This study aimed at developing a strategy to gain access to more Astragalus flavonoids and then enzymatically acylated through transesterification with fatty acid vinyl esters by Novozym435. The extraction of total flavonoids was optimized by considering the concentration of ethanol, ultrasonic extraction temperature, liquid to material ratio and ultrasonic power. The optimized conditions were as follows: 20:1(mL/g) of the liquid to the material, 70% ethanol ultrasonic temperature of 80 celcius with the aid of ultrasonic waves in 300 W. Calycosin-7-O-Beta-D-glucoside (C7G) and formononetin-7-O-Beta-D-glucoside (F7G) were separated from total flavonoids by column chromatography and then identified by mass spectrometry (MS) and nuclear magnetic resonance (NMR). Furthermore, the absorption peak at approximate 1700 cm -1 in IR analysis indicated acylation did actually take place.
Background Aim This study aimed to identify the prognostic significance of Fibroblast activation protein alpha (FAPalpha) in solid tumor. Method A literature search was performed by using Pubmed, EMbase and CNKI for relevant articles published with the following key words “fibroblast activation protein” or “seprase”, “clinical” and “cancer” or “carcinoma”. Pooled effects with 95% confidence interval (CI) for outcomes (age, gender, positive ratio, histological differentiation, depth of tumor invasion, lymph node metastasis, clinical stage and overall survival) were calculated. Besides, immunohistochemical analysis was performed to evaluate FAPalpha positive ratio in normal, benign and malignant tumor tissues as well as in different degrees of differentiation, clinical stages and lymph node metastasis tissues. Results Finally, 28 articles with different types of solid tumors were included in the meta-analysis. Results revealed that solid tumors had a higher positive ratio of FAPalpha expression (OR: 39.51, P < 0.001), and FAPalpha overexpression also indicated higher degree of tumor depth invasion (OR: 2.82, P = 0.010), lymph node metastasis (OR: 2.52, P = 0.003), clinical stage (OR: 3.36, P = 0.003) and worse overall survival (HR: 2.03, P < 0.001). Results from immunohistochemistry demonstrated that FAPalpha expressed higher in malignant solid tumors than that in benign solid tumors ( P = 0.022), and it significantly overexpress in lymph node metastasis tissues ( P = 0.04). Conclusion Our findings indicate that FAP? expression is associated with worse prognosis in solid tumors and may be a promising prognosis predictor.
Background Theacrine is a purine alkaloid with sedative, hypnotic and liver protection effects, which was investigated in treatment of insomnia, liver damage and hepatic steatosis. These are generally chronic diseases needing long-term medication. Sustained release preparations have good effect and compliance in prolonged treatment. Aim This study aimed to develop theacrine sustained release tablets using wax matrix material with simple preparing process. Methods: The content assay method of theacrine was developed using UV-visible spectrophotometry, and the method was fully validated. Wax matrix materials were used to prepare theacrine sustained release tablets by melting-granulation tableting process. The formulation was optimized by single-factor experiments with drug release behavior as evaluation index. The parameter f2 was used to compare the similarity of the drug release behavior of theacrine tablets prepared by different formulations. Weight variation and friability was also used to control the quality of theacrine tablets. Results The established UV analysis method had good specificity, linearity and precision. The results of single factor experiments showed that theacrine tablets of good sustained release effect were prepared with glycerol monostearate as matrix material and microcrystalline cellulose (MCC) as filler, and the ratio of glycerol monostearate to MCC was 1:2. The accumulative drug release percentage of theacrine sustained release tablets was 18.33% at 2 h and more than 95% within 12 h. Conclusion In this work, theacrine tablets were successfully prepared with wax matrix material and the tablets exerted a slow complete sustained release effect within 12 h without burst effect. The theacrine sustained release tablets were expected to be used as a long-acting preparation for chronic disease.
