Intestinal parasitic infections, particularly soil-transmitted helminths (STHs), remain a major public health concern in low- and middle-income countries. As control programs reduce infection intensity, accurate diagnosis becomes increasingly challenging. The McMaster technique has been proposed as an alternative diagnostic method for detecting human intestinal parasites; however, its diagnostic performance in human populations remains unclear. This study aimed to evaluate the diagnostic sensitivity of the McMaster technique for detecting intestinal parasites, particularly STHs. A systematic review and meta-analysis were conducted in accordance with the PRISMA guidelines and registered in PROSPERO (CRD420261298952). Electronic databases (PubMed, Scopus, Web of Science, Ovid, Nursing & Allied Health Premium, and Google Scholar) were searched up to January 2026. Studies assessing the McMaster technique in human populations and reporting sufficient data to calculate sensitivity were included. A random-effects model was used to estimate pooled sensitivity, with subgroup analyses by parasite species, geographic region, and population characteristics. Risk of bias was assessed using the the Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) tool. Among the 1514 records identified through database searching, 10 studies were included in the final analysis. The pooled sensitivity of the McMaster technique was 73% (95% confidence interval [CI]: 67–78%; I2 = 91.0%) for detecting any intestinal parasites and 72% (95% CI: 65–78%; I2 = 93.5%) for STHs. Species-specific sensitivities were 67% (95% CI: 61–72%; I2 = 80.7%) for Ascaris lumbricoides, 79% (95% CI: 57–91%; I2 = 96.9%) for Trichuris trichiura, and 71% (95% CI: 61–80%; I2 = 89.9%) for hookworms. The McMaster technique demonstrates moderate sensitivity for detecting intestinal parasites in humans. While it offers practical advantages for field applications, its reduced sensitivity in low-intensity infections limits its utility as a standalone diagnostic tool in control and elimination settings. Further research should focus on methodological standardization and integration with more sensitive diagnostic approaches.
Measles remains a public-health concern where immunity gaps and incomplete vaccination coverage persist. This study assessed household and geographic factors associated with laboratory-confirmed measles among suspected cases in three Zambian provinces. A prospective cross-sectional study was conducted during measles outbreaks in five selected districts across Luapula, Northern and Northwestern provinces. Suspected cases meeting the IDSR case definition were consecutively enrolled. Blood samples were collected, transported to the UTH Virology Laboratory, and tested using procured IgG test kits. Participant characteristics were summarised descriptively. Bayesian logistic regression estimated associations between laboratory-confirmed (IgG positive) measles and age, sex, vaccination status, household size and previous measles history, with province added in a second model. Results were reported as posterior odds ratios with 95% credible intervals. Among the 172 suspected cases, 45 (26.2%) were laboratory confirmed (IgG positive). Vaccination status was verified using caregiver-provided immunization cards; status was unknown for 110 (64.0%) participants (all of whom tested negative), reflecting missing vaccination documentation. Vaccinated participants had 93% lower adjusted odds of laboratory-confirmed measles than unvaccinated participants (adjusted posterior OR: 0.07, 95% CrI: 0.02–0.18). Adjusted posterior probabilities were consistently lower among vaccinated participants across all three provinces (34% to 49%) than among unvaccinated participants (86% to 92%). Age, sex and household size were not clearly associated with measles positivity. All four participants reporting a previous measles history tested positive, and the unadjusted association was significant using Fisher’s exact test (p = 0.004). Vaccination status was most strongly associated with laboratory-confirmed measles, with substantially lower adjusted odds among vaccinated participants. These findings reinforce the importance of measles vaccination in outbreak prevention and control.
