BUSCO: Assessing Genome Assembly and Annotation Completeness with Single-Copy Orthologs.

Felipe A. Simao,Robert M. Waterhouse,Panagiotis Ioannidis,Evgenia V. Kriventseva,Evgeny M. Zdobnov

Bioinformatics（2015）CCF BSCI 3区SCI 2区

university of geneva

被引用13194|浏览148

摘要

MOTIVATION Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50. RESULTS We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO. AVAILABILITY AND IMPLEMENTATION Software implemented in Python and datasets available for download from http://busco.ezlab.org. CONTACT evgeny.zdobnov@unige.ch SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.

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关键词

genome annotation,Quality Assessment,sequence alignment,Phylogenetic Analysis