BACKGROUND:To investigate the relationship among human papillomavirus (HPV)16 infection and the expression of telomerase catalytic protein subunit (hTERT), tumor suppressor gene p21waf1, proliferation antigen Ki67 in cervical intraepithelial neoplasias (CIN) and squamous cell carcinomas (SCC) of cervix uteri and their significance. METHODS:Tissue microarray combined with in situ hybridization (ISH) and immunohistochemical staining (EliVision plus method) was used to detect the expression of HPV16 RNA, hTERT, Ki67 and p21waf1 proteins in the cervix uteri specimens from 130 subjects, including normal cervical tissue (n=26), CIN (n=46) and SCC (n=58). RESULTS:(1) The positive rate of HPV16 hybridization signals and expression of hTERT, Ki67 in CINII-III, in situ carcinoma and invasive SCC were all significantly higher than in normal cervical tissue (P < 0.05 for all), and was also higher in SCC than in CIN (P < 0.05 for all). There was a significant difference among CIN I, II and III (P < 0.05 for all). (2) The positive expression of p21waf1 protein only in SCC was significantly lower than in normal cervical tissue (P < 0.05), but there was no significant differences among other groups (all P > 0.05). (3) The positive rate of HPV16 and the expression of Ki67 showed respectively positive being correlated with the expression of hTERT (P < 0.05, r=0.339; P < 0.05, r=0.398); HPV16, hTERT and Ki67 showed respectively negative correlation with the expression of p21waf1 (P < 0.05, r=-0.337; P < 0.05, r=-0.248; P < 0.05, r=-0.446); There was no significant relation between HPV16 and Ki67 (P > 0.05). CONCLUSION:The results suggest that in tissues of CIN and SCC changes in hTERT, p21waf1 and Ki67 expression may be associated with HPV16 infection and they interact with each other, which can influent the progression of CIN and carcinogenesis of SCC. These biomarkers may be analyzed comprehensively to reveal the detailed mechanism by which HPV16 participate in malignant transformation and to provide some informations on the diagnosis of patients with high risk for malignant progression. Tissue microarray is an efficient platform for high-throughput analysis of genes and their expression products in investigations.
Objective To investigate the relationship between expression of Bcl-2,Survivin and CD44v6 and HPV16/18 infection in uterine cervical carcinogenesis.Methods Formalin-fixed,paraffin-embedded samples from cervical carcer tissues were studied,the authors examined the expression of Bcl-2,Survivin and CD44v6 by using immunohistohemical technique.The number of apoptotic cells was assessed in situ by TUNEL method.The infection of HPV type 16,18 DNA were determined by PCR.Results There were significant differences for Bcl-2,Survivin,CD44v6 and AI between carcinomas,CIN and normal cervices,respectively.Expression of Bcl-2,Survivin,CD44v6 and AI were correlated with tumor grades,clinical stages and lymph node metastasis.The positive rate of HPV16/18 was significantly different among cervical carcinomas,CIN and normal tissues.The infection of HPV16/18 was associated with the expression of Bcl-2,Survivin and AI,respectively.Conclusion The abnormal expression of Bcl-2,Survivin and CD44v6 are associated with the infection of HPV16/18.They possibly become useful indexes for the primary screening and prognosis of cervical carcinomas.
目的:探讨米非司酮及米索前列醇与钳刮术结合终止10周～15周妊娠的临床效果.方法:选择我院2000年1月～2001年1月单胎妊娠10周～15周孕妇30例,口服米非司酮及米索前列醇后行钳刮术.结果:服药后宫颈口充分扩张且无阻力感,钳刮术操作时间短、出血少.结论:米非司酮及米索前列醇终止10周～15周妊娠,操作时间短、出血少、子宫收缩快,是简便、安全、有效的方法,值得推广应用.
Objective:To evaluate the results of combined chemotherapy with paclitaxel(PTX) plus cisplatin (DDP) in the treatment of advanced non-small cell lung cancer (NSCLC).Methods:Fron October 2003 to October 2005,31 patients with advanced non-small cell lung cancer received PTX 135 mg/m~2 by intravenous infusion on day 1,and DDP75 mg/m~2 by intravenous infusion on day 2～4.The treatment was repeated every 21 days,up to 2 cycles.Results:31 patients were evaluable for efficacy.12 got partial respons (38.2 %),with an overall rate of 38.2 %.The main toxicities were myelosuppression,nausea and vomiting,and other side effects were mild.Conclusion:A high response rate can be obtained in advanced NSCLC by PTX plus DDP.PTX is a promising antitumor agent with tolerable toxicity.
目的:分析应用动力髋螺钉(Dynamic Hip Screw,DHS)内固定治疗股骨粗隆间骨折的疗效.方法:对我院自2001～2004年间应用DHS治疗股骨粗隆间骨折42例患者的疗效进行回顾性分析,随访时间2～30个月.结果:所有病例术后均愈合良好,无髋内翻、下肢外旋及短缩畸形,患髋关节功能优良率达95.2%.结论:DHS内固定疗效肯定,是治疗股骨粗隆间骨折的理想方法.
