We report draft genome sequences of Lactiplantibacillus plantarum LP48, Enterococcus durans ED01, and Bacillus subtilis BS01 isolated from artisanal goat cheese. Strains were identified by PCR, Sanger sequencing, and Illumina whole-genome sequencing. Assemblies revealed strain-specific features.
Here, we report the genome assembly and annotation of two clinical isolates of Naegleria fowleri from primary amebic meningoencephalitis cases in Costa Rica in 2020 using Illumina sequencing. Each assembly comprised 37 reference-guided scaffolds, with genome sizes of approximately 28 Mbp and a GC content of 36.87%.
The genome of Aspergillus ustus VKPM-F2030 strain was resequenced and assembled to achieve better quality and consistency of the assembly. It is the largest known genome of Aspergillus section Usti corresponding to 42 Mb and 25 contigs. This work can serve for further genomic investigations of Aspergillus fungi.
Here, we report the complete genome sequence of Sphingobacterium sp. PAMC25397, isolated from Ny-Ålesund, Spitsbergen, Norway. The genome is 5.2 Mb in length with a GC content of 37%. This genome harbors biodegradation-related genes involved in benzoate degradation.
We report the whole-genome sequence of multidrug resistant Escherichia coli RSJ-SAU.Ec.2025 isolated from the nasal swab of a cat in Dhaka, Bangladesh. The assembled genome comprises 5,318,660 bp with 50.5% guanine-cystosine (GC) content, together with putative antibiotic-resistant genes.
The draft genome sequence of Limimaricola soesokkakensis TX01, isolated from the light organ of Anomalops katoptron, which lives in the region of the Banda Islands, Indonesia. The assembled genome is 4,041,441 base pairs in length, distributed across 69 contigs, with a GC content of 67.21%, and encodes 3,823 predicted protein-coding genes.
We report the genome sequence of Breoghania corrubedonensis DSM 23382T isolated from oil-spill contaminated beach sand. The 5,331,589-bp genome with 63.62% G + C encodes 4,746 genes. This reference genome will facilitate experimental studies investigating B. corrubedonensis's role in oil-contaminated marine environments, particularly oil degradation or resistivity.
We report the complete genome sequences of three Pseudomonas isolates recovered from topsoil at the California Botanic Garden. Two isolates share ~99.4% average nucleotide identity, enabling investigation of intraspecies microvariation; the third represents a co-occurring distinct species, together capturing species- and strain-level genomic diversity within a natural soil community.
Metagenomic Coverage and Depth Calculator (Meta-CD) is a convenient, biologist-friendly tool for determining coverage and depth to enhance taxonomic detection, functional profiling, and metagenome-assembled genome (MAG) recovery in metagenomics. It supports experimental design and post-sequencing analysis, modeling how genome size, relative abundance, sequencing depth, and DNA quantity influence detection of target species.
Sediment microbial fuel cells (SMFCs) were constructed using different sediments, and microbial communities were analyzed by 16S rRNA gene amplicon sequencing. Methane concentrations were lower in roadside ditch sediment under SMFC conditions than under control conditions. Methanoperedenaceae was selectively enriched under SMFC conditions, suggesting a potential role in methane oxidation.
The Bacillus cereus group is ecologically diverse but genomically close and is known for its variety of capacities, including its antimicrobial potential. Within a project aiming to find new bioactivity, nine Bacillus strains were sequenced: eight Bacillus thuringiensis and one Bacillus mycoides. Here, we report their genome sequences and various genomic features.
In the current study, we present the genome sequence of Staphylococcus aureus isolated from a patient suffering from an ear infection. The bacterial genome was assembled into 26 contigs, comprising a total of 2,774,206 bp with a GC content of 32.5% and encoded approximately 2,782 genes, including multiple virulence-associated genes.
Barnettozyma species are members of the order Phaffomycetales, which include industrially important fungi. A species having ribosomal DNA similarity to Barnettozyma pratensis, but distinct from it, was isolated during collaborative identification work for a UK industrial source. Here, we report the draft genome sequence of Barnettozyma sp. NCYC 4493.
Rice is facing attacks from emerging diseases caused by fungi of the Curvularia genus. Twenty-one species have been isolated from rice. Of these, 8 species cause brown spot disease, and only 13 complete genome sequences are available. We produced 10 Curvularia genome sequences of strains isolated from diseased rice.
We report the near-complete genome of a divergent Megrivirus strain (avian/JF05/BA/BRA) detected in cloacal swabs collected from wild birds in Northeast Brazil. Genome assembly and annotation revealed a 9,680 bp RNA genome with moderate similarities up to 61.8% on the nucleotide level with members of the species Megrivirus aturhepa. The study highlights the importance of viral surveillance in wild birds.
DiMER (https://github.com/sandialabs/DiMER) is a modular Python framework using user-provided BLAST and HMM databases to assign annotations while avoiding placeholders (e.g., hypothetical protein). DiMER was applied to a curated list of gold standard mobile genetic elements and increased functional annotations by 24% (49 to 73%).
The Rhodotorula mucilaginosa strain AIRLRB09-2 is a laboratory isolate with proven capability of polyurethane and volatile organic compounds (e.g., acetone and styrene, degradation). Here, we report the draft genome sequence of this yeast isolated from airlift, the bioreactor for waste gas treatment containing acetone and styrene.
Here, we report draft genome sequences of two bacterial type strains isolated from cows, Pseudobutyrivibrio xylanivorans DSM 14809T and [Clostridium] fimetarium DSM 9179T. The P. xylanivorans DSM 14809T genome is 3.4 Mbp with 3,147 predicted genes, while the C. fimetarium DSM 9179T genome is 4.6 Mbp with 4,215 predicted genes.
Here, we report the complete genome sequence of Prevotella bivia GTC 18476, isolated from a clinical specimen in Nagano, Japan. The genome comprises two circular chromosomes and one plasmid measuring 1,298,955 bp, 1,212,313 bp, and 19,020 bp, respectively.
Bacillus velezensis strains MRL14-522 and MRL14-108 are plant-associated isolates with demonstrated biocontrol activity. This study presents the complete genome sequence of both strains. They consist of a circular chromosome (3,998,688 and 4,057,033 bp, respectively) and an intact prophage (NC_028969 and NC_029104, respectively).
