Высокочувствительный Неповреждающий Способ Выявления Состояния Митохондрий В Организме Путем Их Исследования Внутри Лимфоцитов Крови На Мазке Выявление Больших Различий При Лейкозах И Миопатиях У Больных Детей По Сравнению Со Здоровыми

The cytobiochemical assay is described in the paper which is elaborated by three of its authors. This method possesses high sensitivity in detection of the state of mitochondria in the organism by measurement of enzyme activity inside blood lymphocytes in smears. Lactate dehydrogenase, succinate dehydrogenase and their ratio LDH/SDH were investigated. The authors proposed to use this ratio as a quantitative indicator of so called Warburg effect, typical of cancer cells. Whereas LDH/SDH is 5.5-8.8 in healthy children, aerobic glycolysis and the indicator rise up to 16.5-55.2 in young patients with leucosis. In children suffering myopathy aerobic glycolysis falls down and the ratio is lower than in norm: 4.5 and less than one. The authors suggested the decrease of aerobic glycolysis under myopathy to be a biomarker of restorative biosynthesis and cell growth weakening inherent in this disease.