Divergence in Isoprenoid Emissions among Subtropical Broadleaf Trees: Linking Photosynthesis, Leaf Economics, and Volatile Defense for Forest Management

Biogenic volatile organic compound (BVOC) emissions are pivotal in plant-environment interactions, yet their intrinsic linkages with photosynthesis and leaf economics across diverse tree species remain poorly integrated. This study investigated seven subtropical broadleaf species spanning distinct lineages to unravel how BVOC chemotypes (isoprene- vs. monoterpene-dominance) align with photosynthetic energy use and leaf structural traits. Using controlled measurements on excised branches to isolate physiological relationships, we combined TD-GC-MS, gas-exchange, and leaf trait analyses. The results revealed strong divergence in BVOC chemotypes that was broadly coherent with species phylogeny and ecological strategy. Isoprene-dominant (ISO) species exhibited higher photosynthetic rates (A), greater electron transport capacity (J max), and elevated J max/V cmax ratios, consistent with the hypothesis that isoprene synthesis functions as a photosynthetic overflow mechanism for energy dissipation. In contrast, monoterpene-dominant (MTS) species displayed higher specific leaf weight (SLW) and relied on stored and induced emissions, reflecting a conservative, defense-oriented strategy. BVOC profiles were strongly correlated with SLW and photosynthetic capacity, highlighting a coordinated 'structure-function-volatile' adaptive syndrome. These findings suggest that BVOC emissions may be embedded within leaf economic strategies and photosynthetic networks, providing a basis to help refine next-generation BVOC models by integrating leaf structural traits (e.g., SLW) and photosynthetic electron transport capacity (J max) into model parameterization.