Инверсионная Вольтамперометрия Для Определения Цефалексина В Лекарственных Препаратах, Применяемых В Ветеринарии

Cephalexin-based drugs, produced in Russia, Belarus, Spain, China and India are registered in Russian Federation. Also a wide choice of products, the problem of drugs adulteration is not practically solved. This problem is compounded by the fact that for a number of antibiotics, including cephalexin are not developed control methods and standards. For the determination of cephalosporins in various objects immunological and microbiological express-tests, optical and chromatographic methods are using. Different variants of voltammetry (VA) may be used as alternative ones. VA method has the analysis and equipment low cost and combines a high information capacity with the simplicity of the signal measurement, because received voltammetric characteristic is used for identifying of substance as well as determination of its concentration. Namely, the position of the analytical signal (Ep) gives a qualitative characteristic, and its height quantitative. The aim of our work was to develop a voltammetric method for determination of cephalexin and analytical procedures for its control in pharmaceuticals used in veterinary medicine. During the study of model solutions the working conditions to obtain an analytical signal of the antibiotic at mercury-film electrode are determined: the potential electrolysis minus (0,35 ±0,05) V in supporting electrolyte 0.1 mol/dm 3 sodium citrate. Antibiotic concentration was determined by addition of certified mixtures and measuring of peak height in a potential range of minus (0,60±0,05)V. At the base on the fulfilled research for the first time developed voltammetric procedure for determination of cephalexin in aqueous solutions of drugs used in veterinary medicine in contents range from 0,1 to 2,0 g/dm 3 (Sr is not more than 15%) has been developed. The method is simple to perform, inexpensive and can be used for the detection of cephalexincontaining drugs counterfeit.