Aim The objective of this study was to investigate solvent extracts of Sceletium tortuosum for their neuroprotective effect and further identify the constituents in the pharmacologically active solvent fractions. Methods Sceletium tortuosum material was extracted with petroleum ether and ethyl acetate respectively. The extracts were further subjected to silica-gel column chromatography to obtain six sub-fractions. The sub-factions were tested for neuroprotective effects based on their evaluation in MPP + -injured N2a cells and L-glutamate-injured PC12 cells by MTT assay. The sub-fractions were further tested by ultraperformance liquid chromatography (UPLC) coupled to quadrupole-time-of-flight mass spectrometry (qTOF) with collision energy (MSE) to identify relevant constituents using MassFragment software in Masslynx4 software. Results The highly non-polar petroleum ether fraction and the polar ethyl acetate fraction showed potential in vitro neuroprotective activity. The UPLC-qTOF-MS E results of sub-fractions indicated the presence of dihydrojoubertiamine, mesembrenone, mesembrine, 2-oxomesembrine, Delta 7 -mesembrenone, N-demethyl-N-formylmesembrenone, dihydrobuphanamine acetate, sceletium A4, sceletenone, Delta 7 -N-demethyl-mesembrenone, 2-oxomesembranol, N-methyldihydrojoubertinamine and other unnamed components. Conclusion The polar and nonpolar extracts of Sceletium tortuosum exhibit neuroprotective effect in N2a cells or PC12 cells injured by MPP + or L-glutamate respectively. Two sub-fractions each of the petroleum ether and ethyl acetate fractions were observed to have comparatively higher neuroprotective efficacy than other sub-fractions and the MS profile indicated the presence of dihydrojoubertiamine and an unidentified non-alkaloid in these two sub-fractions. While the presence of mesembrine, mesembrenone and other alkaloids were observed in the other two neuroprotective sub-fractions, the results indicate that there are unidentified constituents of Sceletium tortuosum that require further investigation.
Background Theacrine (1,3,7,9-tetramethyluric acid, TC) is a purine alkaloid rich in the tender leaves of an unusual Chinese tea known as Kucha. TC possessed diverse pharmacological activity including antioxidative, anti-inflammatory, analgesic and anti-depressive effects. Aim In this work, sustained release tablets of TC were intended to be developed with insoluble matrix material to provide a kind of preparation of prolonged action. Methods Direct tableting process and wet granulation tableting process were screened for preparing insoluble matrix tablets of TC. Single factor testes were carried out for the formulation optimization by using drug release behaviour as evaluation index. The parameter f 2 was used to assess the similarity of the drug release behaviour of TC tablets prepared by different formulations. The common quality of TC tablets prepared by optimized formulation was investigated as well. Results With ethyl cellulose (EC) as sustained matrix material, lactose as filler, aerosil as lubricant and anhydrous ethanol as moistening agent, the TC insoluble matrix tablets of sustained release were successfully prepared by wet granulation tableting method. The prepared TC tablets showed no obvious burst effect with about 40% released within 2 h, and TC was almost completely released within 12 h with an accumulative drug release percentage of 84.89%. The results of common examination showed that the TC tablets were in line with the requirements of Chinese Pharmacopoeia. Conclusion TC tablets of good sustained release behaviour were prepared simply by wet granulation tableting process with EC as release control materials. This work provided scientific evidence for development of TC preparation of prolonged action.
Background Core planar cell polarity gene Celsr3 (Cadherin EGF Laminin Seven Pass Receptor), the mammalian orthologs of Drosophila Flamingo/starry night , encode proteins of the cadherin superfamily. Celsr3 plays a critical role in forebrain wiring, particularly the development of anterior commissure (AC) and the internal capsule (IC). Previous work showed that inactivation of Celsr3 in hippocampal projection neurons perturbed intrinsic hippocampal wiring and mutant mice showed impaired learning and memory. However, the effects of Celsr3 inactivation on hippocampal efferent fibers, synaptogenesis and adult neurogenesis are still not clear. Method We studied Emx1-Cre ; Celsr3f/- (abbreviated as Celsr3 | Emx1 ) mutant mice as the experimental group, processed histological staining and electron microscopy analysis. Result Inactivation of Celsr3 in hippocampal projection neurons impaired efferent fiber projections, whereas afferent fibers from entorhinal cortex were unaffected. In addition, it resulted in incomplete maturation of pyramidal cell dendrites and synaptogenesis, and increased adult neurogenesis in the dentate gyrus. Conclusion Hippocampal efferent pathways and synapse formation are defective in Celsr3 | Emx1 mutant mice, while adult neurogenesis in the dentate gyrus is increased.