Background: Dog-mediated rabies remains a fatal but preventable zoonosis. Its elimination requires sufficient canine vaccination coverage, informed communities, and spatially targeted surveillance. In Guinea, human knowledge, attitudes, and practices (KAP) data and canine serological data had previously been analyzed separately, limiting their translation into operational priorities. Objective: To build an integrated One Health assessment of rabies risk by combining KAP indicators, canine immunity, and spatial analysis. Methods: A secondary integrated analysis was conducted using a KAP survey of 2733 respondents and canine rabies serology from 419 vaccinated dogs tested with the Platelia™ Rabies II ELISA, with a protection threshold set at 0.5 IU/mL. Indicators were regionalized, compared, and incorporated into a vulnerability index. Associations were estimated by logistic regression with 95% confidence intervals, and spatial findings were displayed on a real map of Guinea. Results: Knowledge was insufficient or intermediate among most respondents, unfavorable attitudes reached 88.84%, and poor practices reached 73.80%. Declared canine vaccination coverage remained below the 70% programmatic threshold in all regions. Overall canine seroprotection was high (88.78%), but 11.22% of dogs remained insufficiently protected. Among dog owners, knowledge of rabies was associated with dog vaccination (OR = 2.77; 95% CI: 1.49–6.29). The integrated One Health assessment ranked Kindia as priority 1, Siguiri as priority 2, Boké and Labé as priority 3, and N’Zérékoré as priority 4. Conclusions: The integrated analysis shows that a strong immune response among vaccinated dogs is not sufficient when coverage, human practices, and the spatial distribution of risk remain heterogeneous. The recommended strategy is a regionally differentiated intervention combining post-exposure education, proximity-based dog vaccination, and targeted surveillance.
Background: Limited access to rapid molecular diagnostics remains a major barrier to timely tuberculosis (TB) detection and drug-resistance surveillance in resource-constrained settings. In the Republic of Congo, GeneXpert MTB/RIF platforms remain concentrated in a few laboratories, while peripheral facilities rely predominantly on smear microscopy, highlighting the need for a structured sample referral system. Methods: We conducted a three-year mixed-methods operational study comprising a six-month pilot phase (January–June 2023), initially enrolling 29 TB diagnostic and treatment centers in urban and rural settings followed by a 30-month national roll-out phase (July 2023–December 2025), evaluated retrospectively using routine programmatic surveillance data. Results: During the pilot phase, 962 sputum samples were transported. 294 (30.6%) were TB cases, including 12 (4.1%) RR-TB. Median total turnaround time was 53 h (19–168) in urban and 62 h (26–204) in rural, with 86% overall site participation (92% urban vs. 60% rural). During scale-up, samples increased from 2154 (2023) to 5160 (2024), achieving full national coverage (12/12 departments). Over three years, 2587 TB and 84 RR-TB cases were detected. The system’s contribution to national TB detection grew from 9.2% to 16.0%, and RR-TB detection from 10.4% to 18.7%. Conclusions: Implementation of the structured sample referral system was associated with improved equitable access to molecular TB diagnosis, turnaround times within national targets, and strengthened the RR-TB surveillance. Scalable transport networks integrated into national guidelines may enhance diagnostic equity and drug-resistance detection in centralized laboratory systems.
African swine fever (ASF) is a viral disease of domestic and wild pigs that has re-emerged as a major transboundary disease. Modelling using mechanistic, statistical, and machine learning (ML) approaches plays a key role in understanding ASF transmission and informing disease control, but the literature remains fragmented. To synthesise global ASF modelling efforts, we systematically reviewed studies applying these three approaches. We examined temporal and geographic trends, modelling objectives, explanatory variables, and model evaluation practices. A total of 151 papers published through 2024 met the inclusion criteria. Mechanistic (54.3%) and statistical (40.4%) approaches predominated, whereas ML (9.3%) was increasingly applied in recent years. Mechanistic models were primarily used to assess control strategies (48.8%) and transmission drivers (41.5%), statistical models to identify risk factors (63.9%) and spatiotemporal spread (32.8%), and ML for environmental suitability modelling (64.3%). Most were published from 2011 (99.3%) and focused on Europe (43.0%) and Asia (26.5%). Model evaluation remained inconsistent, with mechanistic papers frequently lacking model output uncertainty quantification (47.0%) and statistical papers often omitting model adequacy assessment (49.2%) and assumption checking (50.8%). Overall, ASF modelling approaches have developed complementary methodological roles, while geographic underrepresentation, limited representation of some transmission pathways, and inconsistent model evaluation remain important gaps.
