Figure 2 from Extracellular Vesicle Secretion by Leukemia Cells in Vivo Promotes CLL Progression by Hampering Antitumor T-cell Responses

LME-sEVs present a specific proteome and miRNA fingerprint. A, Hierarchical clustering of sEVs differentially expressed proteins (DEP with q < 0.05) identified by mass spectrometry between HCME-sEVs (n = 3, isolated from independent pools of 5 C57BL/6 spleens) and LME-sEVs (n = 14). B, Volcano plot showing DEP between LME- and HCME-sEVs with FDR <0.05 and log2FC >1. C, PCA based on DEP. D and E, Ontology analysis of DEP between LME-sEVs and HCME-sEVs. F, Expression of ICP ligands on HCME- or LME-sEVs quantified by bead-based FC. G, Representative pictures of ICP ligand expression on single MB488+CD20+ LME-sEVs visualized by imaging FC. H and I, HSNE clustering of MB488+ LME-sEVs based on CD20, PD-L1, GAL9, and MHC-II expression analyzed by FC and related combinations of markers on CD20+ LME-sEVs. J, miRNA levels measured using RT-qPCR from HCME- (isolated from a pool of 5 C57BL/6 mice spleens) and LME-sEVs (n = 8). Data are mean.