Aim We want to increase the biological stability of short peptides by PEG modification. Methods Through connecting the maleimide group to one end of polyethylene glycol and adding a cysteine (Cys) to one end of the short peptide, the short peptide was finally modified by PEG through chemical bonds. We established a reverse high-performance liquid chromatography (RP-HPLC) detection method to detect the change of substances before and after the reaction; screened out the optimal detection method by orthogonal test; purified the modified short peptide by ultrafiltration; detected the reaction by infrared spectroscopy Changes in functional groups; tested the stability of RP-1 in rat plasma before and after modification. Result and discussion Through single factor test and orthogonal test, the pH in the reaction was 6, reaction temperature was 25 ?C, reaction time was 1H, and reaction ratio was PEG: RP-1C 1:1.5. The solution does not contain RP-1C after ultrafiltration. Peripheral plasma stability testing found that the modified short peptides greatly enhanced the stability. Conclusion Through experiments, we found the best conditions for the modification of short peptides, purification methods, and the stability of the modified short peptides was greatly improved.
Background METTL3 (methyltransferase like 3) and FTO (fat mass and obesity-associated protein) are critical for establishing and regulating the m6A (N6-methyladenosine) modification, but very little is known about the function of m6A in the immune system or its role in interactions within the host immune system. Methods Western blotting experiment was used to check the expression of the host proteins related to m6A modification after H7N9 influenza virus infection. Immunofluorescence experiment was used to identify whether the subcellular localization of related proteins. Moreover, we knocked down and over express endogenous METTL3 or FTO in A549 cells, and then infected the cells with H7N9 to test whether methyltransferases or demethylases affect H7N9 replication. Result We confirmed that the expression pattern of m6A proteins was altered during H7N9 infection. METTL3 and FTO were located in the nucleus, and YTHDF3 was located in the cytoplasm. Furthermore, our results demonstrate that downregulation of METTL3 or FTO expression in A549 cells severely impairs viral protein expression and H7N9 infection. Conclusions METTL3 and FTO are critical for H7N9 replication which may represent a new mechanism for the control of H7N9 replication and host-pathogen interactions.
Background Tinibs were a kind of important epidermal growth factor receptor (EGFR) inhibitors used as potential therapeutic agents in treating non-small cell lung cancer (NSCLC) in clinic. The drug resistance of clinical used tinibs made the development of more active tinib analogues an attractive field in research. Quinazoline ring was regarded as the key fragment in tinibs and quinazolinone was indispensible intermediate in the synthesis of quinazoline. Thus, synthesis of quinazolinone intermediates was a key step which would further limit the overall yield of final product of tinib analogues. However, the commonly used synthetic scheme was somewhat complicated and time consuming with relatively low yield in heterocylization of quinazolinone and its derivatives. Aim In this work, we intended to explore an effective way to improve synthesis of heterocyclization of 6,7-bis(2-methoxyethoxy)quinazolin-4(3H)-one (compound 5), the key fragment of erlotinib analogues, in both reaction procedure and yield, thus to provide reference to synthesis of other quinazolinone derivatives. Methods A simple microwave-assisted one-pot reaction was employed to improve the synthesis of heterocyclization of quinazolinone ring. The reaction conditions, including microwave power, temperature and time of reaction, were screened to achieve high yield under simple operation. Results 6,7-bis(2-methoxyethoxy)quinazolin-4(3H)-one (compound 5) was successfully synthesized from starting material of 4,5-bis(2-methoxyethoxy)-2-nitrobenzonitrile by microwave reaction, which was finished in 1 hour just by one step. The yield of heterocyclization was increased from 29.8% of commonly used three-step scheme to 50% herein. Conclusion Microwave reaction efficiently improved synthesis of heterocyclization of 6,7-bis(2-methoxyethoxy)quinazolin-4(3H)-one into "quinazolinone in both synthetic procedure and yield. The results may provide valuable reference to synthesis of other quinazolinone derivatives.
Sceletium tortuosum is a well-known medicinal plant in South Africa with potential applications. Its raw material, extracts and isolated alkaloids are used as dietary supplements, natural medicines and health food. In this paper, methods of planting, extraction, isolation and identification of Sceletium tortuosum , as well as its chemical structure of main extracted alkaloids, their related pharmacological effects and mechanisms for treating the disease are reviewed. In general, Sceletium tortuosum is active to central nervous system (CNS) by inhibiting phosphodiesterase isozyme 4 (PDE4), serotonin (5-HT) uptake and acetylcholinesterase (AChE). It also acts as a monoamine releasing agent for antidepressant effects. Therefore, it is a useful therapeutic agent in clinical use.
