AbstractHepatocellular carcinoma (HCC) is the sixth most common malignancy worldwide and the third most common cause of death from cancer, after lung and stomach cancer. Hepatitis B virus (HBV) infection is closely related to HCC and is a major cause of HCC. HBV is a lysogenic virus of the hepadnavirus family. Its genome presents a slack, ring-like, double-chain structure, containing four open reading frames. The X region encodes the product HBV X protein (HBx), which is a multifunctional regulatory protein that plays an important role in intracellular signal transduction, viral genome replication and transcription, cell proliferation and apoptosis, cell cycle progression, protein degradation, and genetic stability of hepatocytes. This article summarizes the recent research on the mechanism of promotion of initiation and progression of HCC by HBx protein.
Hepatitis C virus（HCV） is the main pathogen causing chronic hepatitis and primary liver cancer. Various viral proteins and host cell molecules are involved in the HCV cell entry, but the mechanism of infection has not been completely elucidated. The transferrin receptor can act as a receptor for many viruses during cell entry. The transferrin receptor is not only closely related to HCV-induced iron metabolism disorders but also mediates the fusion of HCV with the host cell membrane as a specific receptor for CD81-dependent viral adhesion.
Abstract The purpose of this study was to study the association of deep fungal infection with glucose levels in critically ill intensive care unit (ICU) patients. Fasting blood glucose level was measured for 108 critically ill ICU patients in the morning. After analyzed according to the Spearman method found deep fungal infections in patients with the rise in blood glucose levels and the ratio increases, a positive correlation between the two. Deep infection in critically ill ICU patients and their blood glucose levels was closely related, and therefore, there should a focus on controlling blood sugar levels in patients.
AbstractHuman microecology has been extensively investigated. Similar to an important physiologically functioning organ of the human body, the microecological system is one of the leading systems for environmental survival, health, genetics, disease, and aging. It is also an essential carrier for drug metabolism and microbial resistance. The occurrence, development, and deterioration of many infectious diseases are closely related to human microecological systems. This study mainly focuses on the changes in microbial groups associated with various infectious diseases to explore the relevant role of human microecology in the development of infectious diseases and its breakthrough implications in future accurate treatments of infectious diseases.
AbstractHPV is classified into high-risk and low-risk types depending on its probability of leading to tumorigenesis. Many studies have shown that HPV infection, especially the infection caused by the high-risk type, is always related to prostate cancer, bladder cancer, penile cancer, testicular cancer, and other urinary system tumors. However, previous studies differed in sexual openness and racial genetic susceptibility of the study object, sample size, and experimental methods. Hence, the correlation between high-risk HPV infection and urinary system tumors remains controversial. The early open reading frame of the HPV genome is composed of E1–E7, among which E6 and E7 are the key transfer proteins. The combination of these proteins with oncogene and anti-oncogene may be one of the mechanisms leading to tumorigenesis.
AbstractAutoantibodies (AAbs) against von Willebrand factor (vWF)-cleaving protease ADAMTS13 causally relate to thrombotic thrombocytopenic purpura (TTP). How anti-ADAMTS13 AAbs are generated is unknown. Starting from reports according to which influenza infection can trigger TTP by the production of ADAMTS13 AAbs, this study explores influenza viruses and ADAMTS13 protein for common peptide sequences that might underlie anti-influenza immune responses able to cross-react with ADAMTS13. Results document that numerous peptides are shared between influenza A and B viruses and ADAMTS13, thus supporting the hypothesis of cross-reactivity as a mechanism driving the generation of anti-ADAMTS13 AAbs.
Abstract Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is an acute progressive respiratory failure caused by severe infection, trauma, shock, poisoning, inhaled harmful gas, acute pancreatitis, and pathological obstetrics. ALI and ARDS demonstrate similar pathophysiological changes. The severe stage of ALI is defined as ARDS. At present, a significant progress has been achieved in the study of the pathogenesis and pathophysiology of ALI/ARDS. Whether or not ALI/ARDS patients can recover depends on the degree of lung injury, extra-pulmonary organ damage, original primary disease of a patient, and adequacy in supportive care. Conservative infusion strategies and protective lung ventilation reduce ARDS disability and mortality. In this study, the pathogenesis of ALI/ARDS, lung injury, molecular mechanisms of lung repair, and conservative infusion strategies and pulmonary protective ventilation are reviewed comprehensively.
