For the extraction-fluorimetric determination of nanogram amounts of codeine in human urine the use of the fluorescence of the toluene extract of the codeine ionic associate with eosin was proposed. The formed ionic associate was characterized by the intensive fluorescence with a maximum of 550 nm. It was shown that the fluorescence intensity of the codeine ion associate with eosin in toluene was 10 times higher than the intensity of its luminescence in carbon tetrachloride, and 3 times higher than its intensity in chloroform. The optimal conditions for the formation of the fluorescent ion associate of the positively charged codeine ion with a negatively charged form of eosin were determined. The maximum fluorescence intensity of the ion associate was observed during the toluene extraction from the aqueous solutions with pH of 6 - 8 and the eosin concentration in the range of 1·10 -5 -1·10 -3 mol·L -1 . The fluorescence intensity of the extract was maintained at the initial level for 24 hours. The extraction-fluorometric procedure for determining the nanogram amounts of codeine in human urine was developed. The limit of detection of codeine, calculated by 3s-criterion, was equal to 6 ng·ml -1 . The calibration curve linearity was maintained in the range of 30 – 320 ng·ml -1 . The procedure for the determination of codeine in real expert samples of urine was tested. The relative standard deviation did not exceed 0.08. The obtained results for the extraction-fluorimetric determination of codeine in human urine allowed recommending the developed procedure for its use in the solutions of certain tasks in the practice of the institutions of the corresponding profile. Keywords: Сodeine, determination, human urine, extraction, fluorescence (Russian) DOI: http://dx.doi.org/10.15826/analitika.2017.21.4.006 S.I. Metelitsa 1 , E.V. Kireeva 1 , V.V. Nemikhin 2 , S.V. Kachin 1 , V.N. Losev 1 , S.A. Sagalakov 1 1 Siberian Federal University, Svobodnyi pr., 79, Krasnoyarsk, 660041, Russian Federation 2 Krasnoyarsk Regional Bureau of Forensic Medical Examination, Mira pr., 35, Krasnoyarsk, 660049, Russian Federation
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