PurposeThe aim of this study is to evaluate in vitro the morphological and physicochemical effects of an experimental epigallocatechin-3-gallate (EGCG) gel and its combinationwith sodium fluoride (NaF) on the progression of dentin erosion.Materials and MethodsInitial lesions were induced in bovine dentin samples (n=10/group) and divided into: Control (gel without active ingredients), NaF (1.23% NaF), EGCG (10% EGCG), and EGCG+NaF (10% EGCG + 1.23% NaF). Half of the sample was protected and the other half treated and subjected to erosive/abrasive challenges (0.3% citric acid, pH=2.6 and simulated brushing). Volume loss (VL) and surface roughness (Ra) were evaluated by 3D laser confocal microscopy. Dentin composition was analyzed by Raman spectroscopy, considering mineral/matrix ratio (MMR), collagen organization, and crystallinity.ResultsThe EGCG+NaF group presented the lowest VL values (p<0.05). The Control group had the highest MMR values, while the EGCG+NaF group presented the lowest (p<0.05). For the Amide I/III ratio, the Control group had the lowest values and the EGCG+NaF group the highest (p<0.05). No statistically significant differences among the groups regarding the crystallographic order (p= 0.668).ConclusionThe combination of EGCG and NaF demonstrates the ability to mitigate volume loss under erosive-abrasive challenges, preserve structural integrity, and enhance collagen cross-linking within the demineralized organic matrix of dentin.
Purpose:Edentulism compromises oral function and quality of life. The All-on-4 concept offers a fixed alternative to conventional dentures under immediate loading. However, evidence on outcomes in anatomically challenging arches, particularly with dynamic navigation, remains limited. This study reports early clinical and radiographic outcomes, and the feasibility, of immediate-loading all-on-4 in a real-world setting using an anatomy-driven allocation strategy with freehand and navigation workflows. Materials and Methods:This retrospective cohort included consecutive patients treated between November 2023 and February 2025. Dynamic navigation was selectively applied in unfavorable arches; freehand surgery was used otherwise. Four implants per arch supported screw-retained provisional prostheses delivered within 24-36 hours. Primary outcomes were implant and arch-level survival and marginal bone level (MBL) change. Secondary outcomes included complications and provisional prosthesis survival. Results:Twenty-two patients (23 arches; 92 implants) were analyzed: 13 arches freehand and 10 navigation. Median follow-up was 5.11 months. Five implants failed (5.43%), all in the maxilla, affecting four arches (one navigation, three freehand). Implant survival was 94.23% (freehand) and 95.00% (navigation). MBL change was negligible, consistent with early remodeling and measurement variability. Biological complications occurred in 8 implants (8.70%), and two provisional fractures were repaired; 22 of 23 prostheses remained functional (95.65%). Conclusion:Immediate-loading All-on-4 with selective navigation achieved high short-term implant and prosthesis survival and minimal MBL variation. These findings support the feasibility of navigation in demanding arches in routine practice, warranting confirmation in prospective studies with longer follow-up.
PurposeThe aim of this study is to compare trueness and precision of dental models from three LCD 3D printers with different horizontal resolutions (2K, 4K, 8K) and to evaluate their clinical suitability for orthodontic use.Materials and MethodsThirty models were printed using Creality Halot One (2K), Phrozen Mighty 4K, and Phrozen Mighty 8K printers, with ten models each using manufacturer-recommended resins. Master digital models from intraoral scans were compared to re-scanned printed models through surface superimposition using Geomagic Control X software. Trueness and precision were evaluated by point deviation and Root-Mean-Square (RMS) analysis, with differences assessed by one-way ANOVA (p = 0.05).ResultsMean RMS values for 2K, 4K, and 8K printers were 0.266 ± 0.015 mm, 0.223 ± 0.017 mm, and 0.121 ± 0.012 mm, respectively (p < 0.001). The 8K printer showed highest trueness and precision, with deviations mainly in occlusal and gingival regions. The 0.25 mm error limit was within acceptable range for 4K and 8K models, while 2K models exceeded this value (p < 0.05).ConclusionPrinter resolution significantly affects dimensional accuracy. The 8K LCD printer demonstrated superior accuracy and reproducibility, proving reliable for orthodontic models and clear aligners.
Purpose:The aim is to evaluate and compare the effects of single versus repeated applications of potassium iodide (KI) on SDF-induced discoloration in primary canines. Materials and Methods:Thirty-six extracted human primary canines with dentinal caries were randomly allocated into three groups: Group I received 38% SDF alone; Group II received SDF followed by a single KI application; and Group III received SDF followed by immediately application after SDF and repeated on days 1, 3, and 5. Color change (∆E) was assessed on Days 1, 14, and 30 using a spectrophotometer based on the CIE Lab* system. Statistical analysis included One-Way ANOVA with Tukey post hoc tests for between-group comparisons, and Repeated Measures ANOVA with Bonferroni adjustment for within-group comparisons. Results:All groups showed significant differences in ΔE values at each time point, except between the single and repeated KI groups on Day 1. The Group I exhibited the highest ΔE values throughout the study, indicating the greatest discoloration, while Group III resulted in the lowest values. Over time, a progressive increase in discoloration was observed in Groups II, whereas partial color stability was maintained in Group III. Conclusion:Repeated application of KI after SDF treatment can result in a higher reduction in tooth discoloration compared to a single-application. This protocol may enhance the clinical acceptability of SDF, especially for visible anterior teeth in pediatric patients.
Purpose:To determine whether low serum vitamin D levels are inversely correlated with clinical parameters and positively correlated with gingival crevicular fluid (GCF) 25(OH)D3, 1,25(OH)2D3, LL-37 and tumor necrosis factor-α (TNF-α) levels in periodontitis patients with well-controlled type-2 diabetes (T2DM). Materials and Methods:Twenty-six well-controlled T2DM patients with periodontitis and Vitamin D insufficiency (Vit.D-IS group), and 15 well-controlled T2DM patients with periodontitis and Vitamin D sufficiency (Vit.D-S group) were included. Papilla bleeding index, bleeding on probing (BOP), supragingival plaque, clinical attachment level (CAL) and probing depth (PD) were evaluated. GCF total amounts of 25(OH)D, 1,25(OH)2D3, LL-37 and TNF-α were analyzed by ELISA. Serum 25(OH)D3 levels were determined using liquid chromatography tandem-mass spectrometry. Results:Whole mouth CAL and BOP scores were higher in the Vit.D-IS group than those of Vit.D-S group (P=0.038, P=0.048, respectively). Whole mouth PD and plaque values were comparable in both groups. Differences were not detected in GCF 25(OH)D3, 1,25(OH)2D3, LL-37 and TNF-α levels between Vit.D-IS and Vit.D-S groups. Serum 25(OH)D3 levels were adversely associated with CAL and BOP scores (P=0.018, P=0.049, respectively). Also, GCF 1,25(OH)2D3 total amounts were positively related with GCF LL-37 total amounts (P=0.0001). Conclusion:Serum 25(OH)D3 insufficiency was related to periodontal inflammation and destruction in periodontitis patients with well-controlled T2DM. However, GCF 25(OH)D3, 1,25(OH)2D3, TNF-α and LL-37 total amounts were not correlated with serum vitamin D levels. We have opinion that obtaining and maintenance of periodontal health should be included in the diabetic treatment protocol in Vitamin D deficient patients with periodontitis and well-controlled T2DM.