Background: Blastocystis sp. is an anaerobic intestinal protozoan with extensive genetic diversity and controversial pathogenicity. The distribution and molecular subtypes of this entity in Guilan province, northern Iran, remain under-investigated. Objective: This study aimed to determine the prevalence and molecular subtypes of Blastocystis sp. in Rasht, Guilan Province, Iran. Methods: In this cross-sectional study (2023–2024), 300 stool samples were collected from patients referred to the medical laboratory at Razi Hospital. Microscopic examination and PCR amplification targeting the SSU rDNA gene were performed. Sequencing was performed on the positive isolates, and subtyping was achieved through BLAST alignment and subsequent phylogenetic analysis. Results: The overall prevalence of Blastocystis sp. was 12.3% (n = 37). No significant associations were found between infection and age, sex, or place of residence (p > 0.05). Molecular characterisation (23.08%). No statistically significant correlation was observed between subtypes and clinical symptoms, although ST1 and ST7 were more frequently detected in symptomatic individuals. Conclusions: Blastocystis sp. showed notable subtype diversity in the studied population, including the potentially zoonotic ST7. However, no significant association was found between subtypes and clinical manifestations. These findings support sustained molecular epidemiological surveillance and larger-scale studies to clarify subtype-specific pathogenesis and transmission dynamics.
Coral snakes of the American continent are classified into two genera, Micruroides and Micrurus, with as many as 91 species recognized in the latter. Although envenomings caused by Micrurus are rare, they can be life-threatening due to neurotoxic effects leading to flaccid paralysis and potential respiratory failure. Micrurus ancoralis is found in the Pacific lowlands and along the western slope of the cordillera occidental Cordillera in Colombia. Although this species is relatively common within its distribution range, its phylogenetic position and the characterization of its venom have not been investigated. The results of phylogenetic analysis placed M. ancoralis within the triadal/bicolor clade of species with the characteristic triad pattern and group bicolor. Proteomic characterization of the venom showed predominance of phospholipase A2 (PLA2) enzymes in its composition, with 51.7% of the total protein content, followed by three-finger toxins (3FTxs; 23.2%) and lower proportions of proteins belonging to several other minor families. Out of 28 chromatographic venom fractions obtained, the seven most abundant were evaluated for toxicity, with three of them (F7, identified as a 3FTx, and F18 and F20 (both identified as PLA2s)) showing lethal activity by the intraperitoneal route in mice. The PLA2 activity of F20 was confirmed and its edema-inducing, myotoxic, and lethal effects in mice were demonstrated. A therapeutic equine anti-coral antivenom (INS, Colombia) against coral snake envenomings immunorecognized the whole venom and its fractions in ELISA tests and was able to neutralize 2 × LD50 (medial lethal dose) of M. ancoralis venom by preincubation, with an estimated potency of at least 0.2 mg venom/mL antivenom. This result suggests that envenomings by M. ancoralis could be effectively treated by this antivenom.
Klebsiella pneumoniae (K. pneumoniae) is a leading cause of hospital- and community-acquired lower respiratory infections (LRI). The emergence of antimicrobial resistance (AMR), especially carbapenem-resistant strains (CRKP), poses a serious global health threat. Using Global Burden of Disease (GBD) 2021 data, we estimated AMR-related mortality for K. pneumoniae-associated LRI across regions from 1990 to 2021. Joinpoint regression analysis identified temporal trends. Global mortality declined overall, with a 70% reduction in under-5 deaths but a doubling among adults over 70 years. Deaths associated with and attributable to AMR decreased by 18.9% and 4.5%, respectively. The age-standardized mortality rate (ASMR) fell by nearly 50%. In contrast, the mortality burden associated with carbapenem resistance increased markedly, with AMR-associated deaths rising by 157% and disproportionately affecting high-burden regions such as Sub-Saharan Africa and South Asia. Despite overall progress, the increasing mortality burden associated with carbapenem resistance in K. pneumoniae-associated LRI poses a critical and persistent global health threat.
Background: Chikungunya virus (CHIKV) infection is an emerging disease of growing concern to international travelers due to the expanding geographic distribution of competent vectors, climate change, and frequent outbreaks in endemic and previously unaffected regions. Until recently, prevention relied exclusively on vector control and bite prevention; however, the recent approval of chikungunya vaccines has introduced a new preventive strategy. Methods: We conducted a narrative review of currently licensed chikungunya vaccines and candidates in clinical development, focusing on immunogenicity, safety, regulatory status, and implications for travel medicine practice. Results: Two vaccines have recently received regulatory approval: the live-attenuated vaccine IXCHIQ® and the virus-like particle vaccine Vimkunya®. Both demonstrated high immunogenicity based on neutralizing antibody thresholds accepted as surrogate markers of protection. Post-marketing safety signals associated with IXCHIQ®, particularly in older adults, have led to divergent regulatory decisions between authorities. The indication of vaccination in national recommendations varies somewhat depending on age, duration of travel, and individual risk factors. Several additional vaccine platforms remain under investigation, although some candidates have been discontinued. Conclusions: The recent approval of chikungunya vaccines marks a significant milestone in the field of travel medicine. However, uncertainties regarding long-term protection, safety in specific populations, and optimal targeting of travelers remain. Individualized risk–benefit assessment is essential, and further data are needed to refine vaccination strategies for travelers and populations at risk.
