Estimation of 8-Iso-pgf2α As a Marker of Oxidative Stress in T2DM Patients with and Without Dyslipidemia

Oxidative stress resulting from excessive production of reactive oxygen species (ROS) plays a pivotal role in the development of chronic diseases, including type 2 diabetes mellitus (T2DM) and its related metabolic disturbances. This study aimed to assess the biomarker 8-iso-prostaglandin-F2a (8-iso-PGF2a) as an indicator of oxidative stress T2DM patients and to investigate its association with dyslipidemia, with the objective determining its potential value in predicting early diabetesrelated complications. The study included 90 participants: 60 T2DM patients (30 with dyslipidemia and 30 without) and 30 age and sexmatched healthy controls. Fasting serum samples were analyzed for glucose and lipid profile using an autoanalyzer, while C-reactive protein (CRP) and 8-iso-PGF2a levels were measured using ELISA. Compared with healthy controls, patients with T2DM exhibited markedly elevated levels of oxidative stress and inflammatory markers, including significant increases in both 8-iso-PGF2a and CRP. Among individuals with T2DM, those with dyslipidemia showed substantially higher 8-iso-PGF2a concentrations and a slightly higher CRP levels compared with patients without dyslipidemia. Diagnostic assessment indicated that 8-iso-PGF2a showed moderate ability to distinguish T2DM patients with dyslipidemia from those without. These findings suggest that elevated levels of 8-iso-PGF2a, particularly in dyslipidemic T2DM patients, may serve as a promising early biomarker for assessing the risk diabetes-related complications.