Результаты И Методика Сбора Стволовых Клеток Периферической Крови У Детей И Подростков

We report the data of 340 children with poor-prognosis solid tumors who had 592 PBSC harvests on cell separators after mobilization mainly by different treatment protocol chemotherapy regimens followed by G- or GM-CSF (94% of patients) or by G-/GM-CSF alone (6%). Timing of procedure was predicted by studying the blood count. When the WBC and platelet reached a median of 17,5 (0,9–109) and 182 (11–782) ×10 9 /L, respectively, the median number of 3,8 (0,1–22,7) ×10 6 CD34 + /kg with 1,6 (0,006–18,9) ×10 6 CD34 + /kg for 1 blood volume processed was obtained per procedure. In the group of 128 patients with low body weight [median 14,2 (8–20) kg] 176 LP were successfully performed. The extracorporal line was primed with donor RBC in the patients with the weight below 12 kg. We observed no difference in CD34 + content in harvests whether CSF was begun on d+1 or on d+3 after chemotherapy or in later in the phase of hematopoiesis recovery.