Backround and Objective: Cydalima perspectalis is a major invasive species of Buxus colchica common in Georgia.It was decided to investigate the mortality of C. perspectalis using local entomopathogenic nematodes (EPN).Materials and Methods:To study the efficiency of local EPNs, was invaded the pest with the above mentioned nematodes and studied their effect on them.Use of identified effective species in the field experiments.These species of nematodes were first tested on C. perspectalis.The studies were carried out both in the laboratory as well in field conditions.Results: In the laboratory experiments, two different doses of a suspension of nematodes were used: 50 and 100 IJs/against of these five species, the highest insect mortality was due to the action of Heterorhabditis sp.(100%), Steinernema gurgistana (100%) and Steinernema thesami (97.4%).These three highly virulent species were used in the field trials at doses of 1000 and 2000 Ijs LG 1 water.In this case, Heterorhabditis sp. was less effective (56.5%) than S. thesami (85.5%) and S. gurgistana (78.1%).Conclusion: Studies showed that the local species of these nematodes used in the experiments (S. thesami and S. gurgistana) are characterized by the high pathogenicity of this pest.
Background and Objective: Vegetables are plant edible components that are eaten whole or in pieces, raw or cooked as part of a main dish or salad.The parasitic fauna of ten different vegetables distributed at Obollo-Afor Market, Enugu State, Nigeria, were investigated.The objective was to determine the prevalence of parasitic contamination of different commonly consumed vegetables sold at Obollo-Afor Market.Materials and Methods: The samples were centrifuged after being cleaned with normal saline and left to stand for 10-12 hrs to allow for sedimentation and the sediments were then inspected under a microscope.Using a modified Zieehl-Nelsen approach, the oocysts of the parasite Cryptosporidium parvum were found in vegetables.A questionnaire was used to gather information about the vegetables. Results:The overall prevalence of parasites found in the vegetables was 44 (36.7%).Ascaris lumbricoides ova (18.3%) was the most prevalent parasite whereas Trichuris ova (0.8%) was the least.False cubeb leaf (60%) was the most contaminated vegetable while African spinach (10%) was the least.As 17 (41.5%)vegetable sellers used clean water for irrigation, 1 (2.4%) used wastewater, 11 (26.8%)used animal (human) faeces as manure, 3 (7.3%)used organic manure whereas 4 (9.8%)used inorganic manure. Conclusion:There is a moderate prevalence of parasitic infestation, probably due to handling procedures from production to sales.
