A Protein Interaction Map of Drosophila Melanogaster

L Giot,JS Bader,C Brouwer,A Chaudhuri,B Kuang, Y Li, YL Hao,CE Ooi, B Godwin, E Vitols, G Vijayadamodar,P Pochart, H Machineni, M Welsh,Y Kong, B Zerhusen, R Malcolm, Z Varrone,A Collis, M Minto, S Burgess, L McDaniel, E Stimpson, F Spriggs,J Williams, K Neurath, N Ioime, M Agee, E Voss, K Furtak, R Renzulli, N Aanensen, S Carrolla, E Bickelhaupt, Y Lazovatsky, A DaSilva,J Zhong,CA Stanyon,RL Finley,KP White, M Braverman, T Jarvie, S Gold, M Leach, J Knight,RA Shimkets,MP McKenna,J Chant,JM Rothberg

Science（2003）SCI 1区

CuraGen Corporation

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摘要

Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid–based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence was developed to refine this draft map to a higher confidence map of 4679 proteins and 4780 interactions. Statistical modeling of the network showed two levels of organization: a short-range organization, presumably corresponding to multiprotein complexes, and a more global organization, presumably corresponding to intercomplex connections. The network recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components. This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans.

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