Low-Cost Methods for Molecular Characterization of Mutant Plants(2015)
International Atomic Energy Agency
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摘要
Standard agarose gel electrophoresis is a quick method for the evaluation of the quality and quantity of DNA. This chapter provides examples of genomic DNA produced using the low-cost extraction protocol, PCR amplification using the extracted genomic DNA, and enzymatic mismatch cleavage of PCR products with crude celery juice extract and weed juice extract to detect mutations.