Antiretroviral therapy (ART) is essential for preventing HIV transmission and improving the health outcomes of people living with HIV (PLHIV). However, many barriers limit PLHIV from starting and adhering to ART, which explains why HIV responses in many settings, including Indonesia, have produced limited gains. This qualitative phenomenological study explored multilevel barriers to ART adherence in urban Yogyakarta (locally known as Jogja) and rural Belu, Indonesia, from the perspectives of PLHIV and healthcare professionals (HCPs). Data were collected through one-on-one in-depth interviews with 92 PLHIV and 20 HCPs. Participants were recruited using the snowball sampling technique. Data were analysed using framework analysis informed by the Access to Healthcare Framework. The findings showed that PLHIV in Belu and Jogja had different experiences in terms of the provision of and ability to access and adhere to ART or HIV treatment. In rural Belu, ART was less available and visible, harder to approach, often unaffordable, less aligned with patients’ needs, and strongly influenced by the widespread use of traditional medicine. PLHIV in Belu also reported a more limited ability to perceive the need for ART, reach services, pay costs, engage in care, and seek ART than those in urban Jogja. Personal, psychological, and social barriers were also reported to hinder PLHIV’s ART adherence in both settings. These findings highlight the need for HIV policies that promote the equitable distribution of ART services and targeted interventions to improve understanding and acceptance of HIV care among PLHIV and the wider community.
Integrated One Health studies are essential for assessing the risks of leishmaniasis transmission. In Honduras, the most prevalent clinical presentation of leishmaniasis is the non-ulcerated cutaneous form caused by Leishmania infantum; however, research on its transmission dynamics remains limited. This study aimed to characterize the eco-epidemiology of leishmaniasis and identify potential zoonotic risk factors in the municipality of Ojos de Agua, Department of Comayagua, central Honduras, using a One Health approach. We collected sand flies using CDC light traps placed in intra- and peridomestic settings of households with a history of leishmaniasis. Concurrently, we evaluated domestic dogs and collected blood samples for serological and molecular testing. We also conducted active surveillance among community residents to identify human infections. We collected a total of 644 phlebotomine sand flies, including 186 females. We identified eight sandfly species, with Lutzomyia (Lutzomyia) longipalpis as the predominant species, followed by Pintomyia (Pifanomyia) evansi. Among the 64 engorged females screened, the overall PCR positivity rate for Leishmania spp. was 14.1% (9/64). Blood meal analysis of PCR-positive sand flies identified humans and pigs as blood and meal sources. Additionally, 18.6% (8/43) of domestic dogs were seropositive for Leishmania spp., and 23.3% (10/43) tested positive by PCR. DNA sequencing identified the infecting species as Leishmania infantum. We detected no active human cases during the study period. These findings highlight the value of a One Health approach for improving transmission risk monitoring in newly emerging areas with a history of ulcerative cutaneous leishmaniasis in Honduras.
Although in Romania an increase in migrant population has been reported in the last years, there are no screening programs for infectious diseases implemented for migrants. We report the first two cases of leprosy (Hansen’s disease) since 1981 and the first imported ones in Romania, in two sisters originating from Indonesia, employed as massage therapists at a SPA center. Case one was diagnosed with multibacillary Hansen’s disease of mid-borderline type, and case two with paucibacillary Hansen’s disease borderline tuberculoid form based on epidemiological, clinical, histopathological and molecular diagnosis. Both patients have started multidrug therapy with dapsone, rifampicin and clofazimine provided by WHO. Both patients developed dapsone induced anemia under therapy, even though no G6PD deficiency was present, and dapsone was stopped. The clinical evolution of skin lesions was favorable under therapy, and the haemoglobin level started to increase after dapsone was stopped. This publication underlines the risk of importing rare diseases in Romania and in other European countries and the delay in diagnosis due to insufficient awareness among occupational medicine doctors. Improved strategies are needed at national level in order to increase awareness on rare imported infectious diseases and introduce screening programs in migrant population from high-risk areas.