OBJECTIVE:To confirm the relations between the expression of cyclin E, p16ink4, ki67 and HPV16/18 infection using cervical exfoliated cells, and evaluate the usefulness of cyclin E, p16ink4 and ki67 as biomarkers for screening of cervical carcinomas.METHODS:The expression of cyclin E, p16ink4 oncoproteins and ki67 proliferative activity was evaluated immunohistochemically in 78 cervical exfoliated epithelial specimens. Human papillomavirus type16 and 18 (HPV16/18) infection was assessed by polymerase chain reaction (PCR) using type specific primers.RESULTS:Cyclin E, p16ink4 and ki67 were all overexpressed in cervical preneoplasia and neoplasia cells, compared with little expressed in ASCUS (P less than 0.005). Overexpression of cyclin E was observed in CIN, (P less than 0.01), p16ink4 and ki67 overexpressed in invasive carcinoma(100 percent and 90.9 percent respectively). The degree of p16ink4 and ki67 expression correlated well with the degree of cervical neoplasia (P less than 0.005). HPV16 infection was assessed at all stages of cervical neoplasia samples, and a significant relationship with the degree of cervical epithelial lession was observed at the same time. The expression level of p16ink4 and ki67 seemed to be more closely associated with HPV16 infection than cyclin E did (rs=1.0 vs rs=0.4). HPV18 was found positive in only 1 case in CIN1 and in 4 cases in CIN2-3. Therefore no significance was found on statistical analysis (P less than 0.005).CONCLUSION:Cyclin E, p16ink4 and ki67 should be regarded as useful biomarkers of HPV-related cervical neoplasias, and be used for screening patients at high risk for developing cervical carcinomas. Moreover, cyclin E might be a significant cytologic marker for the primary screening of cervical carcinomas.
目的:总结手术和非手术治疗外伤性脾破裂的体会;方法:根据脾破裂损伤级别程度,结合其它合并伤的情况,对8例单纯脾破裂患者采用非手术治疗,对其余30例合并其它脏器损伤的脾破裂患者采用手术治疗;结果:8例非手术治疗患者和30例手术治疗患者中的28例治愈,2例死于严重性多种脏器损伤引发的大出血、脏器衰竭,治愈率为94.7%。结论:脾切除术治疗外伤性脾破裂见效快、疗效好、并发症少,对合并其它脏器损伤者应及早确诊,手术治疗。
近年来许多研究已证实HPV感染与宫颈癌的发生高度相关.随着宫颈癌细胞生物学和分子生物学研究的不断深入,宫颈癌细胞凋亡的研究已成为肿瘤生物学的重要组成部分.本文就学前HPV、宫颈癌与细胞凋亡三者的相关性研究作一综述.
目的:探讨儿童肘内翻的治疗方法。方法:在全麻下行肱骨髁上截骨植骨张力带固定术,术后肘关节屈曲位石膏固定4周后,再进行肘关节功能锻炼。结果:随访25例,其中1例发生再骨折,二次手术矫正。余均恢复到健侧携物角水平,肘关节活动正常,优良率95.5%。结论:传统手术仍有再移位的可能,我们对固定方法做了进一步的改进,避免了再移位畸形的发生,收到了很好的治疗效果。
近年来基因工程的飞速发展给医药科学研究及生产实践领域带来了革命性的变化。本文详细描述了基因工程技术的研究现状与应用前景,突出了该技术的优势之处。同时也分析了该技术在发展中存在的问题及不足,针对性地提出了建议及对策。
目的:探讨阑尾炎术后切口感染的治疗及预防。方法:将我科1995年8月-2005年10月施行阑尾切除术的1204例患者分成2004年以后组与2004年以前组进行比较。结果:经规范的治疗,2004年以后组切口感染的发生率较2004年以前组为低。结论:规范的治疗可明显降低术后切口感染的发生率。
目的:探讨剖宫产腹壁切口子宫内膜异位症的诊断和治疗。方法:回顾、随访、分析我院10年间诊治的13例剖宫产腹壁切口子宫内膜异位症患者的诊治过程。结果:经手术、服药后均可治愈、无复发。结论:该病应引起临床重视,尽量避免该病的发生。
Objective:To investigate how to distinguish and diagnose the chordoma.Methods:The clinical、X-ray plain film and pathological features of twenty-nine cases of chordoma were analyzed.Results:No distinctive symptomes occurred in these patients.Twenty-five tumors located in sacrum.The time of falling into disease range from half past four years to two months.In following up ten cases reoccurred.Conclusion:Chordoma has a high rates of reoccur.Chondroid metaplasia mayebe a sign of better prognosis.
目的:观察保妇康栓治疗妊娠合并念珠菌性阴道炎的临床效果。方法:阴道放置保妇康栓,每晚一粒,7天为一个疗程,共2个疗程。结果:保妇康栓治疗51例妊娠合并念珠菌性阴道炎的总有效率96.08%,其中痊愈64.71%,显效29.41%,有效1.96%,无效3.92%。结论:保妇康栓治疗妊娠合并念珠菌性阴道炎效果良好。
尿酸是人体内嘌呤碱的代谢产物,人血尿酸水平稍有差异,但含量是恒定的。近几年由于人们生活水平的提高,饮食结构的改变,嘌呤的摄入量也明显增加,导致高尿酸血症患者明显增多,引发了一系列疾病,如痛风等。同时血清尿酸含量增高也是诊断各种肾脏疾病、急性脑血管疾病、心力衰竭、糖尿病及其它慢性疾病的一项重要参考指标。
