Determining the Membrane Recruitment Mechanisms of Peripheral Membrane Proteins Through Native Mass Spectrometry Directly from Tunable Lipid Membranes

Transient association of peripheral membrane proteins (PMPs) with cellular membranes is vital to regulating a myriad of signaling pathways central to human health and disease. Increasing evidence suggests lipids play a pivotal role in regulating membrane recruitment, oligomerization, and activation of PMPs. Nevertheless, it remains experimentally challenging to detect these interactions and their effects directly from a native-like membrane environment. We have recently established a native mass spectrometry (nMS) method to study integral membrane proteins from tunable lipid bilayers and investigate membrane-associated oligomeric states and lipid interactors (Panda et.al.