussions: Immunological and social factors increase LTBI progression risk. High-risk children need tailored monitoring.
Introducción:Las infecciones por microorganismos multirresistentes han aumentado en la población neonatal, por lo cual se han desarrollado estrategias para su control y prevención como el tamizaje rectal de microorganismos multirresistentes. El objetivo de este estudio fue determinar la prevalencia de colonización por bacterias multirresistentes y su asociación con factores clínicos en pacientes remitidos o con hospitalización previa en la URN, así como la incidencia de infecciones asociadas a la atención en salud causadas por estos microorganismos.Materiales y métodos:Estudio analítico de cohorte retrospectivo de pacientes ingresados a la unidad de recién nacidos del Hospital Universitario San Ignacio en Bogotá, entre el 2016 y el 2020, cuyo criterio de inclusión era ser remitidos de otra institución o que hubieran cursado con hospitalización previa y se les realizó hisopado rectal. Para el primer objetivo se determinó la prevalencia de colonización y la incidencia de infección asociada al cuidado de la salud por bacterias multirresistentes según la condición de colonización y para el segundo objetivo las variables independien-tes fueron: condiciones preexistentes, uso de antimicrobianos, procedimientos invasivos, accesos centrales y el tiempo de hospitalización previa y la dependiente fue la colonización por bacterias multirresistentes. Se realizó un análisis descriptivo de las variables para el primer objetivo y para el análisis exploratorio de factores asociados a la colonización se empleó una regresión logística. Resultados:722 pacientes incluidos, 68 presentaron hisopados positivos (9,42 %) en una ventana de observación de 40 meses. El principal aislamiento en los hisopados e infecciones asocia-das a la atención en salud (IAAS) fueron las enterobacterias productoras de betalactamasas de espectro extendido diferentes a E. coli. La incidencia de IAAS por microorganismos multirresis-tentes fue de 2,94 %. Se encontró asociación entre el uso de antibióticos previos OR 2,15 (IC 1,14 – 4,05), asfixia OR 0,07 (IC 95 % (0,01 - 0,56) con el hisopado positivo.Discusión:Este estudio permitió identificar una prevalencia importante de colonización de bacterias multirresistentes en los recién nacidos con hospitalizaciones previas o remitidos a la URN del hospital mediante elprotocolo de hisopado rectal. Las infecciones asociadas a la atención en salud fueron más frecuentes en los pacientes colonizados por estas bacterias lo que resalta la importancia de la tamización en este grupo poblacional ya que permite tomar acciones en la atención para prevenir infecciones cruzadas. Los resultados de este estudio demuestran la especial relevancia de la tamización en pacientes con antecedente de uso de antibiótico.
Introducción: El manejo de personas que viven con VIH ha experimentado avances significativos en las últimas décadas con el tratamiento antirretroviral (TAR); sin embargo, los profesionales de la salud continúan enfrentando desafíos en el manejo clínico, particularmente en lo que respecta a la interpretación de la carga viral y la toma de decisiones terapéuticas. El objetivo de este estudio fue unificar definiciones y directrices para la interpretación y manejo de las viremias de bajo nivel persistentes en personas con VIH que reciben terapia antirretroviral en Colombia.Materiales y métodos: Revisión sistemática de la literatura en torno a cuatro preguntas clínicas. La evidencia se clasificó según los niveles del Oxford Centre for Evidence-Based Medicine (2009). Se empleó un consenso Delphi modificado con expertos nacionales. El umbral de consenso fuerte se definió como el acuerdo de al menos el 80% de los expertos participantes.Resultados: Se revisaron dieciocho documentos relevantes. Se desarrollaron doce recomendaciones con consenso fuerte, enfocadas en la definición de viremias de bajo nivel persistente, el uso dirigido de genotipificación a partir de 200 copias/mL y la transición a esquemas de alta barrera genética.Discusión: Estudios recientes sugieren que la viremia de bajo nivel persistentemente detectable puede aumentar el riesgo de resistencia emergente y de fracaso virológico. Este documento adopta un enfoque más conservador y clínico, en línea con las recomendaciones del DHHS y de GeSIDA. Se enfatiza la importancia de evaluar la adherencia, optimi-zar los esquemas y usar la genotipificación de manera juiciosa. En conclusión, la evaluación cuidadosa de la adherencia, la utilización de esquemas de alta barrera genética y un enfoque individualizado permiten optimizar la atención de las personas con VIH con viremias de bajo nivel persistentes, especialmente en escenarios clínicos de incertidumbre.
Introduction: Hemotropic microorganisms, such as Trypanosoma spp., affect many animals, including water buffaloes. Some developing countries have reported trypanocide resistance to Diminazene Aceturate. This study aimed to describe the presence of resistance associated with trypanocide treatment in Colombian water buffaloes.Materials and methods: In this cross-sectional study, 202 individuals were sampled from an endemic area in Colombia. The molecular technique quantitative po-lymerase chain reaction (qPCR) was performed targeting the 18S gene for Trypanosoma spp., and phylogenetic analysis based on the ITS1 region was performed.Results: Sixty-one animals (30.2%) were positive for Trypanosoma spp. and fifty-two sequences were completely analyzed. Of the sequences, 76.9% (40/ 52) were compatible with T. vivax and 23.1% (12/ 52) with T. theileri; the obtained results did not show homologous sequences for T. evansi. Subsequently, positive animals were treated with Diminazene Aceturate at the recommended dose (7 mg/kg), and a final qPCR was performed one month after the end of the treatment. All animals were negative for Trypanosoma spp. after treatment with the drug.Discussion: Although no resistance to the drug was detected in the animals included in the study, it is necessary to implement continuous surveillance of trypanocidal drug resistance to verify the circulation of resistant strains in Colombia and endemic areas in other countries.