Background To explore the pharmacodynamic evaluation and mechanism research of BOC26P against breast cancer, and to provide a basis for the treatment of breast cancer. Method MTT assay was used to detect the cytotoxicity of BOC26P against 4 breast cancer cell lines (MCF-7/TAX, MDA-MB-231/PT, MDA-MB-231and MCF-7), and as well as the non-tumor cell lines MCF-10A, in various drug concentrations (from 0.004 to 1uM). Western Blotting and Real-Time PCR assay were used to detect the relative protein and gene expression level after treatment with BOC26P in MCF-7/TAX. The effect of BOC26P on Specific fluorescent P-gp substrate accumulation in MCF-7/TAX was analyzed by flow cytometry; Molecular docking was used to analyze the binding capacity between BOC26P, Cyclosporine A, and Verapamil. FCM assay staining with Annexin V-FITC/PI and Propidium iodide was used to measure the apoptosis and the cell cycle after treatment with BOC26P in MCF-7/TAX, MDA-MB-231/PT, MDA-MB-231, and MCF-7; Detection of mitochondrial membrane potential after treatment with BOC26P inMCF-7/TAX, MDA-MB-231/PT, MDA-MB-231and MCF-7; Western Blotting and Real-Time PCR assay was used to detect the apoptosis relative protein and gene expression level after treatment with BOC26P in MDA-MB-231, MCF-7, MDA-MB-231/PT, and MCF-7/ADR. Results Cytotoxicity assay showed that BOC26P could effectively suppress 4 breast cancer cell lines (MCF-7/TAX, MDA-MB-231/PT, MDA-MB-231, and MCF-7) with an IC50 value of under 0.5 ?M. The IC50 value of BOC26P on non-tumor cells MCF-10A was 32.29 uM. The binding ability of BOC26P to P-gp in breast cancer cells was weak. There was no significant effect on the intracellular accumulation of Rhodamin 123(Rh123), P-gp binding specific fluorescence substrate, and multi-drug resistance protein P-gp expression in MCF-7/ADR and MDA-MB-231/PT tumor cells; BOC26P induced MCF-7/TAX, MDA-MB-231/PT, MDA-MB-231 and MCF-7 cells cycle arrest at G2/M phase and lead to cell apoptosis. BOC26P induced significant activation of p53 protein in MCF-7/ADR and MAD-MB-231/TAX cells. Under the same conditions, BOC26P promoted Bax expression while inhibited Bcl-2 expression, and could significantly cause activation of Cleveland PARP and Clevead Caspase3. The results demonstrated that BOC26P may induce apoptosis through the death receptor apoptosis pathway. Conclusion It is known that BOC26P has a significant proliferation inhibitory effect on breast cancer cells without serious side effects. BOC26P has the Potential to be developed into a clinical substitute drug for triple-negative breast cancer and drug- resistance breast cancer. BOC26P, a cytotoxic antitumor candidate compound but not a P-gp substrate, does not interfere with P-gp expression and efflux function in the P-gp-positive drug-resistant breast cancer cells, that is, it can avoid P-gp mediated multidrug resistance pathway in breast cancer. BOC26P activates the membrane stability protein Bcl-2 family in MDA-MB-231/PT, MCF-7/ADR and MA-MB-231 mitochondrial pathways of drug-resistant human breast cancer cells and their maternally sensitive cells, causing mitochondrial rupture, releasing P53, and activating the caspase-mediated apoptosis pathway, thus causing apoptosis of breast cancer cells. The significant inhibitory activity of BOC26P on breast cancer resistant cells may be related to P53, Activation of P53 and other tumor suppressor genes alters the expression of Bcl-2 and Bax, further activates the Caspase cascade initiates apoptosis pathway, and cause apoptosis eventually.
