Coupling of Enzymes to Proteins with Glutaraldehyde: Use of the Conjugates for the Detection of Antigens and Antibodies

Conjugation of peroxidase, glucose oxidase, tyrosinase and alkaline phosphatase to human immunoglobulin-G, human serum albumin, sheep antibody and rabbit antibody was carried out with glutaraldehyde. The conjugates retained a substantial part of their immunological and enzymatic activity. They were utilized for the intracellular detection of antigens and antibodies and they were employed for the characterization of the antibodies after immunoelectrophoresis.