Aims] Skyrunning (SR) is an extreme sport of mountain running above 2,000 meters, and the number of participants in SR is rapidly increasing in Europe.In Japan, the sport has been growing in popularity since the establishment of the Japan Skyrunning Association (JSA) in 2013.However, the physical loads during SR remain unknown.The purpose of this study was to examine the effects of SR on runners.[Methods] 7 healthy males and females performed skyrunning (from 1,500 m to 2700 m in altitude) followed by a pre-test (20 m shuttle-run, weight bearing index, vertical jump, muscle hardness and blood, saliva and urine sampling).The data was analyzed as the trained group (TR: n=4, 2 males & 2 females) and the untrained group (n=3, 2 males & 1 female).[Results] There was no significant difference in mean HR during SR, however %V ・ O 2max indicated a high value in TR (p<0.05).And there were no significant differences in vertical jump, muscle hardness and oxidative stress markers, however WBI was high in TR compared with in the untrained group (p<0.05).[Conclusion] Compared with the untrained group, TRs performed continuously high intensity exercise with an equal HR level in a hypoxic environment.