Core tip: RP1 can bind to RAGE specifically in vitro . RP1 can effectively alleviate the destroy of TJs of blood-brain barrier and the decrease of permeability of BBB caused by Amyloid beta . RP1 can competitively inhibited the interaction of Amyloid beta with the RAGE in vitro, and effectively inhibit Amyloid beta transport into the brain. Abstract: Background The interaction of the receptor for advanced glycation end product (RAGE) on blood-brain-barrier (BBB) with Amyloid beta , plays an important role in the occurrence and development of AD. RP1 is a RAGE specific binding peptide, which was discovered in our previous experiments, and it has been proved to be effective on AD cell model, however, its effects on BBB tight junctions (TJs) and on Amyloid beta transport into the brain is unclear. Methods Immunofluorescence experiment was used to identify whether RP1 bound with RAGE specifically. BEnd3-immortalized mouse brain microvascular endothelial cells were used to construct a BBB model. TEER and FD40 tests were used to confirm the stability of the BBB model, and the colocalization of the RP1 and RAGE on the surface bEnd3 cells was observed with confocal microscopy. Results We confirmed that RP1 can bind to RAGE specifically in vitro . Functional analyses indicated that RP1 can effectively alleviate the destroy of TJs of BBB and the decrease of permeability of BBB caused by Amyloid beta . Furthermore, RP1 can competitively inhibit the interaction of Amyloid beta with the RAGE in vitro, and effectively inhibit Amyloid beta transport into the brain. Conclusion RP1 can inhibit BBB damage induced by Amyloid beta and block RAGE- Amyloid beta interaction effectively, and RP1 can be a candidate of RAGE inhibitors contributing to AD treatment.
Background OC26 and its pro-drug BOC26P, both ortho-aryl chalcone compounds, showed a well-defined antitumor activity in various cancer cells especially in drug-resistant tumor cell lines. Aim The purpose of this study was to investigate the bile excretion characteristics of OC26 after OC26 and BOC26P administered in rats respectively. Method An ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for OC26 in rat bile. Liquid-liquid extraction with ethyl acetate method was use to pretreat the bile samples. After that, a gradient mobile phase at a flow rate 0.5mL/min of acetonitrile and 2mM CH 3 COONH 4 with 0.1% aqueous ammonia solution (v/v) and the positive ion alternate mode separated and quantified OC26. Bile samples were collected from rats after intravenous injection (i.v) 12.5mg/kg of OC26 and BOC26P, respectively. Results For method validation, the method showed high extraction recovery. The assay showed a good linearity with correlation coefficient >0.99 at the concentration ranges of 20-2000ng/mL. All data were within the required limits. The bile excretion results showed that the excretion amount of OC26 was gradually stabilized after 2h. The accumulative excretion percentage of OC26 after i.v 12.5mg/kg BOC26P was significantly higher than that of OC26 after i.v 12.5mg/kg OC26. Significant gender differences were also observed in bile excretion of OC26. Conclusion This method was selective, sensitive and reliable and successfully applied to the bile excretion of OC26. This study provided theoretical basis for OC26 further research.
Carbon monoxide (CO), released in industrial gases and released by the burning of fuels such as coal, increases its concentration in nature as an environmental pollutant with the advancing industry and threatens human health. However, in the natural balance of life, carboxidotrophic microorganisms use CO as a carbon and energy source with their carbon monoxide dehydrogenase (CODH) enzymes. These microorganisms, which show life forms in both aerobic and anaerobic conditions, catalyze the conversion of highly toxic CO to CO2 (carbon dioxide) with various CODH enzymes with very different cofactors. Thus, they have significant potential impact in the creation of energy-rich compounds and the incorporation of carbon into the environment in the global carbon cycle. It offers great potential for biotechnology due to the enzyme catalyzed reaction. With designs using CO dehydrogenase, CO-based probes or fuel cells can be produced in biosensors. In this review, it is aimed to present information about the industrially important CO dehydrogenases.
This study was carried out to determine some important pomological and biochemical characteristics of strawberry tree genotypes selected in Bolu province in Turkey. Twenty genotypes, which were found to be most promising according to the data obtained during the two years in the study, were examined. Fruit length was changed between 15.48-21.44 mm, fruit width was 14.07-21.46 mm and fruit weight was between 2.94-7.47 g in the examined genotypes. Soluble solid contents (SSC) was changed 13.95-21.75%, fruit juice pH value 3.40-3.83, titratable acid (TA) 0.48-0.83%; ash content 0.428-0.848%, moisture content 63.78-77.93%, nitrogen content 0.110-0.300% and protein content 0.760-1.850% respectively. The results obtained suggest that the comparison of that results of the studies done in our country and the international data shows that the genetically domesticated genotypes that have grown in the region constitute a promising potential and that selection studies should be continued under controlled conditions.
