Figure 8 from E3 Ubiquitin Ligase Mind Bomb 1 is a Novel Negative Regulator of Tumor-Suppressive BMP Signaling

MIB1 and BMPR2 associate in cancer and endothelial cells. A, Co-IP of endogenous BMPR2 and MIB1 from NCIH838 cells treated with CLQ (25 μmol/L) or MG132 (10 μmol/L) for 6 hours prior to lysis to stabilize BMPR2 protein levels. IB confirmed co-precipitation of MIB1. Dox, used to induce KO of MIB1. B, Co-IP of endogenous BMPR2 and MIB1 from HUVECs transfected with control or BMPR2 siRNA for 72 hours. IP and IB were performed as described in A. C, Schematic (top) illustrates the experimental workflow for pull-down of endogenous MIB1 from HUVEC lysates using recombinant His-tagged BMPR2. Association of MIB1 with BMPR2 was verified by IB.