High-quality Genome Assembly and Annotation of Inactivated Animal Vaccine Bacteria Strains in South Korea

Abstract Objectives Production of high-quality biological products depends on the careful selection and consistent management of seed strains. In this study, we generated complete genome sequences of bacterial strains employed in inactivated animal vaccines in South Korea and comprehensively examined their gene annotations. The genome data thus obtained provide a solid basis for confirming, at the strain level, whether manufacturers are using the same seed strains over time. In addition, these data offer a useful reference resource to support stable large-scale production and quality control of biological products. Data description The complete genomes of eleven bacterial strains employed for inactivated vaccine production were generated and annotated. A hybrid assembly workflow combining Illumina short reads (NovaSeq 6000) with Oxford Nanopore long reads (MinION) produced high-quality, gap-free genomes for all strains, with genome sizes ranging from 2.28 Mb to 5.35 Mb. The strains comprised Pasteurella multocida (D, 3A, A), Actinobacillus pleuropneumoniae (2 and 5), Glaesserella parasuis 4, Mannheimia haemolytica KO, Avibacterium paragallinarum C, and three Escherichia coli strains (F41, YC21-F17, K99S). All assemblies exhibited high completeness (>99%) and minimal contamination (<1%), ensuring reliable downstream genomic characterization.