Abstract Embryo transfer (ET) is a foundational technique in mouse reproductive biology. Bilateral ET, in which embryos of different genotypes are placed in opposite uterine horns of a single recipient, offers strict control over maternal variables but requires that embryos remain in the horn to which they were transferred. Here, we tested this assumption directly by transferring BTBR T + Itpr3 tf /J (BTBR) and C57BL/6J (B6) blastocysts into opposite horns and genotyping mid-gestation fetuses by strain-specific PCR. Across four experiments varying embryo load, recipient strain, and donor-recipient synchrony, no genotype-discordant fetus was detected contralaterally. These findings validate bilateral co-transfer of genotypically distinct embryos as a methodologically safe design that halves the number of recipient females while providing rigorous within-dam experimental control.
Abstract The research examined the impact of prebiotic and organic acid additives, along with high stocking density, on the growth performance, biochemical parameters, histological characteristics, and immune function of local quail. A total of 276 one-day-old local quails were randomly assigned to five treatment groups, with three replicates per group and housed in boxes for 42 days. The dietary treatments were designed as follows: A negative control (12 birds/replicate), positive control (20 birds/replicate), group with 1% prebiotic (Jerusalem artichoke) additive at a high stocking density of 20 birds/replicate, group with 0.1% organic acid additive with high stocking density and a group with a combination of 0.5% prebiotic and 0.05% organic acid additives with high stocking density. The results showed that quails receiving prebiotic alone or a combination of prebiotic and organic acid supplements with high stocking density performed best compared to both control groups. The cholesterol and blood sugar levels were reduced in both the prebiotic alone and when combined with organic acid. The lactic acid bacteria count in the caecum digesta were significantly higher in the prebiotic alone and in combination with organic acid compared to both controls. The jejunum villus height was significantly increased with prebiotic alone and with prebiotic mixed with organic acid compared with both controls. All additives significantly enhanced the size of the Fabricius follicles. In summary, supplementing quail diets with prebiotics, alone or in combination with organic acids, under high stocking density conditions improved production performance, biochemical parameters, jejunum histology, and immune function in local quails.
Abstract Clarifying the relationships between cytokines, acute phase proteins (APPs), cathelicidins (CATHs), defensins, cytolytic proteins, and the proteins involved in lipid metabolism in the liver may improve existing immunotherapeutic strategies for various diseases or establish new ones. This study aimed to determine the effect of supplementation with dry turmeric-rosemary extracts on the relationships between lipid metabolism and immune system gene expression in the liver of young castrated Polish White Improved (PWI) goat bucks. The cytokine genes correlated both with those of other cytokines and with beta defensin 1 ( GBD1 ), as well as some CATH and APP genes. Most relationships were strong and positive. More associations were observed in the treated group than in the control group. Our results may mean that the tested supplementation may reduce the risk of hypertriglyceridemia and improve immunity against specific pathogens, its effect on the immune system is still unclear. Moreover, the used dose of the turmeric-rosemary mixture was too high and caused sensitization or the beginning of allergic inflammation. Further studies should include different doses of the supplement and confirm or reject our supposition.
Abstract The field of dermatological research has witnessed a rapid surge in interest surrounding exosomes – nanoscale extracellular vesicles with multifaceted biological functions. This bibliometric analysis, based on 171 publications extracted from the Web of Science Core Collection, explores the evolving landscape of exosome research in dermatology. Using VOSviewer software, we visualized term cooccurrence networks, identified key contributors, and mapped thematic clusters, revealing that exosome-related research has expanded significantly since 2018, with exponential growth in both publication output and citation frequency. China and the United States emerged as dominant contributors, while Europe and Oceania showed growing engagement. The Journal of Investigative Dermatology led in publication volume and citation impact, reflecting its central role in disseminating high-quality research in this domain. Keyword mapping highlighted critical research themes such as skin regeneration, wound healing, immune modulation, and therapeutic applications in chronic and inflammatory skin conditions. High-impact terms – including melanocyte, angiogenesis, mesenchymal stem cells, and microRNAs – reflect both foundational molecular mechanisms and translational relevance. Furthermore, emerging trends suggest increasing interest in plant-derived exosomes and their potential integration with technologies like laser therapy for enhanced transdermal delivery. Despite their promise in diagnostics, therapeutics, and cosmetic applications, challenges remain in standardisation, scalability, and regulatory validation. This study provides a comprehensive overview of the current state and future directions of exosome research in dermatology, offering valuable insights to guide future interdisciplinary innovations and clinical translation.
Abstract Digital advances are fundamentally reshaping the life sciences, with emerging technologies redefining research, diagnostics, agriculture, and biotechnology. This review provides an integrated overview of the key digital innovations shaping the field, including artificial intelligence and machine learning, Internet of Things-based sensing, blockchain, bioinformatics, big data analytics, digital twins, robotics, and social media-driven digital communication. These tools are examined across animal science, medical science, and biotechnology to enable precision agriculture, intelligent disease diagnosis, omics-based discovery, laboratory automation, and continuous real-time monitoring. Through the analysis of recent case studies, it is demonstrated how the combination of sensing, computation, and automation is driving more predictive, efficient, and data-centered life science systems. The review also addresses major challenges related to data governance, interoperability, ethics, and implementation. To identify relevant literature, a structured search was conducted across PubMed, Web of Science, Scopus, and Google Scholar, covering peer-reviewed publications from 2014 to 2026, with emphasis on articles published from 2020 onward. Search terms combined technology-specific concepts (e.g., artificial intelligence, Internet of Things, blockchain, bioinformatics, digital twin, and robotics) with life science application domains (e.g., animal science, medical science, biotechnology, precision livestock farming, and drug discovery). Studies were included if they reported primary findings, systematic reviews, or substantive conceptual analyses relevant to the application of digital technologies in life sciences; non-peer-reviewed sources were incorporated only when they represented major policy or institutional reports. Overall, this synthesis highlights both the transformative potential and the practical constraints of digital technologies in life sciences, offering a forward-looking perspective on the next generation of innovation in biologically informed digital systems.