Abstract A liver fluke is a digenetic trematode parasitizing in the hepatic ducts of human beings or animals. Patients with liver fluke infection suffer from a series of hepatobiliary diseases. The prevalence of cholangiocarcinoma is significantly high in areas with a high incidence of clonorchiasis. A liver fluke is an important biocarcinogenic factor in the occurrence of cholangiocarcinoma. The secretory products of the body of this parasite and long-term mechanical stimulation induce continuous inflammation of the bile duct. Gene expression of the bile duct cells is imbalanced, leading to carcinogenesis of the bile duct. This article provides a summary of recent studies on the epidemiology, clinicopathology, and molecular biology of cholangiocarcinoma induced by liver fluke infection.
Abstract Immune tolerance is a specific lack or negative response of T and B lymphocytes to antigen. According to different formation periods, immune tolerance can be divided into central and peripheral tolerances. The immune tolerance of the body to hepatitis B virus (HBV) after infection is the main cause of chronic HBV infection. In this paper, the functional defects of hepatitis B virus e antigen and dendritic cells, hyporesponsiveness of cytotoxic T lymphocyte, variation of helper T lymphocytes and cytokines, HBV genotype and genome, and the role of host gene polymorphism in the formation of immune tolerance in chronic HBV infection and its related research progress are introduced briefly.
Abstract External abdominal hernia is a common clinical disease. The application of hernia patch is a breakthrough in the treatment of external abdominal hernia. However, complications such as patch infection need to be solved urgently. Patch infection markedly prolongs the hospitalization time and increases the medical expenses of patients. At present, a standard method for the diagnosis, treatment, and prevention of patch infection remains to be developed. This paper summarizes the literature in recent years to explore the research progress in the prevention and treatment of patch infection.
AbstractMycoplasma pneumoniae(MP) is an important pathogen of community-acquired pneumonia in children. As a type of self-limited disease, most MP infections cause mild clinical symptoms, but they can also lead to severe pneumonia or extrapulmonary complications. The resistance rate of MP has increased in recent years. Early and rapid diagnosis of MP infection is important for the treatment and prognosis of the disease. Current methods for diagnosing MP infection include isolation culture, serological diagnosis, and molecular biological diagnosis. This review summarizes the recent research progress in the internal and external laboratory diagnoses of MP infection both at home and abroad and the advantages and disadvantages of various diagnostic methods.
Helicobacter pylori (Hp) is one of the most common human pathogens. The infection caused by this bacterium is closely related to various clinical diseases. However, the eradication rate of conventional quadruple therapy has decreased, but the recurrence rate of infections has increased. The oral cavity is another major storage of Hp in addition to the stomach, and a certain homology exists between Hp in the oral cavity and Hp in the stomach. Periodontal treatment has a certain role in the killing of oral Hp, which can increase the eradication rate and reduce the recurrence rate of Hp in the stomach. This article summarizes the influence of oral Hp on the eradication rate and recurrence rate of gastric Hp.
Abstract Liver transplant is considered the best choice for treating various end-stage liver diseases either at home or abroad. Among patients of liver transplant complicated with tuberculosis (TB), the incidence and mortality of postoperative active TB are bound to increase remarkably. Diagnosing and treating TB in patients with end-stage liver diseases who received immunosuppressants after liver transplant are difficult because of the absence of specific clinical manifestations while being complicated with TB, reduced sensitivity to cellular immunoassay, and interaction between anti-TB drugs and immunosuppressants. Therefore, the screening of high-risk groups, improvement in diagnostic accuracy, preoperative treatment, and reduced interaction between anti-TB drugs and immunosuppressants can help optimize diagnosis and treatment regimes and thus further improve the prognosis of patients.
Abstract This study investigates the hypothesis that cross-reactions may occur between human cardiac proteins and influenza antigens, thus possibly representing the molecular mechanism underlying influenzaassociated sudden unexpected death (SUD). Using titin protein as a research model, data were obtained on (1) the occurrence of the titin octapeptide AELLVLLE or its mimic AELLVALE in influenza A virus hemagglutinin (HA) sequences; (2) the immunological potential of AELLVLLE and its mimic AELLVALE; (3) the possible role of the flanking amino acid aa) context of the two octapeptide determinants in eliciting cross-reactivity between the human cardiac titin protein and HA antigens.