Background: The atypical cadherin Celsr3, which belongs to the core planar cell polarity group, orchestrates axonal guidance and network wiring. Previous work using regional inactivation of Celsr3 in forebrain showed that Celsr3 is widely involved in hippocampal maturation and connectivity. However, inactivation in the whole forebrain does not provide sufficient specificity to address the function of Celsr3 in detail. Method: We studied the Celsr3|Emx1 mouse mutant model, in which Celsr3 is selectively inactivated in hippocampal projection neurons, but not in entorhinal cortex, basal ganglia and interneurons. Result: In that mutant, the hippocampal cytoarchitecture was almost normal. Inactivation of Celsr3 in projection neurons perturbed intrinsic hippocampal wiring. Consistent with wiring defects, Celsr3|Emx1 mutant mice showed impaired learning and memory, and were less anxiety-prone than control mice. Conclusion: Celsr3|Emx1 mutant mice provide a simple way to study the consequences of defective hippocampal wiring in absence of drastic structural anomalies.
Background Colorectal cancer (CRC) is the most common malignant tumor of digestive system. The metastases is the main cause of mortality in CRC patients, of whom the initial diagnosis is about 25%. In our study, we aimed to identify potential gene biomarkers based on RNA sequencing data to predict and improve CRC patient survival. Method In this study, by screening differentially expressed genes of colon cancer related to liver metastasis, a survival prognostic risk model was constructed by bioinformatics analysis. Here, we conducted our data mining analysis for CRC by integrating the differentially expressed genes acquired from Gene Expression Omnibus (GEO) database by primary tumor versus liver metastasis (GSE81582?GSE41258?GSE49355?GSE68468) into The Cancer Genome Atlas (TCGA) database which includes 415 primary tumor and 132 liver metastasis tissue. At the same time, we used transwell, RT-PCR and western to examine the effects of CLCA1 and SPINK4 on the migration of colorectal cancer cells at the cell level. Results We identified intersections of 197 genes (117 up-regulated and 80 down-regulated) between GEO data and TCGA data. Differentially expressed genes in TCGA-COAD by single factor cox analysis, lasso cycle training and multifactor cox analysis composed a survival prognosis prediction model consisted of 7 genes ORM1, CLCA1, C8B, SPINK4, ALDOB, GAMT, C8G. And results of transwell experiments showed that high expression of CLCA1 and SPINK4 can inhibit the migration ability of colon cancer cells LOVO and SW620, meanwhile western blotting showed that the high expression of both genes can upregulate the expression of epithelial phenotypic marker E-cadherin, and Vimentin expression is down-regulated. Conclusion In this study, 197 differentially expressed genes were selected and a relatively robust survival prognosis prediction model was constructed. The model consisted of seven genes: GAMT, C8G, ORM1, CLCA1, C8B, SPINK4, and ALDOB. At the same time, we found that CLCA1 and SPINK4 are closely related to survival prognosis. The predictive model nomogram will enable patients with CRC to be more accurately managed in trials testing new drugs and in clinical practice.
Objective To investigate the clinical effect of levosimendan in perioperative aortic and/or mitral valve replacement. Methods Patients undergoing open heart aortic and/or mitral valve replacement in our hospital from January 2018 to December 2019 were enrolled. 45 patients in the control group received routine perioperative treatment based on dopamine, while 45 patients in the research group received continuous perioperative administration of levosimendan injection for 24h on the basis of routine treatment. The left ventricular ejection fraction (LVEF), left ventricular end-diastolic diameter (LVDd) and left ventricular end-systolic diameter (LVDs) were evaluated by color doppler echocardiography before and one week after surgery. Postoperative mechanical ventilation weaning time, length of ICU stays, number of vasoactive drugs used and withdrawal time; indexes of liver and kidney function before and on the day after surgery to 10 days after surgery; use of in vitro support techniques such as aortic balloon pulsation (IABP), continuous renal replacement therapy (CRRT) and extracorporeal membrane oxygenation (ECMO) within 5 days of perioperative period. Results The improvement of LVDs and LVEF in the study group using levosimendan one week after the operation was significantly better than that in the control group (P value was 0.013 and 0.001, respectively), and fewer kinds of vasoactive drugs were needed (P<0.001), and the risk of postoperative AKI in the study group was significantly lower than that in the control group ( P=0.047 ). Conclusion The perioperative use of levosimendan can effectively promote the recovery of cardiac systolic function and reduce the risk of postoperative AKI.
