Objectives: This study aims to determine the frequency of KRAS mutations in patients with gastric adenocarcinoma (GAC) in Hatay province, and to determine the relationship of this mutation with some pathological and clinical parameters and to guide the diagnosis and treatment planning of patients. Methods: Formalin-fixed, paraffin-embedded, and histologically confirmed samples were used in the assessment of KRAS mutation. Sections were taken from the archive tissue samples of each case. Real-Time Polymerase Chain Reaction (RT-PCR) system was used to identify mutations of codons 12 and 13 (exon 2) of the RAS gene. Mutations of GLY12ALA (G12A), GLY12ASP (G12D), GLY12ARG (G12R), GLY12CYS (G12C), GLY12SER (G12S), GLY12VAL (G12V), GLY13ASP (G13D) were performed. Results: The mutation rate of KRAS was 2% and only one substitution, G12D, was detected. In this case, the tumor was located in the small curvature. Since the number of cases with mutations was low, statistical comparison could not be made between KRAS mutation and clinicopathological factors. A significant difference was found between tumor differentiation and WHO-2010 typing and primary tumor stage. Conclusions: We found the incidence of KRAS mutation to be 2%. We also estimate that the G12D mutation may be associated with GAC site and surgical margin. Although KRAS mutation in GAC alone is not a prognostic or predictive marker, subtype-specific analysis may provide data that may affect the diagnosis, management and treatment of the disease
Aim: To investigate the cognitive complaints and activities of daily living in patients with geriatric depression. Inter-group comparisons were performed by dividing geriatric depression patients into early- and late-onset subgroups. Materials and Methods: Ninety-four patients with geriatric depression and 39 individuals as a control group were included. Thirty-three of the geriatric depression patients had late-onset depression, and 61 had early-onset depression. All participants completed sociodemographic data, the Mini Mental Test, the Clock-Drawing Test, the Katz Activities of Daily Living Scale, and the Subjective Memory Complaints Questionnaire. Geriatric Depression Scale-15 was used to measure the severity of patients’ depression. Results: Late-onset depression patients exhibited significantly poorer performance on the Clock Drawing Test than the control group. Significantly greater subjective memory complaints were determined in patients with early- and late-onset depression than in the control group. A negative relationship was found between the severity of depression and activities of daily living in patients with geriatric depression. Conclusion: Geriatric depression may differ in terms of cognitive functions according to the age of onset.
Background: COVID-19; It is a pandemic disease that starts like a simple upper respiratory infection and causes pneumonia and severe respiratory failure. Due to cytokine storm and hyperinflamation, some hematological and biochemical findings appear in the disease. Complete blood count (CBC) is a very simple and inexpensive test that can be used in many infectious diseases. Methods: Eighty patients diagnosed with COVID-19 by RT-PCR were divided into two groups according to the presence of pneumonia in the Thorax CT (CT + 40 patients and CT- 40 patients). 40 healthy patients who had no previous disease were selected as the control group. The demographic and laboratory characteristics of COVID-19 patients with and without pneumonia in all three groups and control group patients were compared retrospectively. Results: While there was a significant difference between the COVID-19 group and the control group in terms of Neutrophil lymphocyte ratio (NLR) and Mean platelet volume (MPV), no differences were observed in COVID patients with and without pneumonia in terms of NLR and MPV. There was no significant difference between the COVID-19 group and the control group and the COVID pneumonia group in terms of Platelet lymphocyte ratio (PLR). Conclusion: NLR and MPV can be used as diagnostics for the diagnosis of COVID-19 disease, while PLR cannot be used. However, NLR and MPV values are not significant in evaluating COVID pneumonia and disease severity.
Amaç: Bu çalışma, cerrahi hastalarının hemşirelik bakımını algılayışını ve memnuniyet düzeylerini incelemek amacıyla yapıldı. Gereç, Yöntem: Tanımlayıcı ve kesitsel türdeki bu çalışmanın örneklemini, 17 Aralık 2018- 15 Mart 2019 tarihleri arasında bir üniversite hastanesinin cerrahi kliniklerinde yatan, araştırmaya katılmayı kabul eden, 18 yaş ve üzeri, iletişim kurabilen 300 hasta oluşturdu. Veriler, araştırmacılar tarafından oluşturulan “Hasta Bilgi Formu” ve “Newcastle Hemşirelik Memnuniyet Ölçeği”, “Hastaların Hemşirelik Bakımın Algılayış Ölçeği (HHBAÖ)” kullanılarak toplandı. Verilerin değerlendirilmesinde yüzdelik, ortalama, t testi, ANOVA, Karuskal Wallis testi, Mann-Whitney U testi ve Pearson’s Korelasyon analizi kullanıldı. Bu çalışma “Ege Üniversitesi Bilimsel Araştırma Projeleri (BAP) Koordinasyon Birimi”nce desteklendi. Proje Numarası: TLP-2019-20440. Bulgular: Cerrahi kliniklerde yatan hastaların; hemşirelik bakımından memnuniyet puan ortalaması 79.86±19.31 ve hemşirelik bakımını algılayışı puan ortalaması 68.03±9.87 olarak bulundu. Hastaların Newcastle hemşirelik bakımından memnuniyet ölçeği toplam puan ortalaması ile hemşirelik bakımını algılayışı ölçeği toplam puan ortalaması arasında istatistiksel olarak anlamlı bir ilişki olduğu saptandı (r= ,665; p= ,000). Cinsiyet, medeni durum, yaşadığı yer, sosyal güvence varlığı ve önceki hastane deneyimi ile bakımdan memnuniyet arasında anlamlı bir fark bulunmazken (p>0,05), eğitim düzeyi ve kronik hastalık durumu, hastaneye yatış şekli, odada bulunan hasta sayısı açısından anlamlı fark bulundu (p
Introduction: In the pathogenesis of the FMF disease, cytokines play important roles in the inflammation of the serous membranes. This study aims to investigate the relationship between serum levels of IL-1β, IL-18 and caspase-1 with CRP, ESR, fibrinogen and immune response attack/attack-free period. Material and Method: 60 patients diagnosed with FMF according to Tel-Hashomer criteria were divided into two groups as attack and attack-free period. Serum cytokine levels were examined by the ELISA method in 60 patients and 30 HCs, and the relationship between acute phase reactants and immune response was investigated. Results: A significant increase in serum concentrations of pro-inflammatory cytokines IL-1β (p = 0.001), IL-18 (p = 0.043) and caspase-1 (p = 0.043) was observed in FMF attack patients compared to control.. And also, IL-1β levels were positively correlated with neutrophils, ESR and CRP in FMF attack patients and there was a relationship between disease severity and IL-1β. In FMF attack-free patients, while a trend towards increased serum IL-1β (p=0.075) and IL-18 (p=0.516) levels were noted, it did not reach statistical significance. However, a significant difference was observed in the caspase-1 (p=0.049) levels of attack-free according to HCs. Conclusion: This study suggests that IL-1β, IL-18 and caspase-1 can serve as a useful marker not only in the attack period but also in the attack-free period, and provide a useful clue for the diagnosis and treatment of the disease.
