Oral squamous cell carcinoma (OSCC) is a common malignant neoplasm of the head and neck. It exhibits aggressive invasive potential, and patients with advanced-stage disease tend to have poor overall survival rates. This study aimed to explore the expression levels and prognostic value of long non-coding RNA LINC01290 in OSCC and to clarify how this transcript modulates the malignant behaviors of OSCC cells. A total of 127 OSCC clinical specimens were enrolled, with matched tumor and adjacent non-tumor tissue specimens collected. RT-qPCR was adopted to quantify the expression of three molecules in clinical tissues and multiple OSCC cell lines. CCK-8 assays were performed to measure cell viability, Transwell assays were applied to evaluate cell migration and invasion capacities, and Western blot analysis was conducted to detect the expression levels of related proteins. Dual-luciferase reporter assays and Ago2-RIP assays were implemented to verify the targeted interplay among these genes. LINC01290 and interleukin 1 receptor antagonist (IL1RN) presented distinctly reduced expression levels, while microRNA-650 (miR-650) displayed increased levels in OSCC tissues and cell lines. Low LINC01290 levels represented an independent prognostic indicator for OSCC. In vitro assays confirmed that LINC01290 suppressed proliferation, migration, and invasion of OSCC cells. LINC01290 targeted and negatively regulated miR-650, and miR-650 further downregulated IL1RN. Rescue experiments demonstrated that miR-650 partially counteracted the tumor-inhibitory effects of LINC01290, and IL1RN overexpression reversed the oncogenic phenotypes induced by miR-650. LINC01290 upregulated IL1RN expression by sponging miR-650 and blocked malignant progression of OSCC. This transcript functioned as a prognostic biomarker and a potential therapeutic target for OSCC. N/A.
Mandibular asymmetry often persists after bimaxillary orthognathic surgery combined with mandibular contouring osteoplasty (MCO). This study aimed to evaluate the accuracy of computer-aided navigation (CAN) in transferring virtual surgical plans during combined bimaxillary orthognathic surgery and mandibular contouring osteoplasty (MCO), and to assess the postoperative mandibular symmetry achieved through this navigation-guided protocol. A prospective single-arm cohort study was conducted on 30 adult patients (13 males, 17 females; mean age 24.2 years) who underwent bimaxillary orthognathic surgery (Le Fort I osteotomy and bilateral sagittal split ramus osteotomy) followed by navigated MCO. Preoperative virtual surgical planning was performed using Mimics software. Intraoperatively, the maxillomandibular complex was repositioned under real-time CAN guidance (AccuNav-A system), and MCO was performed using a custom-designed percutaneous navigation probe. Mandibular symmetry was assessed pre- and postoperatively by measuring linear distances from bilateral reference points on the inferior mandibular border, as well as from maxillary and mandibular dental landmarks, to the Frankfort horizontal plane (FHP), mid-sagittal plane (MSP), and coronal plane (CP). The overall mean linear discrepancy between the virtual plan and the 12-month postoperative outcome was 1.92 mm, with angular deviations of 1.27° for the occlusal plane and 1.42° for the mandibular plane. For dental landmarks, maxillary points showed smaller deviations (mean 0.76 mm) than mandibular points (mean 0.98 mm), and deviations were smallest in the MSP (0.68 mm) and largest in the CP (1.03 mm). The 95
A rare disease is any condition that affects no more than one in every 2.000 individuals in a given population. These diseases have varied causes, which, in many cases, hinders early diagnosis and results in a poor quality of life. Due to their low incidence, the information available on these conditions is limited, highlighting the need to expand knowledge regarding rare diseases. The purpose of this study was to create a relational database containing information orofacial manifestations associated to rare diseases. Medical literature was systematically assessed to identify clinical signs and symptoms affecting the orofacial region. The findings were clustered to identify orofacial manifestations with common characteristics. In order to map the RDs and the related orofacial manifestations, the primary data source was the platform Orphanet, from which information about rare diseases, medical terminologies, and “Human Phenotype Ontology” (HPO) identifiers was retrieved. Data retrieval and wrangling were conducted in Python using the Spyder integrated development environment (IDE) within the Anaconda distribution. The relational data frame was modeled using MySQL Workbench. In total, 258 oral manifestations were classified in 11 groups. From all rare diseases, 863 presented at least one orofacial manifestation. Bone/skeletal manifestations were the conditions mostly associated with rare diseases, followed by oral mucosal manifestations, dental manifestations, craniofacial anomalies, and neoplasic manifestation. Within the limitations of this study, it was possible to map rare diseases presenting orofacial manifestations. Further development of the database is required to provide technical and scientific support to professionals.
Oral lichen planus (OLP) can cause persistent pain and impaired quality of life, but its long-term symptomatic course remains insufficiently characterized. We compared longitudinal pain trajectories and endpoint patient-reported outcomes according to subsequent corticosteroid treatment-response classification. Of 346 patients meeting electronic medical record-based eligibility criteria, 285 completed the January–February 2026 survey and formed the analytic cohort. Longitudinal Visual Analog Scale (VAS) scores were analyzed using a linear mixed-effects model with patient-specific random intercepts and slopes. Refractory OLP (ROLP) classification was derived retrospectively from treatment responses documented during follow-up. Clinically significant oral candidiasis was modeled as a visit-specific time-varying covariate. A day-90 landmark sensitivity analysis classified early treatment response using objective lesion response alone and analyzed subsequent VAS measurements. Endpoint oral health-related quality of life and psychological symptoms were assessed using OHIP-14 and HADS. The median longitudinal clinical observation period was 3.0 years (IQR, 2.3–3.8), and 65 patients (22.8
