Deep eutectic solvents (DES) are emerging as promising eco-friendly solvents for various applications such as product extraction and quality preservation. However, their high viscosity may hinder their effectiveness. Therefore, incorporating water into DES can effectively mitigate this limitation. This study aims to assess how DES (choline chloride:glycerol (1:2)) hydration affects proteinous nitrogen diffusion, considering different extraction durations and mild temperatures (25–45 °C). The degree of DES hydration was identified as the most significant parameter influencing the extraction efficiency. The incorporation of high water content ( 50–70