Effect of Magnetic Field on the Ability of Vibrational Iterations to Enhance ROS Production by Neutrophils.

When studying high dilutions, we concluded that the main role in their activity is played by the vibrational effect accompanying the dilution of the initial substance during their preparation. This concept served as the basis for developing a novel "crossing" technology, in which the initial substance and a neutral carrier (water or lactose), placed in separate, closely spaced vials, are subjected to joint vibrational processing. Consequently, the neutral carrier acquires the capacity of exerting a modifying effect on the initial substance or its target, thereby altering its physicochemical and biological properties, with the resulting product termed a vibrational iteration. In this work, the influence of vibrational iterations obtained from a neutrophil suspension as the initial substance on reactive oxygen species (ROS) production by neutrophils was studied using a cellular chemiluminescence assay. The role of the magnetic characteristics of the fields under which the vibrational iterations were prepared was also investigated. It was shown that vibrational iterations prepared under geomagnetic field conditions exerted only a minor effect on ROS production by neutrophils. At the same time, vibrational iterations prepared under a combined magnetic field comparable to the geomagnetic background and supplemented with a low-amplitude variable component (50 nT with a frequency of 12.6 Hz) increased the ability of neutrophils to produce ROS by 44% ± 12%. These findings demonstrate the possibility of preparing vibrational iterations from various initial substances, including biological material. The modulating effect of combined magnetic fields on their activity was also demonstrated.