The article presents basic principles of the developed concept aimed at improvement of system of epidemiological surveillance over hospital infections. The results of personal studies of ecologo-epidemiological peculiarities of hospital infections at multi-type hospitals, at outpatient-polyclinic institution and in case of interclinic spreading are given in the article. Characteristics of polygenic infections and the received data on microbial resistance to disinfectants used as scientific grounding of the proposed concept are presented. Epidemiological surveillance over hospital infections should be carried out on a case-by-case basis at medical and prophylaxis establishments and on the territory and should have adapted parameters in case of various types of medical care for population (in-patient and outpatient-polyclinic) depending on specialization (multi-type hospitals and specialized departments/clinics).