Estimation of the Frequency of Inherited Germline Mutations by Whole Exome Sequencing in Ethyl Nitrosourea-Treated and Untreated Gpt Delta Mice

Germline mutations are heritable and may cause health disadvantages in the next generation. To investigate trans-generational mutations, we treated male gpt delta mice with N-ethyl-N-nitrosourea (ENU) (85 mg/kg intraperitoneally, weekly on two occasions). The mice were mated with untreated female mice and offspring were obtained. Whole exome sequencing analyses were performed to identify de novo mutations in the offspring.