Background and Objectives Bone marrow transplantation with umbilical cord blood (UCB) in adult recipients is limited mainly by a low CD34 + cell dose. To overcome this shortcoming by minimum manipulation, mineralized bone allograft (MBA) scaffold coated by UCB-USSC (unrestricted somatic stem cells) was used to expand CD34 + cells from UCB. Materials and Methods In this experimental study, UCB-USSCs were isolated and characterized by morphologic and immunophenotypical analysis. Then they were seeded on MBA (for 3D figuration) and culture plate (for 2D figuration) as a feeder layer. CD34 + cells were isolated from the UCB (by MACS method) and were expanded in 2D and 3D conditions for 3 weeks. At the end, cell count, flow cytometry, cologenic assay, and LTC-IC were done. Results After 3 weeks, ex vivo expansion of UCB-CD34 + was enhanced 250 ± 13.2 in 3D. The highest CFC expansion and LTC-IC were observed at day 14. Flow cytometry analysis showed the lowest percentage in 3D culture. Conclusions USSCs by 3D MBA structure produce the hematopoietic cytokine, extracellular matrix and binding molecules; they also create the intercellular interaction that can be used as a suitable feeder layer. It provides an ex vivo mimicry of bone marrow niche by enhancing surface/volume ratio. This model could be a suitable and alternative environment for HSCs expansion and hematopoiesis.
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