The widespread circulation of the Anopheles vector is observed during the rainy season and clearly decreases during the dry season, when only a few mosquitoes are able to survive. This study aimed to identify the major species sustaining continued malaria transmission across seasons. This cross-sectional study was conducted in a malaria-endemic area. Mosquitoes were captured in Faladie using pyrethroid spray catches during rainy and dry seasons. The collected mosquitoes were identified using morphological keys, combined with molecular techniques such as PCR-RFLP (IGS regions) for Anopheles (An.) gambiae s.l. identification. Plasmodium spp. positivity in each mosquito was characterized by qPCR using parasite 18 S genes. A total of 1082 and 1705 mosquitoes were captured inside the same houses in the rainy and dry seasons, respectively. In the rainy season, 454 (41.9%) were Anopheles, 624 (57.7%) Culex and 4 (0.4%) Aedes. During the dry season, 65 (3.81%) mosquitoes were Anopheles, while 1,640 (96.19%) were Culex. Morphologically, the anopheline population consisted of 100% An. gambiae s.l. The Anopheles species diversity in the rainy season comprised the sister taxa, An. gambiae s.s. and An. Coluzzii, followed by an An. gambiae/An. coluzzii hybrid and An. arabiensis. However, An. coluzzii was found as the only vector across the rainy and dry seasons that sustained malaria transmission. Plasmodium falciparum infection rates were 9.7% and 1.7%, respectively, for the rainy and dry seasons. An. coluzzii species can survive both the dry and the rainy seasons and sustain malaria through cross-seasonal transmission. This highlights the importance of tackling An. coluzzii species and residual malaria transmission to eradicate the disease in endemic areas.
The World Health Organization targets hepatitis C virus (HCV) elimination as a public-health threat by 2030. Phetchabun province, in lower northern Thailand, has historically reported one of the country’s highest HCV seroprevalence rates. We describe a province-wide, decentralized test-to-treat micro-elimination program implemented within Thailand’s universal health-coverage system. Adults aged 35–64 years (2020–2024) and individuals born before 1992 (late 2023) across all 11 districts were screened using fingerstick anti-HCV rapid diagnostic tests at sub-district health-promoting hospitals. Reactive participants underwent confirmatory HCV-RNA testing at three regional nodes. Eligible patients received pangenotypic sofosbuvir/velpatasvir for 12 weeks, with ribavirin added for cirrhosis. Sustained virological response at 12 weeks post-treatment (SVR12) was the primary outcome. Of 400,567 targeted individuals, 389,547 (97.3%) were screened; 18,340 (4.71%) were anti-HCV reactive. Confirmatory HCV-RNA testing was completed in 14,845 (80.9%), of whom 10,996 (74.1%) had active infection. Of 8961 treatment-eligible patients, 8842 (98.7%) received DAA therapy. Among 6719 evaluable for SVR12, 6474 (96.4%) achieved cure. This simplified, domestically financed, decentralized test-to-treat model achieved WHO-target-level diagnosis, treatment, and cure rates at provincial scale, offering a scalable framework for HCV micro-elimination in low- and middle-income countries.
Background: Tetanus remains a life-threatening yet preventable disease, with a disproportionate burden in low- and middle-income countries. Data on non-neonatal tetanus in Somalia, particularly from referral centres managing severe cases, are limited. This study aimed to describe the clinical characteristics, management, and outcomes of patients with non-neonatal tetanus in Mogadishu, Somalia. Methods: This retrospective observational study was conducted at Madina Hospital between July 2024 and January 2026. All patients with clinically diagnosed non-neonatal tetanus presenting to the Emergency Department were included. Data on demographics, clinical features, management, complications, and outcomes were extracted from medical records. Continuous variables were summarized as mean ± standard deviation or median (interquartile range), while categorical variables were presented as frequencies and percentages. Associations were assessed using Fisher’s exact test, with p < 0.05 considered statistically significant. Results: A total of 50 patients were included (mean age 22.24 ± 15.49 years; 84.0% male). Most patients were unvaccinated (80.0%), and severe or very severe disease was present in 64.0% of cases. Generalized muscle spasm was the most common presentation (50.0%). Dysautonomia (42.0%) and respiratory failure (18.0%) were the most frequent complications. The overall in-hospital mortality rate was 26.0%, with respiratory failure accounting for 69.2% of deaths. Mortality was significantly associated with disease severity (p < 0.001), complications (p = 0.006), antibiotic category (p = 0.043), and vaccination status (p = 0.0265). Conclusions: Non-neonatal tetanus in this referral-based setting is associated with high mortality, predominantly affecting young, unvaccinated individuals presenting with advanced disease. Disease severity and complications, particularly respiratory failure, are the main drivers of outcomes. Strengthening adult immunization, improving early recognition and referral, and expanding access to critical care are essential to reduce tetanus-related mortality.
