Blue light (BL) plays an important role in stomatal opening, finely tuning plant responses to environmental conditions. While the BL signaling pathway is well understood in C3 and C4 plants, its role in crassulacean acid metabolism (CAM) plants remains uncertain. Traditionally, stomata in CAM plants were considered insensitive to BL stimulation, and as a result, research on such interaction was overlooked for a long time. Only recently, studies have found that the BL signaling cascade is active in CAM plants. Here, we investigated the effects of BL irradiance on stomatal responses in Agave, a highly productive CAM plant, by stimulating Agave leaves with BL and taking measurements of leaf gas exchange during the morning (closed stomata) and in the afternoon (open stomata). Our findings revealed that BL had no significant effect on stomatal opening during the morning period. However, BL increased stomatal conductance (gs) by 67.3