The present study investigated a genealogical structure of Kostromskaya cattle breed on blood groups in bulls-sires from the main farm bloodlines and related animal groups with different portion of Schwiss cattle blood used during the last years in breeding farms to improve milk productivity of Kostromskaya cattle. The spectrum of EAB-locus alleles of blood groups was analyzed and the genetic diversity, the degree of homozygosis and genetic consolidity of these genealogical groups were estimated. It was revealed, that Schwiss bulls-sires of American, Canadian and Austrian selection continue to influence, however, the studied farm lines and related groups have significant genetic distinguishes, confirmed by an index of genetic distance (D N = 0.647).