Figure 1 from E3 Ubiquitin Ligase Mind Bomb 1 is a Novel Negative Regulator of Tumor-Suppressive BMP Signaling

MIB1 is a strongly selective cancer dependency linked to negative regulation of TGF-β family signaling. A, Gene dependency scores (gene effect score) for a subset of cancer cell lines in the CRISPR (DepMap Public 22Q2, Chronos) dataset exhibiting selective MIB1 dependency. For context, dependency scores for an essential gene (RPL14) and a nonessential gene (UCP1) are also shown. B, MIB1 dependency scores from DepMap (Public 22Q2, Chronos) for 1,086 cancer cell lines grouped by lineage. Each circle represents an individual cell line; box-and-whisker plots summarize the distribution across lineages. C, Top 10 MIB1 correlates based on DepMap (Public 24Q2 Chronos and Expression). Expression correlates (#): genes whose transcript levels correlate with MIB1 dependency; codependency correlates (^): genes whose presence or absence correlates with MIB1 dependency. MIB1 anticorrelates are shown on the left (green shaded boxes, solid outlines); correlates are on the right (red shaded boxes, dashed outlines). D, Simplified overview of the TGF-β family signal pathway with MIB1 correlates highlighted at their points of impact. Data are presented as the mean ± SD; n = 3 (two-way ANOVA with Tukey correction). [D, Created in BioRender. Cottonham, C. (2026) https://BioRender.com/ydae6kh.]