Embryo Transfer Following IVF Alters Susceptibility to Metabolic Phenotypes in Male Mouse Offspring Compared to Naturally Conceived Offspring.

Lay summary:The aim of this study is to highlight potential differences between embryos conceived naturally and those conceived via in vitro fertilisation (IVF). While the current scientific literature generally assumes that differences between these two modes of conception exist, many studies do not provide detailed phenotypic data, e.g. bodyweight, blood glucose and other observational metabolic health parameters. In addition, comparisons are often complicated by variations in choosing different strains of foster mothers to carry embryos. By addressing these limitations, our short summary and overview provide a well-defined comparison within a controlled experimental setup including a high-fat diet feeding period as metabolic challenge. We make the resulting data publicly available to offer a reference point for researchers using a specific mouse strain as fosters and to improve transparency regarding the specific differences in metabolic health observed between natural conception and IVF in this context.