Using SSR, a molecular marker, this study aimed to evaluate the genetic diversity and genetic relationship among 55 herbaceous peony (Paeonia lactiflora Pall.) cultivars belonging to the Lactiflora, Hybrid and Itoh Groups to lay a foundation for the molecular classification of peony cultivars, as well as for genetic background analysis and breeding of new cultivars. Our results show that 86 alleles, 76 of which were polymorphic, were obtained by screening 13 pairs of polymorphic primers from 100 initially tested primers, each of them having an average of 6.615 alleles. The range of variation of the effective alleles was 2.243-7.800, PIC content was 0.554-0.872 and Shannon?s genetic diversity index was 1.701-3.126. The 55 herbaceous peony cultivars were divided into nine groups based on UPGMA. This analysis indicates that the 55 cultivars have rich genetic diversity while a phylogenetic relationship of most cultivars was identified. This molecular-based classification is a useful and simple reference molecular method to differentiate peony cultivars in crossbreeding programs.
X-chromosome and its independent loci recently gained importance in genetic investigations concerned with disease control-case studies, population genetic analyses, and phylogenetic investigations. Infertility and lack of normal reproduction are important issues in health. Aazoospermia is a kind of infertility in men whose semen contains no sperm and can affect about 1-20% of the male population. The majority of cases of spermatogenic failure in humans are idiopathic, and the underlying causes are postulated to be genetic. We analyze the potential association between seven X-Chromosome SNPs and the neighboring sequences with azoospermia in four ethnic populations in Iran. We also carried out population genetic analyses to produce data on genetic variability, population structure, gene flow, and time of divergence in these ethnic groups, for the first time. As the X-chromosome genes/SNPs are in homozygous condition, for the association study we used multivariate statistical methods, which revealed the potential role of variable sites close to the studied SNPs in male infertility. Haplotype groups were identified for each of the seven SNPs. The results showed gene flow among ethnic groups due to migration and inter-breeding and Bayesian analysis of sequence data suggested divergence time for ethnic populations to be between 2-30 KY.
Cerebral Creatine Deficiency Syndromes (CCDS) are congenital metabolic disorders in the creatine metabolism pathway. In this study, we evaluated the clinical, phenotypic, radiological and genetic features of patients with CCDS. We tried to identify early diagnosis clues in patients. Especially, we reviewed the causes of delay in patients with late diagnosis. In line with these findings, the diagnosis is confirmed by enzyme tests and next generation sequencing based whole genome sequencing. In this study, 6 patients whose diagnosis was genetically confirmed were presented (5 GAMT mutations (someone is complete homozygous deletion in GAMT gene), 1 SLC6A8 mutation). 5 of these patients were from the same family, and 4 patients were patients with a late diagnosis. Two of the 4 patients who were diagnosed late were moderate and two had severe phenotype. The neurological findings consisted of patients with different clinical findings such as speech disorder, cognitive retardation, autism and epilepsy. Patients received appropriate treatment for the type of cerebral creatine deficiency. While response to treatment was good in early diagnosed cases, a partial clinical improvement was detected in cases diagnosed late. The patient, who was started treatment before neurological symptoms appeared, was neurodevelopmentally normal. It was observed that there was a strong relationship between age at diagnosis and phenotype and prognosis. We compared the clinical findings, phenotype and genotype characteristics of patients with CCDS. We reviewed the causes of delay in patients with late diagnosis. Thus, we wanted to raise awareness about early diagnosis and treatment of CCDS, one of the rare metabolic diseases.
The present research aimed to estimate the plant growth, fruit yield, seed and biochemical traits among the seven pepper parental lines and their six BC2F1 populations in order to generate information regarding the extent of genetic variability, heritability and genetic advance. The study was conducted using a randomized complete block design (RCBD) with three replications at the Vegetable Research Farm of Punjab Agricultural University, Ludhiana, India. The analysis of variance (ANOVA) showed significant mean squares due to parents and BC2F1 populations for almost all the studied traits, it indicating the presence of a sufficient amount of inherent variability among the parental line and developed populations which could be exploited via selection. The plant height, fruit weight, fruit width, number of lobes fruit-1, chlorophyll b, total chlorophyll, chlorophyll a and total fruit yield plant-1 were recorded high GCV and PCV among the parental lines and developed populations BC2F1, indicating higher extent of variability for these traits in the evaluated genotypes. The results revealed that traits with high GCV and PCV offer a better scope for improvement through simple selection. High heritability and high estimates of genetic advance were determined for the traits viz., plant height, fruit weight, ascorbic acid content and total fruit yield plant-1, in both parents and populations. Based on these results, we can conclude that these traits are under the additive gene effects and that above mentioned characters could be considered as a good selection indices in bell pepper.
This research was conducted to examine the fruit characteristics of selected walnut genotypes from the native population of Eastern Serbia. Over 28 trees that deserve attention were recorded through the selection procedure. By grafting, grouping and cultivation under the same conditions at the Fruit Research Institute in Cacak, seven promising genotypes with the most favourable fruit characteristics were identified. Important physical properties of the fruit, such as fruit weight, kernel weight, fruit dimensions and some kernel and shell properties were investigated. The fruits of the selected genotypes were large or very large. The fruit weight ranged from 13.4 to 17.9 g, whereas the kernel weight varied from 6.22 to 8.92 g. The kernel was light-coloured or yellow, of very good taste. The kernel percentage in the tested genotypes was high, over 50.0% (50.7-55.3%). Pearson's correlation coefficient revealed a significant correlation between fruit and kernel characteristics. PCA analysis showed an adequate grouping of genotypes based on fruit quality. The obtained results suggest that selected promising walnut genotypes showed very good fruit properties, and may contribute to the increase of walnut production in the future.
