КОРРЕКЦИЯ ХРОНИЧЕСКОЙ ПЕЧЕНОЧНОЙ НЕДОСТАТОЧНОСТИ ПРИ ТРАНСПЛАНТАЦИИ КЛЕТОК ПЕЧЕНИ В ВИДЕ СУСПЕНЗИИ И КЛЕТОЧНО-ИНЖЕНЕРНЫХ КОНСТРУКЦИЙ (ЭКСПЕРИМЕНТАЛЬНОЕ ИССЛЕДОВАНИЕ)

On an experimental model of chronic fibrotic liver damage (male rats Wistar (n=60), damage of CCl 4 , the duration of the experiment 90 days) it was studied the effectiveness of cell therapy for the correction of chronic liver failure. These rats were divided into 3 experimental groups: in the I st group (control, n=10) isotonic saline (650 mkl.) was injected; in the II nd group (n=20) suspension of liver cells was applicated in a dose 8-10×10 6 cells; in the III rd group (n=30) suspension of liver cells and bone marrow cells (mesenchymal stromal cells) in ratio 5:1 were used as cell associates on microparticles injectable heterogeneous biopolymer hydrogel «SpheroGEL» (cell-engineering design) in common dose 8-10×10 6 cells . It was ascertained that in the 2 nd and in the 3 rd groups the accelerated normalization of disturbed liver functional indices (ALT, AST, ALP) took place – to 30 days, but in the control group only to 90 days. The reliable differences in rats of normalization of functional indices were absent between the II nd and the III rd groups. But in 90 days by using special histological dyeing it was found out that defibrotic processes in liver tissue were more expressed in the III rd group in comparison with the III nd group. Received results were consequence of prolonged vital activity of cells (liver cells and mesenchymal stromal bone marrow cells) into cell-engineering designs, which were transplanted in the III rd group. The obtained effect can be explained by that the developed cell-engineering designs provide adequate conditions for prolonged vital activity of the transplanted cells.