First Report of Alternanthera Yellow Vein Virus from Eclipta Prostrata in Pakistan.

HomePlant DiseaseVol. 101, No. 1First Report of Alternanthera yellow vein virus From Eclipta prostrata in Pakistan Previous DISEASE NOTES OPENOpen Access licenseFirst Report of Alternanthera yellow vein virus From Eclipta prostrata in PakistanS. S. Zaidi, S. Shakir, M. Farooq, I. Amin, and S. MansoorS. S. ZaidiSearch for more papers by this author, S. ShakirSearch for more papers by this author, M. FarooqSearch for more papers by this author, I. AminSearch for more papers by this author, and S. MansoorSearch for more papers by this authorAffiliationsAuthors and Affiliations S. S. Zaidi , Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan S. Shakir , Virology Laboratory, Center for Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad, Pakistan, and Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan M. Farooq I. Amin S. Mansoor , Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan. Published Online:10 Nov 2016https://doi.org/10.1094/PDIS-08-16-1164-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Alternanthera yellow vein virus (AlYVV) is a Begomovirus species that is transmitted by the silverleaf whitefly Bemisia tabaci. In July 2015, during a field survey of the Tando Mohammad Khan area in Sindh province and the Faisalabad area in Punjab province, Pakistan, symptoms of vein yellowing and swelling were observed on leaves of Eclipta prostrata (family Asteraceae). E. prostrata, also known as false daisy or bhangra,is a common weed in Pakistan and was recently reported as a host for AlYVV in China (He et al. 2008). To detect the possible presence of AlYVV, two symptomatic plants and one asymptomatic plant were collected and total DNA was extracted. Southern blot hybridization was performed using a DIG labeled probe to the AlYVVC1 gene. A strong hybridization signal was observed in symptomatic plants, while there was no signal in asymptomatic plants collected from the same fields. To further confirm the association of AlYVV, rolling circle amplification (RCA) was performed to enrich the circular DNA molecules of begomovirus genome, using phi29 DNA polymerase (Thermo Fisher Scientific, Waltham, MA). The RCA product was purified and sequenced by the Illumina MiSeq sequencing system. The Illumina NeoPrep automation system (Illumina, San Diego, CA) was used with the Illumina TruSeq Nano DNA Library Prep Kit (NP-101-1001). After sequencing, adapters were trimmed by the MiSeq Reporter Software and sequencing data were analyzed by CLC Genomics Workbench 7.5 (https://www.qiagenbioinformatics.com). De novo and reference-based assemblies were prepared following the standard quality parameters (quality score: 0.001 and Phred score: 30). The NCBI-nBLAST results of 2,917 assembled reads confirmed the presence of AlYVV, available in the database with the accession number KX710155. This AlYVV sequence showed 96% nucleotide identity with AlYVV (FN432361) reported on Sonchus arvensis from Faisalabad, Pakistan; another weed species belonging to the family Asteraceae. The species demarcation of AlYVV was also confirmed using the sequence demarcation tool (SDT) program (http://web.cbio.uct.ac.za/∼brejnev/), following the revised taxonomic criteria of begomoviruses (Brown et al. 2015). Neither in the Illumina data nor by PCR using universal primers for amplification of DNA-B (Zaidi et al. 2016), alphasatellites (Bull et al. 2003), or betasatellites (Briddon et al. 2002), was evidence found for the presence of DNA-B, alphasatellites, or betasatellites. E. prostrata has been reported as a host for multiple begomoviruses like AlYVV (He et al. 2008), and could therefore serve as an alternative virus reservoir, with an important role in the epidemiology of the disease. To our knowledge, this is the first report of AlYVV on E. prostrata in Pakistan.References:Briddon, R. W., et al. 2002. Mol. Biotechnol. 20:315. https://doi.org/10.1385/MB:20:3:315 Crossref, ISI, Google ScholarBrown, J. K., et al. 2015. Arch. Virol. 160:1593. https://doi.org/10.1007/s00705-015-2398-y Crossref, ISI, Google ScholarBull, S. E., et al. 2003. Mol. Biotechnol. 23:83. https://doi.org/10.1385/MB:23:1:83 Crossref, ISI, Google ScholarHe, Z. F., et al. 2008. J. Phytopathol. 156:496. https://doi.org/10.1111/j.1439-0434.2007.01381.x Crossref, ISI, Google ScholarZaidi, S. S., et al. 2016. PLoS One 11:e0155520. https://doi.org/10.1371/journal.pone.0155520 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 1 January 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 22 Dec 2016Published: 10 Nov 2016First Look: 17 Oct 2016Accepted: 23 Sep 2016 Pages: 266-266 Information© 2017 The American Phytopathological SocietyCited byNigrospora sphaerica causing leaf spot in a new host, Eclipta prostrata (False Daisy), in China20 January 2022 | Journal of Phytopathology, Vol. 170, No. 4Alternanthera yellow vein virusCABI Compendium, Vol. CABI CompendiumEvolutionary Factors in the Geminivirus Emergence13 July 2019Eclipta prostrata (False daisy)6 June 2020Bemisia tabaci-mediated facilitation in diversity of begomoviruses: Evidence from recent molecular studiesMicrobial Pathogenesis, Vol. 123Engineering Molecular Immunity Against Plant Viruses