The optimized method of preparation of Glycyrrhizic Acid (GA) (90.5 ± 1.5%), the main triterpene glycoside of licorice roots ( Glycyrrhiza glabra L., Gl. uralensis Fisher) extract, and its trisodium and monoammonium salts has been developed based on the commercial licorice extract containing 20.0 ± 1.5% GA.