introduction: This study examines in vitro study of antioxidant activity of Cenchrus biflorus (CB) leaf, root extracts in various solvents (MeOH, Hydro-ethanol, Aqueous). materials and methods: The potential components present in Cenchrus biflorus root methanol extract (CBRME) were extracted by UV- Visible, FTIR, GC-MS along with anti-inflammatory properties. Antiradical function was estimated by DPPH, FRAP and Metal chelating assays. Anti-inflammatory properties of CBRME were assessed by carrageenan-induced paw edema model. results: Highest free radical scavenging activity were discovered in methanolic extract of leaf with minimum IC50 value (50.01 ± 0.002 µg/ml) succeeded by root methanolic extract with IC50 value (51.41 ± 0.008 µg/ml). Leaf methanol extract (227 ± 0.182) showed the maximum FRAP value followed by root methanol extract (203 ± 0.162) and leaf hydro-ethanol (155.2 ± 0.521). Leaf methanol extract of Cenchrus biflorus demonstrate higher metal chelating power (53.3 ± 0.022) as compared to the other extracts of Cenchrus biflorus. UV- visible analysis showed the presence of flavonoids, terpenoids and phenols in CBRME. FTIR showed alkyl amine, alkenes, carboxylic acids, alkenes, secondary amines, alcohols groups. GC-MS analysis of CBRME revealed 38 compounds. 10,13-Octadecadienoic acid, 9,11-Octadecadienoic acid, methyl ester, (E,E)-, 9-Octadecenoic acid (Z)-, Methyl ester were known as anti-inflammatory compounds. Significant reduction in edema of treated group were observed from 1 to 4 h in dose dependent manner (200, 300, 400 mg/kg). Blood serum parameters such as SGPT (51.8±1.48), AST (66±2.44) and ALP (149.8±2.38) were also restored to normal in treated group at high dose (400 mg/kg) as similar to standard group. discussion: In order to extract a high concentration of phytochemical compounds, antioxidants, and in vitro anti-inflammatory constituents from Cenchrus biflorus for use in pharmacognosy, methanol has been recommended as the best solvent. conclusion: Result highlighted CB as source of antioxidant and anti-inflammation properties for further studies.
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