Examining Alterations in Fgcm Concentrations Post‐defaecation Across Three Animal Feeding Classes (ruminants, Hindgut Fermenters and Carnivores)

Quantification of faecal glucocorticoid metabolites (fGCMs) is a popular non-invasive technique for monitoring wildlife's response to stressors, demanding an understanding of the stability of fGCM concentrations post-defaecation to ensure comparability of determined fGCM values across samples. To provide species-specific recommendations for the duration within which sampling can take place, we measured the rate at which the fGCM concentrations of nine different species changed throughout a 7-day period post-defaecation. In this study, we explored the temporal dynamics of fGCM concentrations in nine species across three feeding classes (ruminants, hindgut fermenters and carnivores): impala (Aepyceros melampus), giraffe (Giraffa camelopardalis), blue wildebeest (Connochaetes taurinus), plains zebra (Equus quagga), African elephant (Loxodonta africana), white rhino (Ceratotherium simum), cheetah (Acinonyx jubatus), spotted hyena (Crocuta crocuta) and leopard (Panthera pardus). Utilizing enzyme immunoassays already established for each of the focal species, we identified broader feeding class-specific patterns. All herbivores exhibited a significant decrease in fGCM concentrations over time, starting from 6 h (impala) to 48 h (giraffe, blue wildebeest, white rhino and African elephant) post-defaecation. For carnivores, concentrations remained fairly comparable for 12-24 h, after which fGCM concentrations either decreased (spotted hyena), increased (leopard) or remained stable (cheetah), with notable variation in triplicate concentrations (cheetah and leopard). These findings offer insights into scheduling faecal sampling for endocrine monitoring, particularly from free-roaming wildlife, to ensure comparability of determined hormone metabolite concentrations. Furthermore, the species-specific variation in fGCM concentration post-defaecation demonstrated in this study underlines the necessity to investigate every new species to ensure accurate and comparable results. Future studies ought to investigate how the mass of collected material, sex and drying methodologies affect the measurement of fGCMs post-defaecation.