Abstract Patients with diabetes are prone to concurrent infection. The mechanism of concurrent infection is related to factors such as hyperglycemia and weakened defense function. The infections of patients with diabetes include general and special infections. General infection includes infections in the respiratory system, urinary system, hepatobiliary system, and skin mucosa. Meanwhile, special infection includes invasive otitis externa, nasal mucormycosis, necrotizing fasciitis, and emphysema infection. Patients with special infections also have a higher mortality rate than those with general ones. Complicated infection with diabetes is difficult to treat and has poor prognosis. Therefore, a patient requires active treatment once infected with this infection.
The harms of seasonal flu and global pandemic influenza have generally attracted attention. However, the currently administered influenza drugs and flu vaccines have certain limitations. Since the discovery of the small interfering RNA（siRNA） and its mediated RNA interference process, this molecule has been widely used in the study of anti-influenza viral infections because of its high specificity and strong selectivity. The results provided new concepts for the prevention and treatment of influenza virus. However, the siRNA still faces an enormous challenge despite extensive studies on this molecule. The research progress of siRNA in anti-influenza viral infection was reviewed in this study.
Objective: The objective of this study was to understand the pathological mechanism and therapeutic progress in the study of urinary tract infections to provide references for clinical diagnosis and identification and development of therapeutic drugs.Methods: We summarized the types, pathological mechanisms, and therapeutic drugs for urinary tract infections on the basis of recent publications on these infections, both domestic and abroad.Results and conclusions: Urinary tract infection is mainly caused by pathogenic bacterial infection and treated by targeting bacterial adhesion, bacterial toxin, protease, urease, and siderophores, as well as using pili as vaccines and small-molecule drugs. Vaccines that target bacterial adhesion can block well the interaction between pathogens and the body, thereby reducing the incidence of urinary tract infections. The clinical efficacy of vaccines targeting bacterial toxins and proteases needs further evaluation. Vaccines targeting iron carriers retard disease progression and attenuate bacterial colonization. Urease-targeted small-molecule drugs exhibit certain curative effects and serious side effects. Small pili-targeted drugs can prevent and treat urinary tract infections by blocking the colonization and invasion of pathogens in animal models of urinary tract infections on the bladder. Adhesive FimH antibodies have entered Phase Ⅰ clinical trials. However, pilicides, mannosides, and vaccines that target pili, iron carriers, and other virulence factors are still in the experimental or preclinical stages of research.
Sepsis is a systemic inflammatory response caused by infection and a critical illness in pediatrics. This disease is the leading cause of death in infants and children worldwide. An early, appropriate, and adequate anti-infective treatment can effectively prevent disease progression and improve the survival rate of children. However, antimicrobial drug abuse, increased drug-resistant bacteria, and lack of epidemiological data have hampered the effective and rational anti-infective treatment of patients with sepsis and enhancement of the success rate of rescue, especially for children. This article briefly reviews the recent advances in anti-infective treatment for sepsis in children at home and abroad based on sepsis definition, pathogen distribution and drug resistance, infection source control, and rational anti-infection. The results provide a foundation for clinical treatment of sepsis.
In recent years, heavy drug resistance in bacteria has become increasingly serious, and the progress of research and development of new antibiotics are slow. Therefore, seeking an alternative from the safe and effective "old medicine" is a strategy for clinical action against infection. Among them, fosfomycin is extremely relevant. Based on the related literature at home and abroad, this review briefly introduces fosfomycin in the context of progress in synthetic methods, pharmacokinetic and pharmacodynamic characteristics, and antibacterial activities, to provide references for clinical rational use.
AbstractThe study aimed to investigate the correlation between concentration of inhaled arsenic trioxide and dynamic changes in hematotoxicity in rats. Wistar rats were randomly divided into four study groups that were treated with saline (control) or arsenic trioxide at a low (0.1 mg/mL), medium (1 mg/mL), or high (10 mg/mL) dose by intratracheal instillation. Blood samples were collected for analysis at 6, 12, 24, 48, and 72 h after exposure. Compared with the control group, intratracheal instillation of arsenic trioxide affected hematopoietic differentiation in rats, leading to blood cell changes that were related to observation time and concentration.
