Microorganisms inhabiting cold and oligotrophic aquatic environments experience persistent physiological stress, necessitating genomic characterization to understand their survival strategies. Psychrotolerant strains have evolved diverse metabolic adaptations, including secondary metabolite biosynthesis, which may contribute to environmental fitness and offer potential for low-temperature biotechnological applications. However, the genus Lacisediminihabitans remains poorly represented at the genomic level, limiting our understanding of its ecological roles and metabolic potential. To address this gap, we generated a high-quality complete genome of a psychrotolerant Lacisediminihabitans strain isolated from Antarctic freshwater. Lacisediminihabitans sp. FW035 grew at 2 − 25 °C with an optimum at 20 °C. The genome of strain FW035 is 3,842,169 bp in size with a G+C content of 66.4