The Lowry protein assay is a sensitive but highly nonspecific procedure. The standard Lowry protein assay has been modified so that protein can be assayed in the presence of interfering chemicals. The method is based on the observation that in the presence of 125 μg/ml of Na-deoxycholate, bovine serum albumin (5—50 μg) is reproducibly precipitated (90–104%) by 6% trichloroacetic acid. Interference by sucrose, glycerol, Tris—HCl, Tricine, and EDTA can be eliminated. Protein samples containing carrier ampholytes can also be assayed provided their NaCl concentration is adjusted to 1 m.
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