The Eastern Cape of South Africa carries the highest crude AIDS mortality rate nationally (135.86 per 100,000 in 2023), yet a province-specific, age-sex disaggregated analysis extending beyond 2012 does not exist. This study examined AIDS mortality trends in the Eastern Cape from 2000 to 2023. A quantitative ecological design was employed using secondary analysis of modelled estimates from the Thembisa Provincial HIV Model version 4.8. Annual AIDS deaths across nine age–sex subgroups and crude AIDS mortality rate were extracted from the Eastern Cape, 2000–2023, with provincial comparisons for 2023. Total AIDS deaths declined by 72.1% from 33,301 in 2005 to 9300 in 2023. Child deaths fell 96.1% from peak. Male youth deaths (15–24) plateaued after 2015. Adults aged 50 and older constituted 24.1% of deaths in 2023, with numbers increasing since 2019. Despite substantial gains, three unresolved gaps persist: a male youth mortality plateau, an ageing epidemic burden, and the highest provincial AIDS mortality rate nationally. Targeted differentiated interventions are urgently needed.
Cutaneous leishmaniasis (CL) is a vector-borne disease caused by Leishmania spp. Molecular methods such as conventional PCR (cPCR) and real-time PCR (qPCR) show high accuracy but remain restricted to reference centers. Loop-mediated isothermal amplification (LAMP) enables rapid DNA amplification under isothermal conditions. LAMP targeting 18S rDNA using WarmStart® Colorimetric LAMP 2× Master Mix and Bst 2.0 DNA Polymerase with SYBR® Green I (BstSg) was evaluated in swab samples (extracted by kit—SW-kit and direct boil—SW-DB) and specimens collected through biopsy from ulcerated lesions of patients attending a reference center in Rio de Janeiro, Brazil. The reference standard was defined as at least one positive parasitological or molecular (cPCR) test plus clinical confirmation. Analytical sensitivity reached 10 fg with WS and 10 pg with BstSg. The diagnostic accuracy of LAMP ranged from 51.8% to 78.6%, with sensitivity from 52.5% to 81% and specificity from 39.6% to 89.4%. LAMP, particularly when applied to swab samples with DNA extracted using the SW-kit protocol and amplified with BstSg, showed promising diagnostic accuracy and operational feasibility as a less invasive approach for CL diagnosis, eliminating the need for skin biopsy and reducing patient discomfort during sample collection.
(1) Background: Dog-mediated human rabies remains a serious public health problem in Cambodia, partially due to the high abundance of dogs. As most dogs are free-roaming and cannot easily be handled and restrained, the rabies vaccination coverage is very low. Oral rabies vaccination (ORV) could increase the vaccination coverage to levels required for interrupting the transmission cycle. (2) Methods: In this study, the bait acceptance and immunogenicity of a commercially available vaccine bait were tested in local free-roaming dogs in the northern part of Takeo province in Cambodia. (3) Results: In total, 75.4% (515/683) of dogs initially showed interest when offered an egg-flavored bait containing the SPBN GASGAS rabies virus vaccine. Subsequently, 89.9% of these dogs consumed the bait, and almost all of them (98.3%) were considered successfully vaccinated. Blood samples were collected from 50 dogs prior to vaccination. Eight of these fifty dogs tested sero-positive for rabies antibodies (ELISA) and were excluded from follow-up samples. Thirty and ninety days post-vaccination, 91.4% and 68.2% of the relocated vaccinated dogs tested sero-positive, respectively. (4) Conclusions: Bait acceptance and immunogenicity showed similar results as observed in studies with the same vaccine bait conducted in other countries. Hence, ORV seems to be a very promising tool for dog rabies control in Cambodia, which has a large owned but free-roaming dog population.
