A Dual-Channel Fluorescent Probe for Monitoring Hydrogen Sulfide and Glutathione in Alcoholic Liver Disease and Inflammation Models

Alcoholic liver disease (ALD) is closely associated with oxidative stress, a process that not only exacerbates liver damage but also increases the risk of developing inflammation. Hydrogen sulfide (H2S) and glutathione (GSH), as key redox regulatory factors, play central roles in maintaining cellular redox homeostasis and regulating inflammation. However, due to their similar chemical properties, achieving simultaneous and highly selective monitoring of them still faces enormous challenges. To address this issue, we have developed a single-excitation, dual-emission fluorescent probe (YF-O) capable of selectively detecting H2S and GSH with distinct emission signals at 600 nm and 705 nm, respectively. YF-O exhibits high sensitivity, with detection limits of 0.035 mu M for H2S and 0.065 mu M for GSH, respectively, along with a rapid response time of 240 s and a large Stokes shift, enabling reliable discrimination between the two analytes. YF-O can sensitively respond to analyte fluctuations, making it suitable for real-time metabolic monitoring in cells and various in vivo models. Mouse models of ALD and acute inflammation were established for imaging application, and the results indicated that YF-O effectively monitored and reflected the dynamic changes in glutathione depletion and elevated hydrogen sulfide levels during the progression of ALD and inflammation. This highlighted the tremendous potential of YF-O as a dual-detection molecular probe, offering promise for monitoring redox-related pathological changes and evaluating therapeutic efficacy.